43 results about "Ethylene synthesis" patented technology

The invention relates to the technical field of navel orange tree cultivation and physiological regulation and control, and discloses an ethylene regulation and control-based low-temperature dry-water prevention and control method for late-maturing navel oranges before harvest. According to the method, the dry-water rate of the navel oranges in a complex environment is reduced through multi-dimensional accurate regulation and control, and the ethylene inhibitor spraying time is flexibly adjusted according to the climate of a planting field and the fruit development process; the ethylene synthesis can be intervened at the optimal opportunity; the method comprises the following steps: preparing a solution according to a formula, scientifically grouping navel orange trees with consistent growth vigor, accurately spraying inhibitors with two concentrations, ensuring that the agent uniformly acts on fruits and leaves, dynamically adjusting the spraying frequency according to rainfall conditions, avoiding agent loss, and after spraying, performing fertilization and watering in combination with a fruit tree phenological period fertilizer requirement rule and soil moisture content. A good growth environment is created for navel orange trees, the control effect is verified and the scheme is optimized through data detection and record analysis of the system after fruits are picked, and the low water rate of the navel oranges under different severe conditions can be reduced by combining the steps, so that the fruit quality and yield are guaranteed.

The invention discloses an application of interaction of a pear transcription factor PbrDDI2 and a PbrAPX14 gene promoter in regulation and control of maturation and senescence of pear fruits. The nucleotide sequence of the pear transcription factor PbrDDI2 is as shown in SEQ ID NO.1 in a sequence table, and the amino acid sequence of the gene coded protein is as shown in SEQ ID NO.2. The invention discloses the transcription factor PbrDDI2 for regulating and controlling the PbrAPX14 gene expression of the pear fruit for the first time. The invention clarifies an action mechanism of interaction of cis-acting elements (TTTGTCG / CGACAAA) in PbrDDI2 and PbrAPX14 gene promoters in clearing endogenous H2O2 accumulation and further inhibiting synthesis of ethylene so as to inhibit fruit ripening and senescence, and provides a theoretical and practical basis for cultivating anti-senescence high-quality pear varieties.

The invention relates to the technical field of plant genetic engineering, and particularly provides cloning of a GbGELP25D gene of gossypium barbadense and application of the GbGELP25D gene of gossypium barbadense. According to the invention, the GbGELP25D gene with a full length of 1092 bp is cloned from the sea island cotton variety Xinhai No. 7 for the first time, and the gene encodes a secretory lipase protein with a signal peptide and is positioned in an extracellular gap. Through bioinformatics analysis, phylogenetic classification, protein structure modeling and signal peptide function verification, it is clear that the gene belongs to a plant GELP family. Furthermore, a virus-induced gene silencing technology is utilized to prove that the silent GbGELP25D can obviously enhance the resistance of cotton to verticillium wilt, and the mechanism of the silent GbGELP25D is closely related to activation of ethylene synthesis and signal channels and induction of expression of disease-resistant related genes. The resistance gene provided by the invention enriches gene resources of cotton verticillium wilt resistance breeding, and has important theoretical significance and application value.

The invention discloses an MT (melatonin) and SNP (single nucleotide polymorphism) compound preservative as well as preparation and application thereof, and relates to the field of compound preservative films. A nitric oxide donor sodium nitroprusside (SNP); tween-80 (Tween-80) is added into auxiliary components to serve as an emulsifying agent, MT and SNP are dissolved in a proper amount of water and evenly stirred, then Tween-80 is added, and the MT and SNP compound preservative is prepared, according to the MT and SNP compound preservative and preparation and application thereof, MT and SNP are natural or quasi-natural components, compared with chemical preservatives, the MT and SNP have smaller potential harm to the environment and the human body, MT and SNP are used in a compound mode, and therefore the MT and SNP compound preservative has the beneficial effects that the MT and the SNP are more environmentally friendly and more environmentally friendly. MT and SNP cooperate with each other to improve the fresh-keeping effect, the antioxidant effect of MT can reduce oxidative damage in the fruits, SNP promotes endogenous NO generation, can regulate signal transduction in the fruits and further inhibits respiration and ethylene synthesis of the fruits, maturing and aging of the fruits are delayed from different angles through cooperation of MT and SNP, and the fresh-keeping effect of the fruits is improved. Meanwhile, the MT and the NO can cooperatively regulate and control signal transduction in the plant body, and the action time of the NO in the fruit body is prolonged.

