26 results about "Ethylene synthesis" patented technology

The invention relates to the technical field of plant genetic engineering, and particularly provides cloning of a GbGELP25D gene of gossypium barbadense and application of the GbGELP25D gene of gossypium barbadense. According to the invention, the GbGELP25D gene with a full length of 1092 bp is cloned from the sea island cotton variety Xinhai No. 7 for the first time, and the gene encodes a secretory lipase protein with a signal peptide and is positioned in an extracellular gap. Through bioinformatics analysis, phylogenetic classification, protein structure modeling and signal peptide function verification, it is clear that the gene belongs to a plant GELP family. Furthermore, a virus-induced gene silencing technology is utilized to prove that the silent GbGELP25D can obviously enhance the resistance of cotton to verticillium wilt, and the mechanism of the silent GbGELP25D is closely related to activation of ethylene synthesis and signal channels and induction of expression of disease-resistant related genes. The resistance gene provided by the invention enriches gene resources of cotton verticillium wilt resistance breeding, and has important theoretical significance and application value.

The invention discloses a high-temperature treatment method for promoting coloring of bagged peaches after picking and keeping hardness, and belongs to the technical field of treatment of fruits after picking. The method aims to solve the problems of post-harvest color difference caused by omission of a bag picking link and easy softening caused by accelerated synthesis of ethylene in the existing bagged peaches, and avoids the risk of chemical reagent residues. According to the technical scheme, the method comprises the following steps: pre-treating bagged peach fruits which are 70-80% in maturity after harvesting and are free of damage, continuously treating the bagged peach fruits in a constant-temperature environment with the temperature of 30-35 DEG C and the relative humidity of 85-90% for 6-9 days, and finally transferring the bagged peach fruits into a low-temperature warehouse with the temperature of 0-2 DEG C for storage for 7-8 days. The core principle is that ethylene synthesis key enzyme activity is inhibited through specific high temperature, ethylene generation is reduced, accumulation of pigments such as anthocyanin is promoted to improve coloring, cell wall degradation is slowed down to maintain hardness, and quality stability is further maintained through subsequent low-temperature storage. The method adopts pure physical temperature regulation and control, is simple and convenient to operate, low in cost and suitable for large-scale popularization, can synchronously improve the appearance color and texture quality of the bagged peaches after being picked, prolongs the storage period and shelf life, and remarkably improves the commodity value and market competitiveness of the fruits.

The invention discloses a bimodal plasma activation fog fruit fresh-keeping device and method based on ethylene precise regulation and control. The device comprises a treatment cabin, a plasma generation unit and a gas-liquid mixing unit. The plasma generation unit comprises a first discharge module and a second discharge module; the first discharge module discharges in a specific proportion of He / N2 / O2 mixed gas to generate first plasma rich in strong oxidizing active oxygen species, and the first plasma is used for efficiently killing surface pathogenic bacteria and oxidizing and decomposing ethylene gas released by fruits; and the second discharge module adopts low-duty-ratio pulse discharge under inert gas to generate second plasma rich in active nitrogen species, and the second plasma is used for mildly permeating the interior of the fruit skin and inhibiting the activity of the endogenous ethylene synthesis key enzyme. According to the method, ethylene metabolism is accurately regulated and controlled through external elimination and internal suppression bimodals, an ethylene regulation and control closed loop is constructed, the environmental ethylene concentration can be continuously maintained to be 0.1 ppm or below, and fresh keeping of respiration climacteric fruits is achieved.

The invention relates to the technical field of chemical engineering, in particular to a mixed freezing dehydration system and method before vinyl chloride synthesis. The device comprises a hydrogen chloride primary cooler, a hydrogen chloride secondary cooler, a first molecular sieve dryer, a second molecular sieve dryer, an airflow cyclic regeneration device, an acid collecting tank, a mixer, a hydrogen chloride conveying pipeline and an acetylene conveying pipeline. Through combination of graded cooling and parallel molecular sieve deep drying, the water-containing volume fraction of mixed gas is stably controlled within 0.003%, the dehydration effect is remarkably improved, the follow-up catalyst activity and the vinyl chloride synthesis reaction efficiency are guaranteed, the parallel molecular sieve dryer can be automatically switched according to the saturation degree, and the process continuity is guaranteed.

