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Actinomycete strain KN37 and application thereof

A technology of KN37 and actinomycetes, applied in the field of bioengineering, can solve problems such as environmental pollution and restrictions on the export of agricultural products, and achieve a good inhibitory effect

Inactive Publication Date: 2017-02-22
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, the extensive use of chemical pesticides has led to problems such as restrictions on the export of agricultural products, drug resistance, and environmental pollution.

Method used

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  • Actinomycete strain KN37 and application thereof
  • Actinomycete strain KN37 and application thereof
  • Actinomycete strain KN37 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Isolation and identification of bacterial strain KN37

[0029] (1) Actinomycetes strain KN37 is an actinomycetes isolated from the soil collected in Kanas, Xinjiang. The seed culture condition of the bacterial strain KN37 is: under the conditions of 28°C and 120rpm, culture in a medium for 36h on a shaker; the medium consists of: peptone 10g / L, yeast extract 2g / L, acid hydrolyzed casein 2g / L L, sodium chloride 6g / L, pH value 7.4.

[0030] (2) The 16S rDNA gene sequencing method was used to classify and identify the actinomycete strain KN37.

[0031] 16S rDNA sequence determination was entrusted to Shanghai Sangon Bioengineering Co., Ltd. The specific sequencing method is as follows: Genomic DNA was extracted from fresh bacteria by lysozyme method, 16S rDNA was amplified with universal primers (27F and 1492R), and the PCR products were sequenced after detection.

[0032] After determination, the 16S rDNA sequence of the strain KN37 is as follows:

[0033]A...

Embodiment 2

[0041] Embodiment 2: the biological activity and application thereof of bacterial strain KN37

[0042] (1) Inhibitory activity of bacterial strain KN37 to pathogenic bacteria

[0043] The growth rate method was used to determine the effects of strain KN37 fermentation broth on tomato cinerea, tomato early blight, tomato leaf mold, tomato infestation, tomato bacterial spot, cotton Fusarium wilt, cotton Verticillium dahliae, capsicum phytophthora, Apple anthracnose, cucumber anthracnose, rape sclerotinia, wheat head blight, rice sheath blight, cabbage black spot, corn small spot, tobacco red spot, rice blast, root rot, damping off, etc. Inhibitory activity against various pathogenic bacteria. The results are shown in Table 2. The bacterial strain KN37 has a good inhibitory effect on most pathogenic bacteria (Table 2).

[0044] Table 2, the inhibitory effect of bacterial strain KN37 fermentation broth on 18 kinds of pathogenic bacteria

[0045] pathogenic bacteria ...

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Abstract

The invention belongs to the technical field of biology engineering and particularly discloses an actinomycete strain KN37 and an application thereof. Through microscopic observation and 16S rDNA sequence analysis, the condition that the separated actinomycete strain KN37 belongs to Streptomyces is identified, and the preservation number of the actinomycete strain KN37 is CGMCC No.13160. A fermentation broth of the strain KN37 has a better inhibition effect on multiple phytopathogens such as Botrytis cinerea, Alternaria solani, Fusarium oxysporum, Fulvia fulva and the like and can be used for preparing microbial agents for controlling crop diseases.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, relates to microorganisms and applications thereof, in particular to an actinomycete strain KN37 belonging to the genus Streptomyces, and its application in inhibiting various crop pathogenic bacteria. Background technique [0002] Xinjiang's superior natural conditions give it inherent advantages in the production of agricultural products. The planting industry is the main industry in Xinjiang's agriculture, accounting for 76% of the total agricultural output value, and is the main source of increasing agricultural income. The crops grown in Xinjiang mainly include grain, oilseeds, cotton, vegetables, fruits and other economic crops. However, plant diseases seriously threaten the normal production of crops, and almost every crop is harmed by a variety of pathogenic fungi. According to statistics, the annual economic loss caused by plant diseases in the world is as high as 2 trillion U.S...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/06A01N63/02A01P3/00C12R1/465
CPCA01N63/10C12P1/06C12N1/205C12R2001/465
Inventor 张国强杨德松韩小强
Owner SHIHEZI UNIVERSITY
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