The invention is applicable to the technical field of plant genetic engineering, and provides a soybean 1-aminocyclopropane-1-carboxylic acid synthase single enzyme mutant based on multipoint deletion, and the soybean 1-aminocyclopropane-1-carboxylic acid synthase single enzyme activity mutant is # imgabs0 #; the protein sequence of the # imgabs1 # is as shown in SEQ ID NO. 1. A functional site modification technology for a soybean ethylene synthesis key enzyme 1-aminocyclopropane-1-carboxylic acid synthase with double enzyme activity is utilized, and specific proline, serine and asparagine residues are deleted at fixed points, so that the soybean ethylene synthesis key enzyme 1-aminocyclopropane-1-carboxylic acid synthase with double enzyme activity is obtained, and the soybean ethylene synthesis key enzyme 1-aminocyclopropane-1-carboxylic acid synthase with double enzyme activity is obtained. The soybean 1-aminocyclopropane-1-carboxylic acid synthase mutant losing C-S lyase activity provides a novel gene resource for creation of new soybean stress-resistant varieties, and has a wide application prospect and an important economic value in stress-resistant soybean molecular breeding.

The invention discloses a high-temperature treatment method for promoting coloring of bagged peaches after picking and keeping hardness, and belongs to the technical field of treatment of fruits after picking. The method aims to solve the problems of post-harvest color difference caused by omission of a bag picking link and easy softening caused by accelerated synthesis of ethylene in the existing bagged peaches, and avoids the risk of chemical reagent residues. According to the technical scheme, the method comprises the following steps: pre-treating bagged peach fruits which are 70-80% in maturity after harvesting and are free of damage, continuously treating the bagged peach fruits in a constant-temperature environment with the temperature of 30-35 DEG C and the relative humidity of 85-90% for 6-9 days, and finally transferring the bagged peach fruits into a low-temperature warehouse with the temperature of 0-2 DEG C for storage for 7-8 days. The core principle is that ethylene synthesis key enzyme activity is inhibited through specific high temperature, ethylene generation is reduced, accumulation of pigments such as anthocyanin is promoted to improve coloring, cell wall degradation is slowed down to maintain hardness, and quality stability is further maintained through subsequent low-temperature storage. The method adopts pure physical temperature regulation and control, is simple and convenient to operate, low in cost and suitable for large-scale popularization, can synchronously improve the appearance color and texture quality of the bagged peaches after being picked, prolongs the storage period and shelf life, and remarkably improves the commodity value and market competitiveness of the fruits.

The invention discloses a bimodal plasma activation fog fruit fresh-keeping device and method based on ethylene precise regulation and control. The device comprises a treatment cabin, a plasma generation unit and a gas-liquid mixing unit. The plasma generation unit comprises a first discharge module and a second discharge module; the first discharge module discharges in a specific proportion of He / N2 / O2 mixed gas to generate first plasma rich in strong oxidizing active oxygen species, and the first plasma is used for efficiently killing surface pathogenic bacteria and oxidizing and decomposing ethylene gas released by fruits; and the second discharge module adopts low-duty-ratio pulse discharge under inert gas to generate second plasma rich in active nitrogen species, and the second plasma is used for mildly permeating the interior of the fruit skin and inhibiting the activity of the endogenous ethylene synthesis key enzyme. According to the method, ethylene metabolism is accurately regulated and controlled through external elimination and internal suppression bimodals, an ethylene regulation and control closed loop is constructed, the environmental ethylene concentration can be continuously maintained to be 0.1 ppm or below, and fresh keeping of respiration climacteric fruits is achieved.

The invention relates to the technical field of chemical engineering, in particular to a mixed freezing dehydration system and method before vinyl chloride synthesis. The device comprises a hydrogen chloride primary cooler, a hydrogen chloride secondary cooler, a first molecular sieve dryer, a second molecular sieve dryer, an airflow cyclic regeneration device, an acid collecting tank, a mixer, a hydrogen chloride conveying pipeline and an acetylene conveying pipeline. Through combination of graded cooling and parallel molecular sieve deep drying, the water-containing volume fraction of mixed gas is stably controlled within 0.003%, the dehydration effect is remarkably improved, the follow-up catalyst activity and the vinyl chloride synthesis reaction efficiency are guaranteed, the parallel molecular sieve dryer can be automatically switched according to the saturation degree, and the process continuity is guaranteed.