This invention relates to the field of microbiology, specifically to a *Variovorax paradoxus* JKM1 strain that produces ACC deaminase, along with its uses and methods of application. This strain was deposited on October 15, 2024, at the China General Microbiological Culture Collection Center (CGMCC), accession number CGMCC NO: 32218. The *Variovorax paradoxus* JKM1 strain of this invention, which produces ACC deaminase, can be used to alleviate soil compaction stress and promote plant growth. The uses and methods of application of the *Variovorax paradoxus* JKM1 strain of this invention include: degrading the ethylene synthesis precursor 1-aminocyclopropane-1-carboxylic acid (ACC), thereby effectively reducing the ethylene content in the root zone of plants under soil compaction stress and promoting plant growth; increasing the content or synthesis of IAA and ABA in the roots under soil compaction stress; regulating root morphology; and enhancing the plant's tolerance to soil compaction stress.

The utility model provides an anti-pollution vinyl chloride synthesis system which comprises treatment reaction equipment, a hydrogen chloride supply device, an acetylene supply device and a nitrogen supply device, wherein the treatment reaction equipment is used for generating vinyl chloride; the hydrogen chloride supply device comprises a hydrogen chloride storage tank and a gas pipeline, and the gas pipeline is connected with the treatment reaction equipment hydrogen chloride storage tank and the treatment reaction equipment; the acetylene gas supply device is connected with the treatment reaction equipment through a pipeline; and the nitrogen supply device is communicated with the gas conveying pipeline of the treatment reaction equipment. In the utility model, the nitrogen supply device is connected with the gas conveying pipeline of the hydrogen chloride gas supply device, and when the equipment needs to be replaced and maintained, the nitrogen supply device supplies nitrogen into the gas conveying pipeline, so that residual hydrogen chloride gas in the pipeline and the equipment moves towards the treatment reaction equipment and is reacted, thereby ensuring that no hydrogen chloride gas escapes during replacement and maintenance; the working environment safety of personnel is guaranteed, and the situation that the personnel are in gas is avoided.

The utility model belongs to the field of vinyl chloride synthesis, and particularly discloses a vinyl chloride mixed gas cooler unfreezing device which comprises a heating tank, an inner coil pipe is arranged in the heating tank, a hot water feeding pipeline is connected to a water inlet pipeline of the inner coil pipe, a hot water return pipeline is connected to a water outlet pipeline of the inner coil pipe, and a water outlet pipeline of the inner coil pipe is connected with a water inlet pipeline of the hot water return pipeline. One side of the heating tank is connected with a delivery pump through a delivery pump liquid inlet pipeline, the delivery pump is connected with a vinyl chloride mixed gas cooler through a delivery pump liquid outlet pipeline, and the vinyl chloride mixed gas cooler is connected with the heating tank through a vinyl chloride mixed gas cooler liquid outlet pipeline; the brine is heated through the heating tank, then the heated brine is conveyed into the chloroethylene mixed gas cooler through the conveying pump to be unfrozen, and the device is simple in structure, low in manufacturing cost, convenient to use, easy to maintain and long in service life.

The invention discloses an ethylene synthesis method, and belongs to the technical field of organic chemical industry and catalysis. According to the technical scheme, ethanol is in contact with an alkali metal type mordenite catalyst which is not subjected to ion exchange treatment for a gas-phase dehydration reaction, ethylene is generated in a high-selectivity mode, no ion exchange post-treatment step is carried out in the preparation process of the catalyst, the technological process of the prior art is shortened, and the production cost is reduced. And a wastewater treatment step can be omitted.

