New method for cryopreserving hematopoietic stem cells

Abstract

The invention discloses a new method for cryopreserving hematopoietic stem cells. The new method comprises the following steps: step 1, taking a hematopoietic stem cell protection agent, a hematopoietic stem cell suspension solution or bone marrow fluid at the volume ratio of (1-2) to 6; step 2, uniformly mixing the hematopoietic stem cell suspension solution or the bone marrow fluid with the hematopoietic stem cell protection agent; emptying air and sealing, and storing the mixed solution in a refrigerator in a sealed manner at -80 DEG C for 12 hours; and step 3, taking out the mixed solution of the hematopoietic stem cell suspension solution or the bone marrow fluid and the hematopoietic stem cell protection agent; and then adding the hematopoietic stem cell protection agent, wherein the volume ratio of the hematopoietic stem cell protection agent to the mixed solution is 1 to 3; and emptying air and sealing again, and storing the mixed solution in the refrigerator in a sealed manner at -80 DEG C. The cryopreserving method provided by the invention is simple and convenient, is easy to operate and has a relatively good cryopreserving effect.

Description

technical field [0001] The invention relates to a cryopreservation method for hematopoietic stem cells. Background technique [0002] Hematopoietic stem cells are adult stem cells in the blood system. They are a heterogeneous group with the ability of long-term self-renewal and the potential to differentiate into various mature blood cells. It is a type of adult stem cell with the longest and most in-depth research history, and it has important guiding significance for the study of various stem cells, including tumor stem cells. Mature cells in the blood system have a very short lifespan. Therefore, in a person's life, hematopoietic stem cells need to replenish various mature cell components of the blood system in a timely manner according to the physiological needs of the body. At the same time, under stress conditions such as injury and inflammation, hematopoietic stem cells also play a role in regulating and maintaining the physiological balance of various cell component...

AI Technical Summary

Problems solved by technology

At present, the protective agents used for low-temperature storage of stem cells at -80°C include dimethyl sulfoxide (hereinafter referred to as DMSO), CP-1, hydroxyethyl starch, RPMI1640 medium, human serum albumin, etc. The methods of use are quite different, and the preservation effect and storage time of stem cells are also different. The survival rate of most preserved cells is about 80%. It is difficult to guarantee the safety of stem cells. Some cryopreservation methods also have the disadvantage of complicated operation.

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