Piriformospora indica fermentation method and response surface optimization method used by same

A technology of Pyromorpha indica and fermentation method, which is applied in the field of secondary metabolite fermentation production process optimization, can solve problems such as the determination of the composition and structure of secondary metabolites, the unfavorable industrial production of endophytic fungi and agricultural application, etc., to achieve The effect of improving fermentation production yield, high use value and reducing energy consumption

Inactive Publication Date: 2013-09-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Due to the lack of research on Pyromorpha indica, there are different reports on the culture conditions of the fungus in the literature, and the definite composition and structure of its secondary metabolites are still uncertain. Conditions and a unified standard for the production of secondary metabolites by large-scale fermentation, which is not conducive to the large-scale industrial production and agricultural application of this endophytic fungus

Method used

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  • Piriformospora indica fermentation method and response surface optimization method used by same
  • Piriformospora indica fermentation method and response surface optimization method used by same
  • Piriformospora indica fermentation method and response surface optimization method used by same

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Embodiment 1

[0056] Embodiment 1, a kind of Indian piriformis fermentation optimization method comprises the steps:

[0057] Inoculate Pyromorpha indica on PDA plates and activate at 28°C for 14 days. Use a 5mm hole puncher to punch holes on the edge of the activated Piriformis indica flat plate.

[0058] Adjust 300 mL of PDB medium (stock solution) to pH = 5 with 1 mol / L HCl solution, then put the 5 round cakes obtained by punching holes and incubate at 28°C for 14 days.

[0059] Filtrate the piriformis indica fermentation liquid obtained by culturing for 14 days with gauze, mix the filtrate with 50 ml of ethyl acetate in a 1 L separatory funnel, leave it for 2 h, separate the organic phase and the aqueous phase, and extract a total of 3 times; Dry the organic phase with anhydrous sodium sulfate, then concentrate and evaporate to dryness at a temperature of 40-41°C, then dissolve it in 10 mL of absolute ethanol, dilute 100 times with absolute ethanol, take 1 mL of the diluted solution an...

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Abstract

The invention discloses a piriformospora indica fermentation method. The method comprises the following steps: by taking a PDB culture medium as a culture solution, regulating the pH of the culture solution to be 5.0-5.1 by using a HCl solution with the concentration of 1mol/L, and performing culture fermentation on piriformospora indica at the temperature of 27.5-28.5 DEG C for 14-15 days, so as to obtain the fermentation liquor. The invention also discloses a method for performing response surface optimization in the piriformospora indica fermentation process. The method comprises the following steps: setting the three variables including the pH, the concentration of the PDB culture medium and the culture time; optimizing according to the Box-Behnken design principle by utilizing design expert software. According to the method, the yield of secondary metabolites can be increased, and the consumption of nutritional ingredients and culture media is reduced.

Description

technical field [0001] The invention relates to a technique for optimizing the cultivation of Pyromorpha indica fungi and the fermentation production process of secondary metabolites by applying the response surface method. Background technique [0002] Arbuscular mycorrhizal (AM) fungi can form a symbiotic relationship with terrestrial plants, promote nutrient absorption by plant roots, improve plant resistance to diseases and insect pests, barrenness, drought and other adversities, and increase plant biomass to a certain extent . Common AM fungi can only survive on living plants, but the recently discovered plant root endophytic fungus Piriformospora indica can grow on artificial media and successfully complete its life cycle. Studies have found that Pyriformis indica can improve the resistance of plants to high salinity, drought and other adversities, promote the absorption of nutrients in the soil by plants, and also effectively enhance the resistance of plants to vario...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12R1/645
Inventor 陈绍瑗陈煜哲
Owner ZHEJIANG UNIV
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