The application belongs to the technical field of organic chemistry, and particularly relates to a synthesis method of tri-substituted ethylene based on an alkyliden transfer strategy. The synthesis method comprises that, in the presence of an alkali or rare earth complex catalyst, a double (hetero) aryl methane or aryl heteroaryl methane and an alpha, beta-unsaturated ketone are subjected to an alkyliden transfer reaction in an organic solvent to form a corresponding tri-substituted ethylene product. The method has the advantages of small catalyst consumption, mild conditions, no additional additives, easy-to-obtain raw materials, strong reaction universality, simple operation, no salt-type waste, and only one organic by-product, i.e., a ketone, which is a widely used chemical raw material and meets the development requirements of green chemistry. In addition, the alpha, beta-unsaturated ketone is a new alkylidenating agent for diaryl methane compounds, is stable, has flexible and changeable structure, has excellent utility in the construction of a multi-substituted olefin compound library, and greatly improves the yield of the tri-substituted ethylene product.

The invention provides a polyvinyl alcohol composite degradable fruit preservative film and a preparation method thereof. The preservative film is prepared from the following components: 2 to 5 weight percent of polyvinyl alcohol matrix, 0.1 to 0.5 weight percent of tea polyphenol, 0.05 to 0.2 weight percent of jasmonic acid methyl ester, 0.3 to 0.6 weight percent of nano silicon dioxide, 1 to 3 weight percent of plasticizer and the balance of deionized water, the preparation method comprises the following steps: (1) dissolving the PVA matrix at 80-100 DEG C; (2) ultrasonically dispersing the plasticizer, silicon dioxide and tea polyphenol at 40-60 DEG C; (3) pre-emulsifying methyl jasmonate by Tween-80, and stirring at a high speed; (4) coating and defoaming; and (5) gradient drying. According to the film, the mechanical property is enhanced through a PVA-silicon dioxide composite structure, free radicals are removed through tea polyphenol, ethylene synthase is inhibited through jasmonic acid methyl ester, slow release synergy of double active ingredients is achieved, the degradation rate in soil within 30 days is larger than or equal to 70%, and the requirements for efficient preservation and environmental protection are met.

The utility model belongs to the technical field of vinyl chloride synthesis equipment, and particularly discloses a vinyl chloride dehydration drying tower exhaust pressure stabilizing device which comprises a vinyl chloride dehydration drying tower, and a vinyl chloride dehydration drying tower exhaust pipeline is arranged on one side of the vinyl chloride dehydration drying tower. The other end of the vinyl chloride dehydration drying tower exhaust pipeline is connected with a surge tank, one side of the surge tank is provided with a surge tank exhaust pipeline, the other end of the surge tank exhaust pipeline is connected with an exhaust tank, one side of the exhaust tank is provided with an exhaust tank exhaust pipeline, and the other end of the exhaust tank exhaust pipeline is connected with a gas holder; the flow stabilizing system and the pressure stabilizing system are arranged between the vinyl chloride dehydration drying tower and the gas holder, so that the stability of the vinyl chloride dehydration drying tower during gas exhausting is realized, the phenomenon that the gas hood is overturned due to overlarge gas flow is avoided, and the device is simple in structure, low in manufacturing cost, convenient to use, easy to maintain and long in service life.

This invention relates to the field of microbiology, specifically to a *Variovorax paradoxus* JKM1 strain that produces ACC deaminase, along with its uses and methods of application. This strain was deposited on October 15, 2024, at the China General Microbiological Culture Collection Center (CGMCC), accession number CGMCC NO: 32218. The *Variovorax paradoxus* JKM1 strain of this invention, which produces ACC deaminase, can be used to alleviate soil compaction stress and promote plant growth. The uses and methods of application of the *Variovorax paradoxus* JKM1 strain of this invention include: degrading the ethylene synthesis precursor 1-aminocyclopropane-1-carboxylic acid (ACC), thereby effectively reducing the ethylene content in the root zone of plants under soil compaction stress and promoting plant growth; increasing the content or synthesis of IAA and ABA in the roots under soil compaction stress; regulating root morphology; and enhancing the plant's tolerance to soil compaction stress.

