Collagen/chondroitin sulfate composite artificial cornea and preparation method thereof

A technology of chondroitin sulfate and composite membrane, which is applied in medical science, prosthesis, etc.

Inactive Publication Date: 2017-07-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Most of the researches on the artificial cornea in the domestic and international literature at present focus on the main raw material being a synthetic polymer, and the collagen and chondroitin sulfate, which are the main components of the corneal tissue involved in the present invention, have not been reported as raw materials.

Method used

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  • Collagen/chondroitin sulfate composite artificial cornea and preparation method thereof
  • Collagen/chondroitin sulfate composite artificial cornea and preparation method thereof
  • Collagen/chondroitin sulfate composite artificial cornea and preparation method thereof

Examples

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Embodiment 1

[0025] Weigh 10 mg of collagen freeze-dried raw materials, dissolve them in 10 mg of ionic liquid, stir and dissolve at 55°C for 2-4 hours, centrifuge the completely dissolved collagen solution at 4000 rpm for 10 min to de-bubble, and pour it into a mold by casting to spread it into a film . The mold is immersed in a 65-95% ethanol coagulation bath for 2-5 hours for regeneration and molding, and then immersed and washed with an 80% ethanol aqueous solution for 3 times to remove the ionic liquid in the gel film to obtain a gel sheet with a thickness of 0.2 mm. Dehydrate in a constant temperature oven at ℃ to form a dry collagen film Col-1.

Embodiment 2

[0027] Weigh 100 mg of collagen freeze-dried raw materials, dissolve them in 100 mg of ionic liquid, stir and dissolve at 55°C for 2 days, centrifuge the completely dissolved collagen solution at 4000 rpm for 10 min to de-bubble, and pour it into a mold using a casting method to spread it into a film. Immerse the mold in a 65-95% ethanol coagulation bath for 2-5 hours to regenerate the molding. Use an 80% ethanol aqueous solution to soak and wash three times to remove the ionic liquid from the gel film to obtain a gel sheet with a thickness of 02mm. Dehydrate in the incubator to form a dry collagen film Col-2.

Embodiment 3

[0029] Weigh 300 mg of collagen freeze-dried raw material, dissolve it in 300 mg of ionic liquid, stir and dissolve at 55°C for 3 days, centrifuge the completely dissolved collagen solution at 4000 rpm for 10 min to de-bubble, and pour it into a mold by casting to spread it into a film. The mold is immersed in a 65-95% ethanol coagulation bath for 2-5 hours for regeneration and molding, and then immersed and washed with an 80% ethanol aqueous solution for 3 times to remove the ionic liquid in the gel film to obtain a gel sheet with a thickness of 0.2 mm. Dehydrate in a constant temperature oven at ℃ to form a dry collagen film Col-3.

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Abstract

The invention discloses a preparation method of a collagen / chondroitin sulfate composite artificial cornea. The preparation method comprises the following steps: dissolving collagen into a water solution of imidazole ionic liquid to obtain a collagen solution (1-30%); then dissolving chondroitin sulfate into a water solution to obtain a chondroitin sulfate water solution (1-20 mg / mL); evenly mixing two solutions according to a certain ratio, adding a natural crosslinking agent (EDC / NHS) with a good biocompatibility to prepare collagen / chondroitin sulfate in-situ crosslinked composite hydrogel; soaking the composite hydrogel in a coagulating bath to carry out moulding and regeneration, washing the hydrogel by water to remove the solvent, naturally drying to obtain a collagen / chondroitin sulfate composite membrane; dissolving a corneal growth factor FGF-10 into a phosphoric acid buffer solution to obtain a FGF-10 water solution (1-100 [mu]g / mL) ; and soaking the composite membrane into the FGF-10 water solution to prepare a collagen based membrane Col / Cs / FGF with a sustained release effect. The provided novel artificial cornea has the advantages of high strength, sustained release, and biocompatibility, and has an important application prospect.

Description

Technical field [0001] The invention relates to a preparation method of a collagen / chondroitin sulfate composite artificial cornea, which belongs to the field of biological materials. Background technique [0002] Globally, the number of patients with corneal disease is as high as 300 million, and this data is increasing year by year. At the same time, due to the severe shortage of corneal donors and other problems such as uncontrollable safety, the treatment of corneal diseases is difficult. The new idea of ​​using tissue engineering technology to prepare artificial cornea in vitro has attracted the attention of the majority of scientific researchers. [0003] At present, the research of artificial cornea mainly focuses on the materials of optical lens column and stent. Optical lens column materials require good light transmittance. Polymethyl methacrylate (PMMA) is generally used, but there are problems such as high hardness, high pressure steam sterilization, and poor surface ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/52A61L27/54A61L27/56C08J3/24C08J5/18
CPCA61L27/48A61L27/52A61L27/54A61L27/56A61L2300/414C08J3/246C08J5/18C08J2389/00C08J2405/08C08L5/08C08L89/00
Inventor 袁琬娟许淑琴陈敬华胡斌李响单恒悦王颖
Owner JIANGNAN UNIV
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