Bifidobacterium longum microcapsule and preparation method thereof

A technology of bifidobacteria longum and microcapsules, applied in bifidobacteria, food preparation, bacteria used in food preparation, etc., can solve problems such as the decrease in the number of viable bacteria, and achieve longer shelf life, less survival, and tolerance. low effect

Inactive Publication Date: 2014-07-23
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the bactericidal effect of gastric acid, the number of viable bacteria will drop significantly during this process. Switzerland and the I

Method used

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  • Bifidobacterium longum microcapsule and preparation method thereof
  • Bifidobacterium longum microcapsule and preparation method thereof
  • Bifidobacterium longum microcapsule and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Bifidobacterium longum BBMN68 is used as the core material, and sodium alginate and whey protein are used as the wall material

[0044] (1) Preparation of bacterial strains: In the ultra-clean workbench, inoculate 0.1ml of the frozen Bifidobacterium longum BBMN68 bacterial solution into 10ml of MRS liquid medium, and use an anaerobic tube in a constant temperature and humidity box at 36.5- Cultivate at a temperature of 37.5°C for 12 hours, repeat passage for 2-3 times, and obtain activated Bifidobacterium longum.

[0045] The activated Bifidobacterium longum was inoculated into 250ml of MRS liquid medium at a volume ratio of 0.1%, placed in a constant temperature and humidity chamber, cultivated at a temperature of 36.5-37.5°C for 15h, and centrifuged at 4500rpm for 15min to collect the bacteria. Then the thallus was washed with 0.9% physiological saline, centrifuged at 4500 rpm for 15 min, and washed twice to obtain muddy wet thallus of Bifidobacterium longum...

Embodiment 2

[0052] Example 2 Bifidobacterium longum BBMN68 is used as the core material, and sodium alginate, whey protein and glycerin are used as the wall material

[0053] The polysaccharide solution is prepared by dissolving the polysaccharide in 50wt% glycerin aqueous solution. The mass ratio of glycerin, sodium alginate and whey protein in the final product is 12.5:1:1. Other specific preparation methods are the same as in the above-mentioned Example 1.

Embodiment 3

[0055] The specific preparation method is the same as in Example 2, but sodium alginate is dissolved in 50wt% glycerol aqueous solution, wherein the mass ratio of glycerol to sodium alginate is 25:1, and it is mixed with 8wt% whey protein, and the final product is The mass ratio of glycerin, whey protein and chitosan is 25:5:1.

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Abstract

The invention discloses a bifidobacterium longum microcapsule and a preparation method thereof. The core material is bifidobacterium longum BBMN68; the wall material comprises metaprotein and polysaccharide; the core material is mixed with the sterilized wall material; the freeze-drying bifidobacterium longum microcapsule is prepared by orifice-coagulating bath and vacuum freeze drying; the bifidobacterium amount of the microcapsule is 108 to 1011 CFU/g. After the microcapsule is stored at room temperature for 6 months, the amount of the bifidobacterium is still more than 108 CFU/g; major viable count in intestine can be realized after the freeze-drying microcapsule particles get through the stomach, and most of the bifidobacteriums are released in the intestine. The bifidobacterium microcapsule is processed by freeze drying so as to provide low-temperature, vacuum and dry environment for bifidobacterium BBMN68; thus, the microcapsule overcomes the shortcomings that bifidobacterium does not resist acid and oxidation, and is not easy to survive, and the like; moreover, the microcapsule has the advantages of long expiration date, good storage stability and enteric solubility.

Description

technical field [0001] The invention relates to a microcapsule product, in particular to a Bifidobacterium longum BBMN68 microcapsule and a preparation method thereof. Background technique [0002] Bifidobacterium belongs to the genus Bifidobacterium of Actinomycetes. Currently, the strains of human Bifidobacterium used in production include Bifidobacterium bifidum (B. bifidum), Bifidobacterium adolescentis (B. adolescentis), Bifidobacterium infantis Bacillus (B. infantis), Bifidobacterium longum (B. longum) and Bifidobacterium breve (B. breve). Bifidobacteria have no spores, the individual size is 0.5-1.3 μm × 1.5-8 μm, and the typical shape is rod-shaped with forked ends. Its colonies are cream to white in color, convex in shape, soft and elastic in texture, smooth in surface and complete in margin. Bifidobacterium is obligate anaerobic, the optimum growth temperature is 37-41°C, the minimum growth temperature is 25-28°C, the maximum growth temperature is 43-45°C, the op...

Claims

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Application Information

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IPC IPC(8): A23L1/29A23P1/04A23L33/00A23P10/30
CPCA23L33/135A23P10/30A23C9/1234A23V2002/00A23V2400/533A23V2250/54252A23V2250/5026A23V2250/6406A23V2250/511A23V2250/5022
Inventor 冷小京刘云翟晓娜杨丽芳杜秉健刘飞张春月唐晓双马静
Owner CHINA AGRI UNIV
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