Method used for improving traditional fermentation food quality through co-culturing of saccharomyces cerevisiae and ester producing yeast
A technology of Saccharomyces cerevisiae and Saccharomyces cerevisiae strains, which is applied in the field of Saccharomyces cerevisiae strains, can solve the problems of reducing the application effect and low ethyl acetate content, and achieve the effects of increasing production, high ethanol production, and optimizing culture conditions
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[0039] Example 1 Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) The separation of YF1914
[0040] After crushing and mixing the Daqu evenly, weigh 25 g, add 225 mL of sterile water, shake well and soak for 15 minutes to make a suspension. Under aseptic operating conditions, take 0.1 mL of the suspension and dilute it gradually with sterile water to 10 -3 , 10 -4 , 10 -5 , 10 -6 . Take 0.1 mL of each gradient suspension on the YPD plate, make three parallels for each dilution gradient, and apply sequentially from low concentration to high concentration. Place the medium plate at 30 o Cultivation in a constant temperature incubator for 2 days, and observe the growth of colonies. Pick up a single colony that is spherical, protruding, milky white and opaque on the plate, and inoculate it on the YPD plate with multiple streaks until the morphology of the bacteria is consistent under the microscope. Inoculate a single colony in 50 mL ethanol fermentation medium, 30 o C. Stand ...
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[0041] Example 2 Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) Preservation of YF1914
[0042] Saccharomyces cerevisiae obtained above ( Saccharomyces cerevisiae ) YF1914 is saved by the following methods:
[0043] (1) Slope preservation: Inoculate the purified strains on the YPD slant, place it in an incubator for 48-72 h, o C keep in the refrigerator.
[0044] (2) Preservation in glycerol tube: Take 6 mL of 20% glycerol in the purified strain plate, scrape the colony and mix it with 20% glycerin, divide the mixture into 1.5 mL sterile PE tubes, o Save under C.
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[0045] Example 3 Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) Identification of YF1914
[0046] The above-mentioned Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) The identification of YF1914 includes the following steps:
[0047] Step 1: Morphological characteristics
[0048] In order to identify the aforementioned yeast strains, the following morphological characteristics were observed:
[0049] (1) Colony morphology and cell morphology: The pure culture of the strain obtained in Example 1 was inoculated on WL medium, and its morphological characteristics were observed after 3 days. The result is figure 1 , The colony is milky white, the surface is smooth and moist, the edges are regular, and the center is slightly raised. Observe it after magnifying 1000 times under an optical microscope, the result is as follows figure 2 , The cells are mostly oval or fusiform, budding.
[0050] Step 2: Molecular biology identification
[0051] In order to identify the aforementio...
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