The invention provides a notopterygium root complex microbial inoculant with excellent shape and high quality and a use method thereof, and belongs to the technical field of plant cultivation. The invention provides a complex microbial inoculant for inhibiting the formation of lateral roots of notopterygium seedlings, which comprises Witzia ciliaris and Brevibacillus laterosporus. The complex microbial inoculant is mainly used for reducing the content of 1-aminocyclopropane-1-carboxylic acid (on the premise of ethylene synthesis) and the content of ICA (Indoleacetic Acid) (auxin) by increasing the content of salicylic acid; the activity of peroxidase and glutamine synthetase and the content of total phenols in plants are improved; the contents of soil urease, polyphenol oxidase and alkali-hydrolyzable nitrogen are increased, and the contents of rapidly available potassium and total phenolic acid are reduced; and ammonium nitrogen is promoted to be converted into nitrate nitrogen, so that the generation of lateral roots of the notopterygium roots is inhibited, the nitrogen content of seedlings of the notopterygium roots is increased, the disease resistance is improved, and the breeding of excellent-shape and high-quality seedlings is promoted.

The utility model provides an anti-pollution vinyl chloride synthesis system which comprises treatment reaction equipment, a hydrogen chloride supply device, an acetylene supply device and a nitrogen supply device, wherein the treatment reaction equipment is used for generating vinyl chloride; the hydrogen chloride supply device comprises a hydrogen chloride storage tank and a gas pipeline, and the gas pipeline is connected with the treatment reaction equipment hydrogen chloride storage tank and the treatment reaction equipment; the acetylene gas supply device is connected with the treatment reaction equipment through a pipeline; and the nitrogen supply device is communicated with the gas conveying pipeline of the treatment reaction equipment. In the utility model, the nitrogen supply device is connected with the gas conveying pipeline of the hydrogen chloride gas supply device, and when the equipment needs to be replaced and maintained, the nitrogen supply device supplies nitrogen into the gas conveying pipeline, so that residual hydrogen chloride gas in the pipeline and the equipment moves towards the treatment reaction equipment and is reacted, thereby ensuring that no hydrogen chloride gas escapes during replacement and maintenance; the working environment safety of personnel is guaranteed, and the situation that the personnel are in gas is avoided.

The invention discloses a tomato histone demethylase SlJMJ7 mutant and application thereof, TGC at the 385th site of a wild type SlJMJ7 gene is mutated into TCA and cysteine Cys at the 385th site is mutated into serine Ser through an overlapping extension three-wheel PCR (Polymerase Chain Reaction) method, the mutant SlJMJ7C385S gene is obtained, the nucleotide sequence of the SlJMJ7C385S gene is as shown in SEQ ID NO: 2, and the amino acid sequence of the SlJMJ7C385S gene is as shown in SEQ ID NO: 4; the inhibition function of SlJMJ7 is specifically relieved through single-point mutation, the pleiotropic risk caused by gene knockout is avoided, the mutant protein can restore the ethylene synthesis and carotenoid accumulation pathway inhibited by wild type SlJMJ7, the maturation process is accelerated, the protein subcellular localization and expression level are not affected by mutation, and the continuity of the regulation effect is ensured. Through site-specific mutagenesis of a C385 site of SlJMJ7, the inhibition effect of the protein on tomato fruit maturity is successfully relieved.

The invention is applicable to the technical field of plant genetic engineering, and provides a soybean ACS double-enzyme inactivation mutant caused by deletion of an asparagine residue and application, the soybean ACS double-enzyme inactivation mutant is GmAH4 delta 209N, and the amino acid sequence of the GmAH4 delta 209N is shown as SEQ ID NO.1. The invention further provides a preparation method of the soybean ACS double-enzyme inactivation mutant. A soybean ACS double-enzyme inactivation mutant in which ACS and C-S lyase activities are completely lost is obtained by utilizing a functional site modification technology of a soybean ethylene synthesis key enzyme ACS and adopting a gene editing means, and the alkali stress tolerance of the obtained soybean gene editing mutant is remarkably enhanced compared with that of wild soybean; a novel gene resource is provided for creation of soybean stress-resistant varieties, and the gene has a wide application prospect and an important economic value in molecular breeding of stress-resistant soybeans.

