Culture medium for promoting growth of mesenchymal stem cells and preparation method thereof

A mesenchymal stem cell and culture medium technology, which is applied in the field of new medium for promoting the growth of mesenchymal stem cells and its preparation, can solve the problems of slow cell proliferation, high price and cost, and various components of serum substitutes. To clarify the effect of simplicity, low cost, and avoidance of immune rejection

Active Publication Date: 2019-05-17
嘉文丽(福建)化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although serum substitutes avoid the potential disadvantages of serum, they have many components and high cost, and the cultured cells proliferate slowly, which are the main problems that need to be solved urgently in scientific research and medical applications.

Method used

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  • Culture medium for promoting growth of mesenchymal stem cells and preparation method thereof
  • Culture medium for promoting growth of mesenchymal stem cells and preparation method thereof
  • Culture medium for promoting growth of mesenchymal stem cells and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 A kind of culture medium that promotes the growth of mesenchymal stem cells

[0038] The stem cell culture medium is composed of basal medium and additives, and the added additives are calculated at the final concentration, including: Hibiscus hibiscus extract 80ng / mL, seaweed polysaccharide 30ng / mL, astragaloside IV 25ng / mL, human albumin 30μg / mL mL, transferrin 10μg / mL, glutamine 3nM / mL, platelet-derived factor 10ng / mL, epidermal growth factor 10ng / mL, fibroblast growth factor 10ng / mL, human insulin growth factor 10ng / mL, vitamin A 3ng / mL mL.

[0039] The basal medium is DMEM (Dulbecco's modified Eagle medium) or F12 medium.

[0040] The medium preparation method is as follows:

[0041] (1) Accurately weigh the corresponding amount of Hibiscus hibiscus extract, seaweed polysaccharide, astragaloside IV, human albumin, transferrin, glutamine, platelet-derived factor, epidermal growth factor, fibroblast growth factor, human insulin growth factor , Vitamin...

Embodiment 2

[0044] Embodiment 2 A kind of culture medium that promotes the growth of mesenchymal stem cells

[0045] The stem cell medium is composed of basal medium and additives, and the added additives are calculated at the final concentration, including: Hibiscus hibiscus extract 120ng / mL, seaweed polysaccharide 50ng / mL, astragaloside IV 40ng / mL, human albumin 50μg / mL mL, transferrin 20μg / mL, glutamine 5nM / mL, platelet-derived factor 20ng / mL, epidermal growth factor 20ng / mL, fibroblast growth factor 18ng / mL, human insulin growth factor 20ng / mL, vitamin A 5ng / mL mL.

[0046] The basal medium is DMEM (Dulbecco's modified Eagle medium) or F12 medium.

[0047] The medium preparation method is as follows:

[0048] (1) Accurately weigh the corresponding amount of Hibiscus hibiscus extract, seaweed polysaccharide, astragaloside IV, human albumin, transferrin, glutamine, platelet-derived factor, epidermal growth factor, fibroblast growth factor, human insulin growth factor , Vitamin A, hib...

Embodiment 3

[0051] Embodiment 3 A kind of culture medium that promotes the growth of mesenchymal stem cells

[0052] The stem cell medium is composed of basal medium and additives, and the added additives are calculated at the final concentration, including: Hibiscus hibiscus extract 110ng / mL, seaweed polysaccharide 40ng / mL, astragaloside IV 30ng / mL, human albumin 45μg / mL mL, transferrin 15μg / mL, glutamine 4nM / mL, platelet-derived factor 15ng / mL, epidermal growth factor 15ng / mL, fibroblast growth factor 15ng / mL, human insulin growth factor 15ng / mL, vitamin A 4ng / mL mL.

[0053] The basal medium is DMEM (Dulbecco's modified Eagle medium) or F12 medium.

[0054] The medium preparation method is as follows:

[0055] (1) Accurately weigh the corresponding amount of Hibiscus hibiscus extract, seaweed polysaccharide, astragaloside IV, human albumin, transferrin, glutamine, platelet-derived factor, epidermal growth factor, fibroblast growth factor, human insulin growth factor , Vitamin A, hib...

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Abstract

The invention discloses a culture medium capable of promoting the division growth of mesenchymal stem cells, which comprises a serum-free basic culture medium and an additive added on the basis of theserum-free basic culture medium, wherein the additive comprises a hibiscus mutabilis extract, seaweed polysaccharide, astragaloside IV, human serum albumin, transferrin, glutamine, platelet-derived factor, epidermal growth factor, fibroblast growth factor, human insulin growth factor and vitamin A; the hibiscus mutabilis extract has antioxidant and cell activating activity, and can effectively promote the growth of cell metabolic by compounding seaweed polysaccharide and astragaloside IV; human serum albumin, transferrin, glutamine and vitamin A provide essential nutrient substances for the growth of stem cells; platelet-derived factor, epidermal growth factor, fibroblast growth factor, human insulin growth factor and other cytokines together promote the rapid growth and proliferation ofstem cells; the culture medium not only can improve the growth activity of mesenchymal stem cells, shorten the culture time, promote the expression of cell growth factors, but also can maintain the stem cell activity of the differentiation potential of mesenchymal stem cells; stem cells are not differentiated in daily culture, thereby providing convenience for scientific research.

Description

technical field [0001] The invention belongs to the technical field of stem cell culture medium, and in particular relates to a novel culture medium for promoting the growth of mesenchymal stem cells and a preparation method thereof. Background technique [0002] Stem cells are a kind of primitive undifferentiated cells with multi-differentiation potential and self-renewal ability. According to the developmental stage of stem cells, they can be divided into embryonic stem cells and adult stem cells. Embryonic stem cells are also called "seed cells" and can differentiate into various types of tissue cells; adult stem cells are undifferentiated cells that exist in differentiated tissues. Such as neural stem cells in the nervous system, blood stem cells in the blood circulation system, fat stem cells in adipose tissue, etc. Stem cells can be divided into totipotent stem cells (such as embryonic stem cells), pluripotent stem cells (such as mesenchymal stem cells) and unipotent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
Inventor 饶焕文林建平萧自智潘发伍叶美娜高媛媛
Owner 嘉文丽(福建)化妆品有限公司
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