Aeribacillus pallidus Jh-7 antagonizing xanthomonas campestris pv. oryzae

A rice bacterial bacillus, bacillus technology, applied in the direction of bacteria, microorganism-based methods, chemicals for biological control, etc., can solve problems such as pollution of the environment, human health hazards, yield loss, etc.

Active Publication Date: 2018-09-14
ZHEJIANG NORMAL UNIVERSITY
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  • Abstract
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Problems solved by technology

Once rice is infected with the pathogen, it will cause 20%-40% yield loss
As the main control measures of rice diseases, chemical control is simple and efficient, but it also has many disadvantages. The extensive use of chemical pesticides not only causes great harm to human health, but also seriously pollutes the environment. Long-term use will also lead to drug resistance of pathogenic bacteria

Method used

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  • Aeribacillus pallidus Jh-7 antagonizing xanthomonas campestris pv. oryzae
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  • Aeribacillus pallidus Jh-7 antagonizing xanthomonas campestris pv. oryzae

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Embodiment Construction

[0023] Materials and Methods

[0024] 1.1 Tested bacteria

[0025] The biocontrol bacteria to be tested: Aerobacillus pale (Jh-7), isolated from the soil of Zhejiang Normal University campus by itself, and stored in a -80 ℃ refrigerator in our laboratory.

[0026] Pathogens tested: Rice Leaf Blight ( Xanthomonas campestris pv. oryzae ) is Philippine race 6 (PR6), provided by our laboratory and stored in a -80°C refrigerator.

[0027] culture medium

[0028] LB medium (tryptone 10 g, yeast extract 5 g, sodium chloride 10 g, agar 15-20 g, distilled water 1 000 mL, natural pH, autoclaved at 121 °C for 20 min).

[0029] NA medium (tryptone 5 g, yeast extract 1 g, sucrose 10 g, beef extract 3 g, agar 10 g, distilled water 1000 mL, natural pH, autoclaved at 121 °C for 20 min).

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Abstract

The invention relates to aeribacillus pallidus Jh-7 antagonizing xanthomonas campestris pv. oryzae. The invention aims at providing the aeribacillus pallidus Jh-7 antagonizing the xanthomonas campestris pv. oryzae firstly. The aeribacillus pallidus contains an ingredient for inhibiting the xanthomonas campestris pv. oryzae, and a good approach is provided for biological control of rice diseases caused by the xanthomonas campestris pv. oryzae. The invention aims at providing a metabolism crude extract of the aeribacillus pallidus Jh-7 secondly. The invention aims at providing an application ofthe metabolism crude extract of the aeribacillus pallidus Jh-7 thirdly. The invention aims at providing a biological pesticide containing the aeribacillus pallidus Jh-7 fourthly.

Description

technical field [0001] The invention relates to a strain of Aerobacillus pallidum antagonizing rice bacterial blight. Background technique [0002] Microorganisms can produce a large amount of active substances, and have some advantages that chemical synthesis cannot achieve, such as high activity, wide range of effects, and good safety. At the same time, microbial resources have the characteristics of rapid growth, easy cultivation and easy production and amplification. Therefore, microorganisms have become an important research object for researching lead compounds. Among microorganisms, Bacillus is widely studied and applied, and it is a rod-shaped bacterium that can produce endospores with stress resistance. According to reports, most Bacillus species can produce active substances, and there are many kinds and strong activities. At present, nearly 800 biologically active substances have been purified and identified in Bacillus, which are mainly divided into two catego...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00C12R1/01
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/01
Inventor 孙梅好周宏飞张晓张君辛德东蒲首丞巩菊芳
Owner ZHEJIANG NORMAL UNIVERSITY
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