The utility model belongs to the field of vinyl chloride synthesis, and particularly discloses a vinyl chloride mixed gas cooler unfreezing device which comprises a heating tank, an inner coil pipe is arranged in the heating tank, a hot water feeding pipeline is connected to a water inlet pipeline of the inner coil pipe, a hot water return pipeline is connected to a water outlet pipeline of the inner coil pipe, and a water outlet pipeline of the inner coil pipe is connected with a water inlet pipeline of the hot water return pipeline. One side of the heating tank is connected with a delivery pump through a delivery pump liquid inlet pipeline, the delivery pump is connected with a vinyl chloride mixed gas cooler through a delivery pump liquid outlet pipeline, and the vinyl chloride mixed gas cooler is connected with the heating tank through a vinyl chloride mixed gas cooler liquid outlet pipeline; the brine is heated through the heating tank, then the heated brine is conveyed into the chloroethylene mixed gas cooler through the conveying pump to be unfrozen, and the device is simple in structure, low in manufacturing cost, convenient to use, easy to maintain and long in service life.

The invention provides application of geranyl linalool to inhibition of growth of lateral roots of notopterygium incisum seedlings and / or breeding of notopterygium incisum superior seedlings, and belongs to the technical field of planting of wild notopterygium incisum seedlings. In the invention, the geranyl linalool can promote the elongation of the roots of the notopterygium incisum seedlings and inhibit the formation of lateral roots by increasing the content of auxin in the notopterygium incisum seedlings and reducing the contents of jasmonic acid, abscisic acid and 1-aminocyclopropane-1-carboxylic acid (an ethylene synthesis precursor). Moreover, the geranyl linalool can also improve the activity of polyphenol oxidase and urease in rhizosphere soil, improve the activity of nitrate reductase and glutamine synthetase in leaves, improve the content of alkali-hydrolyzable nitrogen in soil, reduce the content of total phenolic acid in soil and promote conversion of ammonium nitrogen into nitrate nitrogen, so that nitrogen absorption and utilization are promoted, and growth and shape optimization of seedlings are facilitated. In addition, the geranyl linalool can also improve the abundance of rhizosphere soil probiotic bacteria and reduce the abundance of rhizosphere soil pathogenic fungi, so that the disease resistance of the notopterygium incisum seedlings is improved.

The invention discloses an ethylene synthesis method, and belongs to the technical field of organic chemical industry and catalysis. According to the technical scheme, ethanol is in contact with an alkali metal type mordenite catalyst which is not subjected to ion exchange treatment for a gas-phase dehydration reaction, ethylene is generated in a high-selectivity mode, no ion exchange post-treatment step is carried out in the preparation process of the catalyst, the technological process of the prior art is shortened, and the production cost is reduced. And a wastewater treatment step can be omitted.

The invention provides a soybean GmACS18 gene and application of a biological material of the soybean GmACS18 gene to improvement of resistance of crops to soybean phytophthora root rot, and belongs to the technical field of genetic engineering. The biological material for overexpressing the gene comprises an expression cassette for overexpressing the GmACS18 gene, a recombinant vector for overexpressing the GmACS18 gene, a recombinant cell for overexpressing the GmACS18 gene, and a plant tissue for overexpressing the GmACS18 gene. It is found that the soybean GmACS18 gene can improve the defense ability of soybeans to phytophthora root rot by improving the ACC synthetase activity and the content of an ethylene synthesis precursor ACC in the ethylene synthesis process. The discovery provides important gene basis and theoretical support for researching related mechanisms of soybean phytophthora root rot resistance, and has important application value in soybean disease-resistant gene engineering breeding.