The invention provides a soybean GmACS18 gene and application of a biological material of the soybean GmACS18 gene to improvement of resistance of crops to soybean phytophthora root rot, and belongs to the technical field of genetic engineering. The biological material for overexpressing the gene comprises an expression cassette for overexpressing the GmACS18 gene, a recombinant vector for overexpressing the GmACS18 gene, a recombinant cell for overexpressing the GmACS18 gene, and a plant tissue for overexpressing the GmACS18 gene. It is found that the soybean GmACS18 gene can improve the defense ability of soybeans to phytophthora root rot by improving the ACC synthetase activity and the content of an ethylene synthesis precursor ACC in the ethylene synthesis process. The discovery provides important gene basis and theoretical support for researching related mechanisms of soybean phytophthora root rot resistance, and has important application value in soybean disease-resistant gene engineering breeding.

The invention discloses a transformer oil composition which is characterized by comprising liquid polyethylene with narrow molecular weight distribution, synthetic aromatic hydrocarbon and an antioxidant, the low-smoke halogen-free flame-retardant polyethylene material is prepared from the following components in parts by weight: 94.7 to 97.85 parts of liquid polyethylene with narrow molecular weight distribution, 2.0 to 5.0 parts of synthetic aromatic diisopropyl naphthalene and 0.15 to 0.3 part of antioxidant. The invention also discloses a preparation method and application thereof. The viscosity of the transformer oil composition at 40 DEG C is smaller than 6.0 mm < 2 > / s, the gassing property is not larger than + 30 mu L / min, the transformer oil composition has extremely low kinematic viscosity, good electrical performance, appropriate gassing resistance and extremely good oxidation resistance, meanwhile, the TVAI specification (high grade) in the IEC60296: 2020 standard and the class II specification index requirements in the ASTMD3487-16 standard are met, and meanwhile, the transformer oil composition has good biodegradability.

This invention discloses the application of the HbCBF1 gene in regulating ethylene content in rubber trees, belonging to the field of agricultural biotechnology. By overexpressing the HbCBF1 gene (nucleotide sequence shown in SEQ ID NO.1), this invention significantly enhances the biosynthesis of ethylene in transgenic plants, manifested as an increased ethylene release rate, elevated content of the ethylene precursor ACC, and enhanced activity of key enzymes in the ethylene synthesis pathway, SAMS, ACS, and ACO. It also exhibits dwarfing phenotypes associated with excessive ethylene accumulation, including significantly reduced plant height, thinner stems, shorter internodes, and accelerated leaf senescence. Molecular mechanism studies show that HbCBF1 directly activates the transcriptional expression of SAMS1 by binding to the CRT / DRE cis-acting element in the SAMS1 gene promoter, thereby upregulating ethylene biosynthesis. This invention provides a new target gene and regulatory method for the genetic engineering improvement of rubber trees.

The application relates to the field of citrus planting technology, in particular to application of exogenous ACC in delaying flowering time of citrus and increasing flowering quantity, and more particularly to a method for delaying flowering time of citrus, increasing flowering quantity, flower formation rate and fruit setting rate, which comprises the following steps: first time spraying exogenous ethylene synthesis precursor ACC on the whole plant before autumn flower bud differentiation of the citrus, and then spraying once every 12-16 days, and spraying for 5-7 times in total. The method can delay the flowering time of the citrus by 3.19 days, and the flowering quantity, flower formation efficiency and fruit setting rate are all improved, thereby providing technical support for solving the influence of extreme weather such as late spring cold on the yield and quality of the citrus.

The invention discloses a thermal coupling system and method for driving polyvinyl chloride drying through vinyl chloride synthesis waste heat, a vinyl chloride synthesis device is coupled with polyvinyl chloride drying equipment, and hot water in the operation process of the vinyl chloride synthesis device is used for preheating cold air in a finned tube air radiator and is introduced into a flash tank; steam generated by the flash tank is used for heating preheated air, a stepped heat supply mode of hot water preheating and steam temperature adjustment is formed, and the hot air temperature of an inlet of a drying tower can be accurately controlled by adjusting the opening degree of a hot air adjusting valve; the waste heat utilization rate is increased, the external supply energy consumption of the drying device is reduced, energy conservation and consumption reduction are achieved, the qualification rate and quality of polyvinyl chloride products are improved, and the equipment investment and the occupied area can be reduced.

