Method for in vitro regeneration of Feizixiao litchi variety

Abstract

The invention discloses a method for in vitro regeneration of a Feizixiao litchi variety. The method comprises the following steps: step 1, taking the anther of the Feizixiao litchi variety as an explant for callus induction and culture, and screening to obtain embryogenic callus; the culture medium is added with 2, 4-D with final concentration of 3 mg / l, BA with final concentration of 0.5 mg / l, NAA with final concentration of 0.5 mg / l and sugar with final concentration of 30 g / L on the basis of a culture medium MS; step 2, carrying out somatic embryo induction and culture to the embryogenic callus to obtain a somatic embryo; the culture medium is added with NAA with final concentration of 0.1 mg / l, LKT with final concentration of 5 mg / l, inositol with final concentration of 100 mg / l and sucrose with final concentration of 50 g / L on the basis of the culture medium MS; step 3, carrying out maturation to the somatic embryo to obtain a mature embryo, culture mediums are MS, 50 ml / L coconut milk and 60 g / L sucrose; and step 4, carrying out regeneration culture to the mature embryo, the culture medium is added with sucrose with final concentration of 30 g / L and agar with final concentration of 7 g / L on the culture medium MS. The invention establishes a method for in vitro plant regeneration of a Feizixiao litchi variety, achieves the high induction rate, improves the production efficiency, reduces the cost and lays a good foundation for litchi variety improvement and biotechnology breeding.

Description

technical field [0001] The present invention relates to a method for in vitro regeneration of 'Feizixiao' litchi variety. Specifically, the present invention relates to a culture method for 'Feizixiao' litchi variety induced from callus through somatic embryogenesis to develop into a complete in vitro regenerated plant. Background technique [0002] Litchi (Litchi chinensis Sonn.) is a major tropical and subtropical fruit tree in southern my country. It plays an important role in tropical agricultural production and is an important part of farmers' income. With the improvement of people's living standards, the pursuit of commodity value is getting higher and higher, and the need to speed up litchi breeding technology and new varieties is imminent. Litchi has a long childhood and highly heterozygous genetic composition. Traditional breeding techniques to improve litchi traits and breed new varieties are time-consuming, laborious, and difficult, and can no longer meet the need...

AI Technical Summary

Problems solved by technology

[0003] Since Fu Lianfang et al. reported that litchi tissue culture obtained complete plants in 1983, researchers have carried out tissue culture research one after another, but the progress is relatively slow

At present, the bottleneck of litchi biotechnology breeding lies in the failure to establish an efficient genetic transformation system, and the establishment of an in vitro regeneration system for litchi is the premise and basis for the establishment of an efficient genetic transformation system

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