The invention discloses a transformer oil composition which is characterized by comprising liquid polyethylene with narrow molecular weight distribution, synthetic aromatic hydrocarbon and an antioxidant, the low-smoke halogen-free flame-retardant polyethylene material is prepared from the following components in parts by weight: 94.7 to 97.85 parts of liquid polyethylene with narrow molecular weight distribution, 2.0 to 5.0 parts of synthetic aromatic diisopropyl naphthalene and 0.15 to 0.3 part of antioxidant. The invention also discloses a preparation method and application thereof. The viscosity of the transformer oil composition at 40 DEG C is smaller than 6.0 mm < 2 > / s, the gassing property is not larger than + 30 mu L / min, the transformer oil composition has extremely low kinematic viscosity, good electrical performance, appropriate gassing resistance and extremely good oxidation resistance, meanwhile, the TVAI specification (high grade) in the IEC60296: 2020 standard and the class II specification index requirements in the ASTMD3487-16 standard are met, and meanwhile, the transformer oil composition has good biodegradability.

This invention discloses the application of the HbCBF1 gene in regulating ethylene content in rubber trees, belonging to the field of agricultural biotechnology. By overexpressing the HbCBF1 gene (nucleotide sequence shown in SEQ ID NO.1), this invention significantly enhances the biosynthesis of ethylene in transgenic plants, manifested as an increased ethylene release rate, elevated content of the ethylene precursor ACC, and enhanced activity of key enzymes in the ethylene synthesis pathway, SAMS, ACS, and ACO. It also exhibits dwarfing phenotypes associated with excessive ethylene accumulation, including significantly reduced plant height, thinner stems, shorter internodes, and accelerated leaf senescence. Molecular mechanism studies show that HbCBF1 directly activates the transcriptional expression of SAMS1 by binding to the CRT / DRE cis-acting element in the SAMS1 gene promoter, thereby upregulating ethylene biosynthesis. This invention provides a new target gene and regulatory method for the genetic engineering improvement of rubber trees.

The invention discloses a fluidized bed activated carbon catalyst in vinyl acetate synthesis reaction and a preparation method thereof, and belongs to the technical field of activated carbon. The active carbon catalyst prepared by the method has the advantages of high catalytic efficiency, long service life and the like, can be operated under relatively high pressure, the prepared vinyl acetate is high in yield and few in by-products, and the content of benzene in the vinyl acetate product can be remarkably reduced.

The application relates to the field of citrus planting technology, in particular to application of exogenous ACC in delaying flowering time of citrus and increasing flowering quantity, and more particularly to a method for delaying flowering time of citrus, increasing flowering quantity, flower formation rate and fruit setting rate, which comprises the following steps: first time spraying exogenous ethylene synthesis precursor ACC on the whole plant before autumn flower bud differentiation of the citrus, and then spraying once every 12-16 days, and spraying for 5-7 times in total. The method can delay the flowering time of the citrus by 3.19 days, and the flowering quantity, flower formation efficiency and fruit setting rate are all improved, thereby providing technical support for solving the influence of extreme weather such as late spring cold on the yield and quality of the citrus.

The invention discloses a thermal coupling system and method for driving polyvinyl chloride drying through vinyl chloride synthesis waste heat, a vinyl chloride synthesis device is coupled with polyvinyl chloride drying equipment, and hot water in the operation process of the vinyl chloride synthesis device is used for preheating cold air in a finned tube air radiator and is introduced into a flash tank; steam generated by the flash tank is used for heating preheated air, a stepped heat supply mode of hot water preheating and steam temperature adjustment is formed, and the hot air temperature of an inlet of a drying tower can be accurately controlled by adjusting the opening degree of a hot air adjusting valve; the waste heat utilization rate is increased, the external supply energy consumption of the drying device is reduced, energy conservation and consumption reduction are achieved, the qualification rate and quality of polyvinyl chloride products are improved, and the equipment investment and the occupied area can be reduced.

The application discloses application of a pear transcription factor PbbHLH164 in promoting fruit ripening, screens a transcription factor PbbHLH164 which is separated from pear fruits and has the function of promoting fruit ripening, and the nucleotide sequence of the transcription factor PbbHLH164 is shown in the sequence table SEQ ID NO.1. The gene is transiently expressed in pear fruits and stably expressed in pear pulp callus through genetic transformation mediated by agrobacterium, and the result shows that the gene expression amount of ethylene synthesis enzyme and the content of ethylene significantly increase after overexpression of PbbHLH164, and the ripening of the fruits is promoted. Through biological function verification, it is shown that the PbbHLH164 gene cloned in the application has the function of promoting ethylene synthesis to participate in fruit ripening. The discovery of the gene provides a new gene resource for molecular breeding of promoting ethylene synthesis, and development and utilization of the resource are favorable to improve the commodity value of pear fruits, prolong the shelf life of the fruits, and are favorable to reduce agricultural cost and realize environment friendliness.