The application discloses application of a pear transcription factor PbbHLH164 in promoting fruit ripening, screens a transcription factor PbbHLH164 which is separated from pear fruits and has the function of promoting fruit ripening, and the nucleotide sequence of the transcription factor PbbHLH164 is shown in the sequence table SEQ ID NO.1. The gene is transiently expressed in pear fruits and stably expressed in pear pulp callus through genetic transformation mediated by agrobacterium, and the result shows that the gene expression amount of ethylene synthesis enzyme and the content of ethylene significantly increase after overexpression of PbbHLH164, and the ripening of the fruits is promoted. Through biological function verification, it is shown that the PbbHLH164 gene cloned in the application has the function of promoting ethylene synthesis to participate in fruit ripening. The discovery of the gene provides a new gene resource for molecular breeding of promoting ethylene synthesis, and development and utilization of the resource are favorable to improve the commodity value of pear fruits, prolong the shelf life of the fruits, and are favorable to reduce agricultural cost and realize environment friendliness.

The application provides a nuxvomica optimum form high-quality composite microbial inoculant and a use method, and belongs to the technical field of plant cultivation. The application provides a composite microbial inoculant for inhibiting the formation of lateral roots of nuxvomica seedlings, which comprises Weizmannia coagulans and Brevibacillus parabrevis, and mainly improves the content of salicylic acid, reduces the content of 1-aminocyclopropane-1-carboxylic acid (a precursor of ethylene synthesis) and ICA (an auxin), improves the activities of peroxidase and glutamine synthetase and the content of total phenol of plants, improves the contents of soil urease, polyphenol oxidase and alkaline hydrolyzable nitrogen, reduces the contents of available potassium and total phenolic acid, and promotes the conversion of ammonium nitrogen to nitrate nitrogen, so that the formation of lateral roots of nuxvomica is inhibited, the nitrogen content of the roots of nuxvomica seedlings is improved, the disease resistance is improved, and the breeding of optimum form and high-quality seedlings is promoted.

The high-pressure polyethylene synthesis initiator feeding pump disclosed by the application is provided with two high-pressure hydraulic cylinders at two ends of a low-pressure oil cylinder assembly, and each high-pressure hydraulic cylinder is provided with a valve mounting seat at two ends, and the valve mounting seat is provided with an inlet and an outlet; the low-pressure oil cylinder assembly is provided with a piston assembly, and the piston assembly is movably penetrated into the two high-pressure hydraulic cylinders at two ends of the low-pressure oil cylinder assembly; the two high-pressure hydraulic cylinders are respectively provided with a plunger; the piston assembly is provided with a displacement sensor, the displacement sensor is connected with an electrical control unit, and the low-pressure oil cylinder assembly is communicated with the hydraulic driving unit through hydraulic pipelines at two ends. The high-pressure polyethylene synthesis initiator feeding pump provided by the application solves the problems of unstable flow, low precision and easy leakage of the existing feeding pump when conveying the initiator.

The invention is suitable for the technical field of gene engineering, and provides a soybean ACS-CSL double-enzyme inactivation point mutant, and the soybean ACS-CSL double-enzyme inactivation point mutant comprises GmAH3209L and GmAH4209V. The amino acid sequence of the GmAH3209L is as shown in SEQ ID NO. 1, and the amino acid sequence of the GmAH4209V is as shown in SEQ ID NO. 2. According to the present invention, by using the functional site modification technology of the soybean ethylene synthesis key enzyme 1-aminocyclopropane-1-carboxylic acid synthase, and by inserting the specific base at the fixed point, the double-enzyme inactivation mutant with 1-aminocyclopropane-1-carboxylic acid synthase (ACS) activity and C-S lyase (CSL) activity loss is obtained, such that the novel target and the gene resource are provided for the creation of the soybean stress resistance variety, and the new application prospect is provided. The method has a wide application prospect and an important economic value in stress-resistant soybean molecular breeding.

