Double-chamber three-dimensional pouring bioreactor system

Abstract

The invention discloses a double-chamber three-dimensional pouring bioreactor system which comprises a bioreactor, wherein the bioreactor is internally provided with a three-dimensional bracket and is connected with a pouring device. The bioreactor comprises a first chamber filled with common nutritional nutrient liquor and a second chamber filled with inductive culture factors. A dialysis membrane assembly through which components having molecular weight being less than the molecular weight of inductive culture factors in the inductive culture factors can pass is arranged between the first chamber and the second chamber. The three-dimensional bracket is located in the second chamber. According to the double-chamber structural design of the bioreactor disclosed by the invention, the common nutritional nutrient liquor and the inductive culture factors in the two chambers are independently exchanged, and the inductive culture factors can be preserved while the common nutritional nutrient liquor is changed, so that the use level of the inductive culture factor is greatly reduced, the effective utilization ratio of the inductive culture factors is improved, and the cost is lowered.

Description

technical field [0001] The invention belongs to the field of bioengineering equipment, in particular to a double-cavity three-dimensional perfusion bioreactor system in which cells grow and differentiate in a three-dimensional support. Background technique [0002] At present, in the field of life science research and medical field, there are many cell culture methods, which can basically be classified into two-dimensional and three-dimensional culture methods according to the dimensions of culture. In two-dimensional culture methods, such as glass or plastic culture bottles and culture dishes, it is necessary to provide a plane for cell attachment and growth, and to provide a better growth environment for cells by modifying the type of plane material and the contact surface, and also through an inverted microscope And ordinary microscopes can easily observe the growth status of cells. However, normal cells grow in a three-dimensional environment in both human and animal b...

AI Technical Summary

Problems solved by technology

In order to promote the growth and differentiation of cells, the culture medium in the above-mentioned bioreactor generally includes common nutrient culture medium and induced culture factors, and common nutrient culture medium generally includes basic nutrients such as sugar, amino acids, trace elements, etc. to maintain cell growth and proliferation , also includes buffer to adjust the appropriate physiological pH value, while inducible culture factors play a role as a key component of cell growth and differentiation, but are extremely expensive because of their difficulty in obtaining and low yield

Since the above-mentioned bioreactor has only one reaction vessel, the growth culture medium and the induction culture factor must be mixed in the same reaction vessel, and the amount of the induction culture factor required for one cultivation is very large; the common nutrient culture medium is relatively cheap, for To meet the needs of cell growth, the culture medium needs to be replaced frequently. Every time the ordinary nutrient culture medium is replaced, the induced culture factors will also be lost. The cost of such culture is relatively high.

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