Ultra-low temperature preserving method for nutrient breeding flower and short-tube lycoris

A technology of ultra-low temperature preservation and vegetative reproduction, which is applied in the field of ultra-low temperature preservation of vegetatively propagated flowers of Lycoris chinensis, can solve the problem that the ultra-low temperature preservation technology of Lycoris is not yet established, and achieves the effect that the ultra-low temperature preservation method is simple and easy to implement and restores a good growth condition.

Inactive Publication Date: 2007-03-28
SHANGHAI AGROBIOLOGICAL GENE CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the cryopreservation technology for Lycoris has not been established in the world

Method used

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  • Ultra-low temperature preserving method for nutrient breeding flower and short-tube lycoris
  • Ultra-low temperature preserving method for nutrient breeding flower and short-tube lycoris
  • Ultra-low temperature preserving method for nutrient breeding flower and short-tube lycoris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 3-4 year old bulbs of Lycoris radiata Herb, disinfect with 70% alcohol for 1 min, 0.1% mercuric chloride for 15 min, rinse with sterile water for several times and remove 3-4 layers of scales and part of the stem disk. Bulb cuts (about 1cm in size) were inoculated with 3mg·L -1 BA and 0.5 mg·L -1 Induction of bulblets on MS medium with NAA. The culture temperature is 25±2℃, and the light is 12h·d -1, light intensity 36μmol m -2 the s -1 .

Embodiment 2

[0024] Example 2 Lycoris radiata Herb (Lycoris radiata Herb) pre-cultivation

[0025] Get the bulblet of embodiment 1 gained, cut out the shoot tip that has root primordia and bud primordia simultaneously of 2.5mm size, inoculate to contain 0.4mol L -1 In sucrose MS medium, culture in dark at 25°C for 5 days.

Embodiment 3

[0027] Put the pre-cultured shoot tips in the pretreatment solution (2mol L -1 glycerol and 0.4mol L -1 Sucrose MS liquid medium) was treated at room temperature for 20min, then transferred to vitrification agent (3.26mol·L -1 Glycerin+2.42mol·L -1 Ethylene glycol+1.9mol·L -1 DMSO+0.4mol L -1 sucrose) in ice bath for 80min, and then transfer the shoot tips to 2mL cryovials filled with pre-cooled fresh vitrification agent (10 shoot tips·tube -1 ), quickly put into liquid nitrogen for storage.

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Abstract

The present invention relates to an ultra-low temperature storage method of nutrient propagative flower plant lycoris radiate. Said method includes the following steps: bulb induction, pre-culture of shoot tip with root rudiment and bud rudiment, placing the above-mentioned pre-cultured shoot tip in pretreatment solution to make pretreatment at room temperature for 10-30 min, then placing it into vitrified protective agent to make ice bath treatment for 70-90 min, then transplanting said shoot tip into a freeze tube in which the precooled fresh vitrified protective agent is held and placing said freeze tube into liquid nitrogen so as to make storage.

Description

technical field [0001] The invention relates to a method for preserving lycoris, a vegetatively propagated flower, in particular to an ultra-low temperature preservation method for lycoris, a vegetatively propagated flower. Background technique [0002] Lycoris plants are perennial flower bulbs, distributed from southwest to southeast of my country, and are one of the precious flowers in China. It may be caused by human transmission, or natural distribution and evolution. At present, its distribution has been reported from the southeast of China to the coastal islands, the Korean Peninsula, the Ryukyu Islands, and Japan's Kyushu, Shikoku and Main Island, but more than 70% Lycoris resources are distributed in our country. When Lycoris blooms, there are no leaves to accompany it. The flower stems emerge from the bare ground and bloom gorgeous and huge flowers. They are unique and dazzling. They are also called "magic flower", "Chinese tulip" and "surprise lily" in the west. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00
Inventor 刘灶长林田李天菲郑海柔罗利军
Owner SHANGHAI AGROBIOLOGICAL GENE CENT
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