The invention discloses a tubular reactor for high-pressure polyethylene synthesis, which comprises a reaction tube and is characterized in that the inner wall of the tube body is provided with a continuous spiral groove structure, and the reaction tube is divided into a reaction area and a heat removal area with different functions. In the reaction area, a groove with a triangular arc bottom section and a larger depth and a larger helical angle is adopted to strongly disturb the fluid and strengthen the turbulent mixing and reaction process. And in the heat removal area, a groove with a circular cross section, a smaller depth and a smaller helical angle is adopted, so that a lower flow resistance is maintained while a heat boundary layer is efficiently destroyed to strengthen heat removal. According to the invention, the reaction mixing efficiency and the heat removal efficiency are improved at the same time by virtue of the partitioned optimized groove structure and the active utilization of the vortex principle, and the pipe wall is endowed with excellent self-cleaning capability by virtue of the smooth groove morphology, so that polymer scaling is effectively inhibited, and the operation efficiency and the stability of the reactor are remarkably improved.

This application discloses a device for recovering and deeply purifying vinyl chloride synthesis displacement gas, specifically relating to the field of chemical gas treatment technology. It includes two alkaline scrubbing towers connected in series, with each tower's outlet equipped with a gas-liquid separator. The end pipes of the two series-connected alkaline scrubbing towers are connected to a raw material gas buffer tank. The outlet of the raw material gas buffer tank is connected to an adsorption tower. The outlet of the adsorption tower's desorbed gas is connected to a vacuum pump. The top crude purified gas outlet of the adsorption tower is connected to a purified gas buffer tank. The outlet of the purified gas buffer tank is connected to a heater. A circulating fan for pressurization is installed between the purified gas buffer tank and the heater. The heater's outlet is connected to two dehydrocarbonization towers connected in series. The end pipes of the two series-connected dehydrocarbonization towers are connected to a cooler. Furthermore, the cooler and the adjacent dehydrocarbonization tower are connected by a circulating pipe leading to the inlet of the circulating fan. This application reduces pipeline corrosion and successfully recovers gases such as vinyl chloride and acetylene.

The invention discloses a preparation method of vinyl acetate synthesis catalyst carrier activated carbon, and relates to the technical field of activated carbon preparation. Comprising the following steps: carrying out crushing and washing treatment on a carbon-containing raw material with ash content of less than 5%; mixing with a certain proportion of oxidant solution, and standing for 6-24 hours; and grinding into powder with the particle size of less than 200-300 meshes, and then adding a binder and a catalytic promoter. The catalyst carrier activated carbon provided by the invention has more developed pore channels through which synthesis raw materials and product molecules can freely enter and exit, the strength and apparent density are higher, and the prepared catalyst carrier activated carbon is detected by adopting an ASAP2020 type full-automatic pore size distribution tester static liquid nitrogen adsorption method; the mesopore volume of the activated carbon with the pore diameter in the range of 1.5-5.0 nm is greater than 0.55 mL / g, and the total pore volume is greater than 1.0 mL / g; the filling density of the activated carbon is greater than 0.45 g / mL, and the abrasive resistance is greater than or equal to 96%.

The utility model provides a vinyl chloride synthetic catalyst dipping device, including dipping tank, medicament introduction mechanism and air extraction mechanism, the dipping tank forms closed dipping space, medicament introduction mechanism is communicated with dipping tank and is used for introducing dipping medicament into the dipping space, the air extraction mechanism is communicated with dipping tank and is used for introducing dipping medicament into the dipping space, and the dipping tank is communicated with the medicament introduction mechanism and is used for introducing the dipping medicament into the dipping space. The air extracting device is used for extracting air in the dipping space; according to the utility model, the air exhaust mechanism is arranged, so that air in the impregnating tank can be exhausted; during impregnation, activated carbon is firstly poured into the impregnation tank and sealed, then air in the impregnation tank is extracted by the air extraction mechanism, so that air in the impregnation tank and the activated carbon is extracted, then the medicament is introduced into the impregnation tank by the medicament introduction mechanism, and the activated carbon is impregnated. According to the utility model, air in pores of activated carbon is effectively removed, and the problem that the air affects the impregnation effect is solved.

The application discloses a fluidized bed activated carbon catalyst in a vinyl acetate synthesis reaction and a preparation method thereof, and belongs to the technical field of activated carbon. The prepared activated carbon catalyst has the advantages of high catalytic efficiency, long service life, and the like, can operate under high pressure, and has the advantages of high vinyl acetate yield, few by-products, and the like, and can significantly reduce the content of benzene in the vinyl acetate product.

