Boletus aereus mother strain long-time preservation method

A technology of black boletus and female species, which is applied in the field of long-term preservation of black boletus female species, can solve the problems of unsuitable preservation, fast growth of mycelium, and large mycelial damage, so as to maintain mycelial vitality and bacterial growth. Effects of various cultivation traits, damage reduction, resistance to degradation and variation

Inactive Publication Date: 2015-06-03
JINGHONG HONGZHEN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Preserved strains are often used to grow on slopes or flat plates. Some other edible fungus mycelium grows fast, and it only takes about 10 days to grow on slopes or flat plates, and the mycelium is strong, while this strain takes 25-30 days. If the size is too large, the mycelia will be greatly damaged when the cell sap crystallizes, so it is not suitable for preservation
Therefore, the medium and low temperature edible fungus liquid nitrogen preservation method is not suitable for this strain, and it is urgent to find a method suitable for the long-term preservation of the black boletus strain, improve the stability of the strain, and provide a basis for the development and realization of the black boletus. Its industrial cultivation provides strain protection

Method used

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  • Boletus aereus mother strain long-time preservation method
  • Boletus aereus mother strain long-time preservation method
  • Boletus aereus mother strain long-time preservation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Inoculate the mycelium block on the stock culture medium, culture at a constant temperature of 30°C, and obtain the stock boletus for preservation in 15 days, wherein the stock culture medium contains the following components:

[0036]

[0037]

[0038] The color of the obtained black boletus parent species is milky yellow to golden, with sterile skin, sterile strands, no water spit, and good ductility;

[0039] Make the mycelium block of the black boletus parent species with a length and width of 0.9cm×0.5cm, and put it into a sterilized low-temperature freezing tube according to aseptic operation. The specification of the low-temperature freezing tube is 2ml, internal rotation, The cryopreservation tube contains 0.5ml of sterile 20% trehalose; after tightening the tube stopper, seal it with medical tape for two laps, shake it slightly, so that the mycelium block is completely immersed in the protective agent, and put one of the cryopreservation tubes into the bac...

Embodiment 2

[0043] Inoculate the mycelium block on the stock culture medium, culture at a constant temperature of 29°C, and obtain the stock boletus for preservation in 18 days, wherein the stock culture medium contains the following components:

[0044]

[0045]

[0046] The color of the obtained black boletus parent species is milky yellow to golden, with sterile skin, sterile strands, no water spit, and good ductility;

[0047] Make the mycelium block of black boletus parent species with a length and width of 1cm×0.3cm, and put it into a sterilized low-temperature freezing tube according to aseptic operation. The specification of the low-temperature freezing tube is 2ml, internal rotation, low temperature The cryopreservation tube contains 1ml of sterile 30% trehalose. Tighten the tube stopper and seal it with medical tape for two laps. Shake slightly to make the mycelium block completely immersed in the protective agent. One of the cryopreservation tubes is put into the bacterial...

Embodiment 3

[0051] Inoculate the mycelium block on the stock culture medium, culture at a constant temperature of 28° C., and obtain the stock boletus for preservation in 20 days, wherein the stock culture medium contains the following components:

[0052]

[0053]

[0054] The color of the obtained black boletus parent species is milky yellow to golden, with sterile skin, sterile strands, no water spit, and good ductility;

[0055] Make the mycelium block of the black boletus parent species with a length and width of 1.1cm×0.4cm, and put it into a sterilized low-temperature freezing tube according to aseptic operation. The specification of the low-temperature freezing tube is 2ml, internal rotation, The low-temperature cryopreservation tube contains 1.5ml of sterile 40% trehalose. Tighten the stopper and seal it with medical tape for two laps. Shake slightly to make the mycelium block completely immersed in the protective agent. The number of bacteria blocks is 3 pieces;

[0056] Th...

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Abstract

The invention relates to the field of strain preservation, and particularly relates to a boletus aereus mother strain long-time preservation method. The boletus aereus mother strain long-time preservation method is characterized in that trehalose is used as a protective agent to preserve a boletus aereus mother strain. According to the boletus aereus mother strain long-time preservation method, the trehalose is used as the protective agent to preserve the boletus aereus mother strain, so that the problem for preserving the boletus aereus mother strain for a long time can be solved; moreover, the method is simple to operate, low in cost, unlikely to degrade and mutate, high in survival rate and good in application prospect. Meanwhile, the method also plays a role in actively promoting the perfection of other high-temperature edible fungi strain preservation technologies.

Description

technical field [0001] The invention relates to the field of strain preservation, in particular to a method for long-term preservation of boletus female species. Background technique [0002] Black boletus (Phlebopus portentosus), a precious tropical edible fungus, is deeply loved by people in the place where it is produced. It is also the only edible fungus in this genus that can be cultivated by saprophytic methods. Currently, it can be cultivated in bags and bottles. Carry out medium-scale cultivation promotion. Black boletus is a kind of high-temperature edible fungus, and mycelium is not resistant to low temperature. Microorganisms and general edible fungus strains are commonly used for low-temperature (4°C) preservation, and the 24-hour mortality rate of preserved black boletus strains is 100%. Therefore, medium and short-term preservation of strains needs to be carried out under medium temperature conditions, and long-term preservation has not been reported. Black b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCC12N1/04
Inventor 纪光燕周茂纪光玉杨太茂杨君巧谢飞
Owner JINGHONG HONGZHEN AGRI TECH CO LTD
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