The application provides a nuxvomica optimum form high-quality composite microbial inoculant and a use method, and belongs to the technical field of plant cultivation. The application provides a composite microbial inoculant for inhibiting the formation of lateral roots of nuxvomica seedlings, which comprises Weizmannia coagulans and Brevibacillus parabrevis, and mainly improves the content of salicylic acid, reduces the content of 1-aminocyclopropane-1-carboxylic acid (a precursor of ethylene synthesis) and ICA (an auxin), improves the activities of peroxidase and glutamine synthetase and the content of total phenol of plants, improves the contents of soil urease, polyphenol oxidase and alkaline hydrolyzable nitrogen, reduces the contents of available potassium and total phenolic acid, and promotes the conversion of ammonium nitrogen to nitrate nitrogen, so that the formation of lateral roots of nuxvomica is inhibited, the nitrogen content of the roots of nuxvomica seedlings is improved, the disease resistance is improved, and the breeding of optimum form and high-quality seedlings is promoted.

The high-pressure polyethylene synthesis initiator feeding pump disclosed by the application is provided with two high-pressure hydraulic cylinders at two ends of a low-pressure oil cylinder assembly, and each high-pressure hydraulic cylinder is provided with a valve mounting seat at two ends, and the valve mounting seat is provided with an inlet and an outlet; the low-pressure oil cylinder assembly is provided with a piston assembly, and the piston assembly is movably penetrated into the two high-pressure hydraulic cylinders at two ends of the low-pressure oil cylinder assembly; the two high-pressure hydraulic cylinders are respectively provided with a plunger; the piston assembly is provided with a displacement sensor, the displacement sensor is connected with an electrical control unit, and the low-pressure oil cylinder assembly is communicated with the hydraulic driving unit through hydraulic pipelines at two ends. The high-pressure polyethylene synthesis initiator feeding pump provided by the application solves the problems of unstable flow, low precision and easy leakage of the existing feeding pump when conveying the initiator.

The invention is suitable for the technical field of gene engineering, and provides a soybean ACS-CSL double-enzyme inactivation point mutant, and the soybean ACS-CSL double-enzyme inactivation point mutant comprises GmAH3209L and GmAH4209V. The amino acid sequence of the GmAH3209L is as shown in SEQ ID NO. 1, and the amino acid sequence of the GmAH4209V is as shown in SEQ ID NO. 2. According to the present invention, by using the functional site modification technology of the soybean ethylene synthesis key enzyme 1-aminocyclopropane-1-carboxylic acid synthase, and by inserting the specific base at the fixed point, the double-enzyme inactivation mutant with 1-aminocyclopropane-1-carboxylic acid synthase (ACS) activity and C-S lyase (CSL) activity loss is obtained, such that the novel target and the gene resource are provided for the creation of the soybean stress resistance variety, and the new application prospect is provided. The method has a wide application prospect and an important economic value in stress-resistant soybean molecular breeding.

The invention discloses a tubular reactor for high-pressure polyethylene synthesis, which comprises a reaction tube and is characterized in that the inner wall of the tube body is provided with a continuous spiral groove structure, and the reaction tube is divided into a reaction area and a heat removal area with different functions. In the reaction area, a groove with a triangular arc bottom section and a larger depth and a larger helical angle is adopted to strongly disturb the fluid and strengthen the turbulent mixing and reaction process. And in the heat removal area, a groove with a circular cross section, a smaller depth and a smaller helical angle is adopted, so that a lower flow resistance is maintained while a heat boundary layer is efficiently destroyed to strengthen heat removal. According to the invention, the reaction mixing efficiency and the heat removal efficiency are improved at the same time by virtue of the partitioned optimized groove structure and the active utilization of the vortex principle, and the pipe wall is endowed with excellent self-cleaning capability by virtue of the smooth groove morphology, so that polymer scaling is effectively inhibited, and the operation efficiency and the stability of the reactor are remarkably improved.