The invention belongs to the technical field of food biology, and particularly relates to a dsRNA fragment for prolonging the shelf life of bananas and a composition and application of the dsRNA fragment. The invention firstly provides a dsRNA fragment for reducing ethylene synthesis of bananas and prolonging the shelf life of bananas, particularly a dsRNA1 fragment of a targeted banana gene ACS1, and the corresponding DNA sequence of the dsRNA1 fragment is as shown in SEQ ID NO.3; and / or a dsRNA2 fragment of a targeted banana gene ACO1, and a DNA sequence corresponding to the dsRNA2 fragment is as shown in SEQ ID NO.4. The dsRNA is applied to bananas through spraying or soaking, and key gene expression on an ethylene synthesis pathway can be interfered in a targeted manner, so that ethylene synthesis is inhibited. The shelf life of bananas soaked with the dsRNAs and the dsRNAs composition is remarkably prolonged, and the dsRNAs and the dsRNAs composition have remarkable technical innovation and huge application value and can serve as a main component to be used for development of novel biological fresh-keeping agents.

The application discloses a shape-selective alkylation catalyst and a preparation method and application thereof, and relates to the field of chemical industry. The in-situ doped Mg-ZSM-5 molecular sieve crystal with multistage pores is obtained by an in-situ hydrothermal synthesis method, Mg dispersion and fixation are promoted while the acidity of ZSM-5 is adjusted, and the migration change of Mg in the shape-selective alkylation reaction process is effectively inhibited. The molecular sieve crystal is used as a precursor, neutral transparent nanometer silicon sol is used for bonding and forming, and then secondary crystallization is carried out through the in-situ gas-solid phase crystallization conversion method, so that the nanometer silicon sol completely covered on the surface of the Mg-ZSM-5 is in-situ converted into full-silicon S-1 molecular sieve crystal phase with the same topological structure as the ZSM-5 molecular sieve, the acid sites on the outer surface of the molecular sieve are effectively and completely passivated through the complete coverage of the full-silicon S-1 molecular sieve on the outer surface of the Mg-ZSM-5, and the good connectivity of the molecular sieve channel is reserved. The shape-selective alkylation catalyst can be applied to the alkylation process of synthesizing p-methyl ethyl benzene from toluene and ethanol or ethylene.

The utility model provides a vinyl chloride synthetic gas drying device which comprises a first-stage drying module and a second-stage drying module, the first-stage drying module is used for drying gas, the gas inlet end of the second-stage drying module is connected with the gas outlet end of the first-stage drying module through a pipeline, and the gas inlet end of the second-stage drying module is connected with the gas outlet end of the second-stage drying module through a pipeline. The second-stage drying module is filled with a drying substance which changes color when encountering water and can dry air and detect whether the air is dry or not; the second-stage drying module is additionally arranged at the rear end of the first-stage drying module, the second-stage drying module is filled with the drying matter which changes color when encountering water, and when the first-stage drying module cannot dry gas completely due to the fact that the efficiency of the drying matter in the first-stage drying module is reduced, the second-stage drying module can adsorb residual water in the gas, and therefore the drying efficiency of the gas is improved. When the first-stage drying module fails, the first-stage drying module changes color to prompt that the drying capacity of the drying substance in the first-stage drying module is reduced and the drying substance needs to be replaced, so that the problem that whether the first-stage drying module fails or not cannot be confirmed in time in the prior art can be effectively solved.

The invention discloses an ethylene synthase TaACO3 gene haplotype molecular marker related to wheat powdery mildew resistance and application of the ethylene synthase TaACO3 gene haplotype molecular marker, and belongs to the technical field of molecular marker-assisted breeding. Specifically, the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, and a G / C polymorphic site exists at the 73bp position of the sequence. The invention also develops a primer for detecting the haplotype molecular marker and prepares a detection product containing the primer, and the molecular marker site and genotype can be accurately identified by using the primer, so that the primer is used for screening and identifying wheat powdery mildew resistant varieties, and an important technical means is provided for improving the wheat breeding efficiency.