The invention discloses a water phase preparation method of nucleating doped nano-crystal of ZnS: Mn and ZnS: Mn or ZnS, deionized water is regarded as solvents, zinc salt is regarded as the source of zinc ion, manganese salt is regarded as the source of manganese ion, sodium sulfide is regarded as the source of sulfur ion, mercapto carboxylic acid is regarded as stabilizer, water bath or oil bath is used for controlling the reaction temperature, the whole reaction process is carried on in the air, the method has the advantages of the low cost, the simple operation, the whole process being carried on in the air, innocuity, and no pollution, the nano-crystal of ZnS: Mn and ZnS: Mn or ZnS has the high strength, the good light stability, and the good water solubility, the method of the invention can realize the mass production of the nano-crystal of ZnS: Mn and ZnS: Mn or ZnS.