Biomedical mouse tail collagen extraction method
A rat tail collagen, biomedical technology, applied in peptide preparation methods, animal/human proteins, chemical instruments and methods, etc., can solve the problems of no extraction and purification process of biomedical rat tail collagen, and improve application safety , improve biocompatibility, and ensure the effect of biological functionality
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[0032] Example 1
[0033] Extraction of Biomedical Rat Tail Collagen
[0034] A method for extracting biomedical rat tail collagen, the steps are as follows:
[0035] (1) Take 1 kg of fresh rat tail, soak it in 1:1000 Xinjiere solution for 10 to 15 min, and rinse it with normal saline for more than 5 times after taking it out; separate the rat tail tendon and remove the residual tissue fibers of the rat tail tendon. NH 4 Cl - Soak in physiological saline solution for 24h, keep stirring at low speed for 24h at 4°C, and change the solution twice to remove residual fat and polysaccharide. Rinse with deionized water 5 times, pre-freeze at -20°C for 4 hours, and freeze-dried in vacuum for use; the rat tails are from adult nutria, rat tails of various strains or mouse rat tails of various strains.
[0036] (2) Freeze-dried rat tail tendon is lyophilized and crushed to 200-400 mesh;
[0037] (3) swelling the rat tail tendon in 0.06mol / L acetic acid solution for 2 hours in an ice...
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[0043] Example 2
[0044] A method for extracting biomedical rat tail collagen, the steps are as follows:
[0045] (1) Take 1kg of fresh rat tail, soak it in 1:1000 Xinjieer solution for 10 to 15 minutes, and rinse it with normal saline for more than 5 times after taking it out; separate the rat tail tendon and remove the residual tissue fibers of the rat tail tendon. NH 4 Cl - Soak in physiological saline solution for 24h, keep stirring at low speed for 24h at 4°C, and change the solution twice to remove residual fat and polysaccharide. Rinse with deionized water for 5 times, pre-freeze at -40°C for 2 hours, and freeze-dry in vacuum for use;
[0046] (2), freeze-dried rat tail is pulverized to 200 mesh through low temperature freeze-drying;
[0047](3) Swell the pulverized rat tail tendon in an acetic acid solution in an ice-water bath, the solid-to-liquid ratio of rat tail tendon and acetic acid is 1 g: 100 mL, the concentration of the acetic acid solution is 0.04 mol / L,...
Example Embodiment
[0053] Example 3
[0054] A method for extracting biomedical rat tail collagen, the steps are as follows:
[0055] (1) Take 1 kg of fresh rat tail, soak it in 1:1000 Xinjieer solution for 10 to 15 min, and rinse it with normal saline for more than 5 times after taking it out; separate the rat tail tendon and remove the residual tissue fibers of the rat tail tendon. NH 4 Cl - Soak in physiological saline solution for 24h, keep stirring at low speed for 24h at 4°C, and change the solution twice to remove residual fat and polysaccharide. Rinse with deionized water for 5 times, pre-freeze at -40°C for 2 hours, and freeze-dry in vacuum for use;
[0056] (2), freeze-dried rat tail is pulverized to 400 mesh by low temperature freeze-drying;
[0057] (3) Swell the pulverized rat tail tendon in an acetic acid solution in an ice-water bath, the solid-to-liquid ratio of rat tail tendon and acetic acid is 1 g: 120 mL, the concentration of the acetic acid solution is 0.1 mol / L, and the...
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