Small molecular inhibitor pyrazinamide used in plant ethylene synthesis and application thereof

A pyrazinamide agent and a synthesis route technology, which is applied in the field of small molecule inhibitor pyrazinamide, can solve the problems of high price, environmental hazards, non-specific action sites, etc., and achieves easy popularization and application, reduction of synthesis amount, and inhibition effect. strong effect

Active Publication Date: 2014-01-22
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditionally used ethylene inhibitors often have non-specific sites of action. For example, the commonly used ethylene inhibitor AVG is also an auxin synthesis inhibitor and is expensive; and the commonly used silver ion inhibitors are not only non-specific for the site of action, but also harmful to the enviro

Method used

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  • Small molecular inhibitor pyrazinamide used in plant ethylene synthesis and application thereof
  • Small molecular inhibitor pyrazinamide used in plant ethylene synthesis and application thereof
  • Small molecular inhibitor pyrazinamide used in plant ethylene synthesis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Screening of Small Molecule Inhibitors that Inhibit Plant Ethylene Synthesis Pathway

[0018] Using chemical genetics, the small molecule inhibitor screening method, to screen 2,000 small chemical molecules selected from the microsource spectrum database (SP2000, http: / / www.msdiscovery.com) provided by the University of California, Riverside. All the small molecules in the library were made into a stock solution with a concentration of 0.5 mg / 100 μL in DMSO. Related small molecule information can be downloaded from (http: / / www.msdiscovery.com / downloads.html). The structures, molecular formulas, molecular weights, names and functions of the small molecules in the screening library are known, and the concentration of the small molecules in the screening test is 50-100 μM. By screening the library containing 2000 small molecules (gifted by Yang Zhenbiao, University of California, Riverside) (reference: Girke T, Cheng LC, et al. (2005). "ChemMine. A compound mini...

Embodiment 2

[0019] Embodiment 2 The present invention inhibits the medium of plant ethylene synthesis pathway

[0020] The MS medium for inhibiting plant ethylene synthesis pathway of the present invention is prepared by using the small molecule inhibitor pyrazinamide (PZA for short), that is, the MS medium with a concentration of pyrazinamide of 30-50 μM (micromoles per liter) is prepared. The control is commonly used MS medium.

[0021] The experimental materials were Arabidopsis thaliana ethylene-oversynthesis mutant strain eto1-2, and Arabidopsis wild-type Col-0 with normal expression of ethylene.

[0022] Culture eto1-2 and Col-0 with MS medium that inhibits plant ethylene synthesis pathway (that is, MS medium with pyrazinamide added) and control MS medium, and culture them in a 22° incubator for 3 days in the dark and dark .

[0023] Observation after 3 days of cultivation, the results are shown in figure 2 ( figure 2 The effect of adding the small molecule inhibitor pyrazinam...

Embodiment 3

[0024] Embodiment 3 Determination of pyrazinamide action target

[0025] In order to determine the action target of the small-molecule inhibitor pyrazinamide of the present invention, the action of the small-molecule inhibitor was observed after applying excess ethylene synthesis precursor ACC. If the inhibitor acts on the ethylene synthesis pathway, in the presence of excess ethylene synthesis precursor ACC, the small molecule inhibitor pyrazinamide will no longer have a significant inhibitory effect on ethylene synthesis; but if the inhibitor target is located downstream of the ethylene synthesis pathway ethylene signal transduction pathway, in the presence of excess ethylene synthesis precursor ACC, the inhibitory effect of pyrazinamide on ethylene will not be affected, that is, excess ethylene synthesis precursor ACC cannot affect the downstream ethylene signal pathway inhibitors on ethylene Inhibition of the signal.

[0026] The experimental method was referred to Exampl...

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Abstract

The invention discloses a small molecular inhibitor pyrazinamide (shortened as PZA) used in plant ethylene synthesis. The small molecular inhibitor pyrazinamide is characterized by being a specific inhibitor of ACC oxidase (ACO), and being capable of inhibiting conversion of an ethylene synthesis precursor ACC into ethylene in the plant ethylene synthesis; and the pyrazinamide has a structural formula shown as a figure in the abstract, and has the molecular weight of 123.12. The invention further provides a culture medium which contains the pyrazinamide and can inhibit the plant ethylene synthesis. The invention further provides application of the small molecular inhibitor pyrazinamide used in the plant ethylene synthesis to plant growth and development regulation and refreshment during plant transportation. The inhibitor pyrazinamide can obviously inhibit the plant ethylene synthesis, has a stronger effect than known ethylene synthesis inhibitors, and is convenient in popularization and application.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to pyrazinamide, a small molecule inhibitor of plant ethylene synthesis pathway, and its application. Background technique [0002] The plant hormone ethylene plays an important role in plant growth and development and resistance to external biotic and abiotic stresses. For example, ethylene regulates seed germination, leaf senescence, flower abscission, fruit ripening, and unisexual flower sex determination and resistance to external stress. Among them, ethylene promotes the characteristics of fruit ripening and organ shedding, and plays an important role in agricultural production. For example, ethylene is the main active ingredient of fruit ripening agents commonly used in agricultural production. In addition, due to the property of ethylene to promote the aging and shedding of organs, the annual loss of vegetables, fruits and flowers due to aging and corruption caused by ethylene i...

Claims

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Application Information

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IPC IPC(8): C07D241/24A01G31/00A01P21/00A01N3/00
CPCA01N3/00A01N43/60C07D241/24
Inventor 郭红卫何文容
Owner PEKING UNIV
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