Separation and purification method for Russula depalleus (Pers.) Fr. spawns
A technology for separation and purification of mycelium, applied in the field of wild mushroom mycelium separation and purification, can solve the problems of low success rate of mycelium separation, single, unfavorable mycelium growth, etc., and achieve the effect of easy operation, simple process and convenient operation
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Embodiment 1
[0036] A method for separating and purifying purple-black russula mycelia, specifically comprising the following steps:
[0037] (1) Disinfection of wild purple and black russula strains
[0038]Disinfect the tissues or spores of wild Russula russula; sterilize the tissues of wild Russula with 45% alcohol solution ultrasonically, the ultrasonic temperature is 21°C, the power is 32W, and the action time is 6min; then under sterile conditions, Cut longitudinally and tear apart the stipe or the middle part of the cap with a surgical blade that has been burnt by flame, and pick a tissue block with a length of 0.4 cm and a width of 0.2 cm at the junction of the cap and the stipe; carry out ultraviolet disinfection on the spores : Ultraviolet rays with a wavelength of 280nm are used to sterilize for 12s at a temperature of 21°C and an ambient relative humidity of 60%; wild purple and black red mushroom tissues are selected from the joints of the stipe and the cap. It is easy to dis...
Embodiment 2
[0053] A method for separating and purifying purple-black russula mycelia, specifically comprising the following steps:
[0054] (1) Disinfection of wild purple and black russula strains
[0055] Disinfect the tissues or spores of wild Russula russula; sterilize the tissues of wild Russula with 50% alcohol solution ultrasonically, the ultrasonic temperature is 22°C, the power is 35W, and the action time is 7min; then under sterile conditions, Use a flame-burned scalpel to cut longitudinally and tear apart the middle part of the stipe or cap, and pick a tissue block with a length of 0.5 cm and a width of 0.3 cm at the junction of the cap and the stipe; carry out ultraviolet disinfection on the spores : Ultraviolet rays with a wavelength of 300nm are used to sterilize for 15s at a temperature of 22°C and a relative humidity of 65%;
[0056] Wipe the surface of the separated tissue block with 70% alcohol cotton ball, and wrap the tissue with alcohol cotton for 2 minutes; make th...
Embodiment 3
[0070] The optimal conditions for the mycelium growth of purple-black russula mushroom were determined by orthogonal experiment method, which was determined by measuring the growth rate and growth amount of the mycelia;
[0071] Wherein, the method for measuring the growth rate of mycelia is: place the culture medium plate in step 6 on the microscope stage, check the eyepiece micrometer of the microscope used at the same time, and calculate the length of each grid; select the top of a single mycelium at the edge of the colony Focus under a low magnification lens; then put the eyepiece micrometer parallel to the hyphae, and select the part where the hyphae begins to appear side branches coincides with a line on the eyepiece micrometer, this point is the "reference point"; Measure the length of mycelium growth once in a certain period of time. After several observations, the growth rate of mycelium can be calculated according to the time and the length of the top extension;
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