Liver cancer cell system from liver cancer portal vein tumor thrombus of human and system establishing method thereof

Abstract

The invention relates to the field of microorganism animal cells, in particular to a liver cancer cell system from liver cancer portal vein tumor thrombus of human and a system establishing method thereof. The invention adopts the following technical scheme that the liver cancer cell system from liver cancer portal vein tumor thrombus of human is preserved in the China Center for Type Culture Collection (CCTCC) and has a preservation number of CCTCCNO: C200814. The system establishing method of the liver cancer cell system from liver cancer portal vein tumor thrombus of human comprises the following steps: (1) acquiring cancer embolus cells and carrying out a primary culture on the embolus cells; (2) carrying out a clone expansion culture on a tumor cell island; and (3) carrying out a continuous culture on tumor cells. The invention has the advantages that CSQT-1 cells are obtained from the portal vein tumor embolus and have incomparable advantages on the forming mechanism and the tendency growing mechanism of the portal vein tumor embolus than other existing liver cancer systems. The cell system has wide prospect of application, is a sub-hypotetraploid displayed by the karyotype analysis and accounts for 58 percent of the total.

Description

technical field

[0001] The invention relates to the field of microbial animal cells, in particular to a liver cancer cell line derived from portal vein tumor thrombus of human liver cancer and a method for establishing the line. Background technique

[0002] Primary liver cancer (HCC) is one of the most common malignant tumors, and the median survival time of untreated patients is less than one year. Surgical resection is currently the most effective treatment for liver cancer. But even if the tumor is removed, most patients still die of postoperative tumor recurrence and metastasis. The main feature of liver cancer metastasis is to grow into the portal vein to form portal vein tumor thrombus. Shanghai Oriental Hepatobiliary Surgery Hospital performed liver tumor resection on 5,524 patients with liver cancer, and the proportion of portal vein tumor thrombi found in the resected specimens reached 67.1% (Chinese Journal of Surgery, 2001, 39: 25-28); The study of growth char...

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