Preparation method of selenium-rich yeast

A technology of selenium-enriched yeast and yeast, which is applied in the field of fermentation industry, can solve the problems such as the optimization of selenium-enriched yeast without selenium-enriched yeast, and achieve the effect of enhancing selenium-enriching ability, strong selenium-enriching ability, and high biomass

Inactive Publication Date: 2018-04-03
常州禾吉纺织品有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Technical problem to be solved by the present invention: Aiming at the problem that there is no screening and breeding of yeast strains at present, a yeast strain is randomly used, and

Method used

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Examples

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preparation example Construction

[0032] A preparation method of selenium-enriched yeast, comprising the steps of:

[0033] (1) Add soil samples from Bama County, Guangxi, into yeast liquid culture medium at a mass ratio of 4:9, and culture on a shaking table at 200 r / min at 30-35°C for 18-24 hours to obtain yeast culture medium. Fraction of 0.9% normal saline was serially diluted to 10 -6 Dilution level, get the diluted yeast culture solution, spread the yeast culture solution on the yeast plate medium, cultivate in a constant temperature incubator at 30~35°C for 36~48h, pick the single yeast colony with the largest diameter Streak the plate 2-3 times to obtain a single colony of purified yeast;

[0034] (2) Pick a single colony of the above-mentioned purified yeast and inoculate it into the yeast liquid medium at a mass ratio of 1:9, and activate it at 25-30°C for 18-24 hours to obtain an activated yeast culture. Add the acclimatization and screening medium at a mass ratio of 1:1000 and mix it to obtain th...

example 1

[0038] Yeast plate culture medium: in parts by mass, take 12 parts of glucose, 8 parts of yeast extract, 5 parts of peptone, 0.02 parts of magnesium sulfate, 15 parts of agar, with a natural pH value, and sterilize at 121°C for 20 minutes.

[0039] Yeast liquid medium: remove the agar in the yeast plate medium, and keep other components unchanged.

[0040] Acclimatization and screening medium: by mass fraction, take 8 parts of glucose, 15 parts of yeast extract, 5 parts of peptone, 0.02 parts of magnesium sulfate, 15 parts of agar, 10 parts of sodium selenite, natural pH, sterilized at 121 °C for 20 minutes .

[0041] Fermentation medium: in parts by mass, take 10 parts of glucose, 18 parts of yeast extract, 1 part of dipotassium hydrogen phosphate, 10 parts of beef extract, 8 parts of peptone, 10 parts of sodium selenite, pH4.8±0.2, 121 Sterilize at ℃ for 20min.

[0042] Raw materials: soil from Bama County, Guangxi.

[0043] A preparation method of selenium-enriched yeast...

Embodiment 2

[0049] Yeast plate culture medium: in parts by mass, take 13 parts of glucose, 9 parts of yeast extract, 6 parts of peptone, 0.03 part of magnesium sulfate, 17 parts of agar, with natural pH value, and sterilize at 121°C for 20 minutes.

[0050] Yeast liquid medium: remove the agar in the yeast plate medium, and keep other components unchanged.

[0051] Acclimatization and screening medium: by mass fraction, take 9 parts of glucose, 16 parts of yeast extract, 6 parts of peptone, 0.03 parts of magnesium sulfate, 17 parts of agar, 11 parts of sodium selenite, natural pH, sterilized at 121°C for 20 minutes .

[0052] Fermentation medium: in parts by mass, take 11 parts of glucose, 19 parts of yeast extract, 1 part of dipotassium hydrogen phosphate, 13 parts of beef extract, 9 parts of peptone, 11 parts of sodium selenite, pH4.8±0.2, 121 Sterilize at ℃ for 20min.

[0053] Raw materials: soil from Bama County, Guangxi.

[0054] A preparation method of selenium-enriched yeast, co...

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PUM

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Abstract

The invention relates to the technical field of fermentation industry, in particular to a preparation method of selenium-rich yeast. A soil sample from Bama County, Guangxi is selected, the content ofselenium in Bama soil and grains is 10 times or more higher than the national average, a selenium-rich yeast strain is obtained through screening and breeding of yeast strains in the soil sample, theselenium ion concentration and the culture condition are optimized, and the selenium content of the yeast is increased. The selenium-rich yeast obtained through separation and screening of the selenium-containing soil is subjected to domestication with sodium selenite with gradient concentrations, yeast colonies with high selenium resistance are screened, then, ultraviolet-induced mutation is performed, the selenium enrichment capability of the yeast is enhanced, and the yeast with high biomass and selenium enrichment capability is obtained.

Description

technical field [0001] The invention relates to the technical field of fermentation industry, in particular to a preparation method of selenium-enriched yeast. Background technique [0002] As we all know, selenium is an essential trace element in humans and animals, and an important component of glutathione peroxygen compounds in organisms. It has many biological functions, the most important of which is to remove the There are too many active oxygen free radicals, so it has the effect of improving the immunity of the organism, anti-aging and anti-cancer. In addition, environmental arsenic poisoning is a worldwide problem. Its hazards are not only harmful to human and animal health in the short term, but also cause long-term malignant changes in the human body—carcinogenesis, mutation and distortion. According to the medical principle that arsenic and selenium can be antidotes to each other, and selenium has the biological functions of inhibiting and anti-cancer, anti-muta...

Claims

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Application Information

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IPC IPC(8): C12N15/01C12N13/00C12N1/36C12N1/16
CPCC12N1/16C12N1/36C12N13/00C12N15/01
Inventor 张德钊李璐尹凯欣
Owner 常州禾吉纺织品有限公司
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