A high-quality cattle and sheep oocyte incubator based on embryo in vitro culture technology

Abstract

The invention discloses a high-quality cattle and sheep oocyte incubator based on embryo in vitro culture technology. The body is provided with a plurality of culture chambers, the culture plate is set in the culture chamber, the bottom of the box body is provided with a cavity matching the receiving plate, and the side wall of the box body is provided with a first chute, the receiving plate is provided with a slider matching the first chute, the inner wall of the cavity is provided with a second chute matching the first chute, the receiving plate A first through cavity is provided on the top, a first baffle is provided in the culture cavity, a second baffle is provided in the first through cavity, and a through hole is provided on the side wall of the first through cavity; Multiple culture boxes are detachably connected to the culture plate; the number of culture boxes can be selected according to the actual number of cells to be cultured to prevent waste of culture boxes and reduce experimental costs.

Description

technical field [0001] The invention belongs to the technical field of cell culture devices, in particular to a high-quality cattle and sheep oocyte incubator based on embryo in vitro culture technology. Background technique [0002] With the advancement of embryo engineering technology, embryo biotechnology has been widely used in more and more fields, and has promoted the development of many disciplines such as medicine, medicine, animal husbandry, genetic engineering, and animal protection. In vitro culture is the core of embryo biotechnology. Both oocytes and embryos need to be cultured in vitro. At present, culture dishes and culture plates are mainly used. There are multiple culture wells on the culture plate, generally 6, 12, 24, 48, and 96 wells. Only one culture well or a few culture wells are used in each batch of experiments because of concerns that the probability of cell contamination will increase. The remaining culture wells on the culture plate are rarely re...

AI Technical Summary

Problems solved by technology

There are multiple culture wells on the culture plate, generally 6, 12, 24, 48, and 96 wells. Only one culture well or a few culture wells are used in each batch of experiments because of concerns that the probability of cell contamination will increase. The remaining culture wells on the culture plate are rarely reused, resulting in increased experimental costs

Due to the large volume of the existing culture dish, it is not suitable for in vitro culture of few cells, and the whole culture dish needs to be covered with paraffin oil, which not only wastes space but also wastes paraffin oil; if multiple groups of cells are cultivated in one culture dish, it can Avoid waste, but the distance between the culture droplets in the petri dish is relatively close, and the shaking of the petri dish will cause the mixing of different treatment droplets, resulting in the failure of the experiment

[0003] In order to ensure the survival rate of cells, the petri dish usually needs to be placed in an incubator for cultivation. The environment in the chamber is suitable for cell growth and is different from the external environment. During the culture process, the cells need to open the incubator frequently to observe the cells , the outside air directly enters the incubator when the incubator is opened, which affects the gas balance in the incubator, thereby affecting the survival rate of oocytes

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