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Method for preparing penicillium enol D2 derived from Penicillium citrinum and application of penicillium enol D2

A technology of penicillol and Penicillium citrinum, applied in the field of biopharmaceuticals, to achieve significant anti-tumor activity

Inactive Publication Date: 2015-05-06
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that the alkaloid compound shown has anti-tumor activity, but there is no report on the anti-proliferation activity of this compound on A549, so there is no related drug on the market

Method used

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  • Method for preparing penicillium enol D2 derived from Penicillium citrinum and application of penicillium enol D2
  • Method for preparing penicillium enol D2 derived from Penicillium citrinum and application of penicillium enol D2
  • Method for preparing penicillium enol D2 derived from Penicillium citrinum and application of penicillium enol D2

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Experimental program
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Effect test

Embodiment 1

[0018] Fermentative production and separation and purification of the compound of embodiment 1

[0019] 1 Fermentation production

[0020] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, take Penicillium citrinum ( Penicillium citrinum ) IBPT-5 (preserved in China Center for Type Culture Collection on December 25, 2013, address: Wuhan University, Wuhan, deposit number: CCTCC NO: M 2013713) appropriate amount, inoculated on PDA solid slant medium at 28 Cultivate in an incubator for 4 days.

[0021] Get the Penicillium citrinum ( Penicillium citrinum ) appropriate amount of IBPT-5, inoculated into 400mL culture medium [Culture medium composition (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5, MgSO 4 0.3, NaCl 30.0 constant volume] in a 1000mL Erlenmeyer flask, after 30 days of static culture at 28°C, the mycelia and fermentation broth were obtain...

Embodiment 2

[0030] Example 2 Test of anti-tumor activity in vitro

[0031] 1 Experimental samples and experimental methods

[0032] Preparation of the tested sample solution The test sample is the pure product of the compound separated and refined in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0033] Cell lines and cell subculture Tumor cell lines were used, and tumor cells were subcultured in DMEM medium containing 10% FBS at 37°C in an incubator filled with 5% carbon dioxide.

[0034] Cell Proliferation Inhibitory Activity Test Method

[0035] Tetrazolium salt (MTT) method Take tumor cells in the logarithmic growth phase and adjust the cell density to 2×10 per ml 5 Cells were inoculated into 96-well cell culture plates at 200 microliters per well, and filled with 5% CO at 37°C 2 for 4 hours in an incubator. Add 2 microliters of sample solution...

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Abstract

The invention relates to a method for preparing an alkaloidal compound penicillium enol D2 derived from Penicillium citrinum and an application of penicillium enol D2. The compound has an effect of inhibiting proliferation of tumor cells and has antitumor activity. The structural formula is as shown in the specification. The compound is prepared by the steps: performing fermentation cultivation on Penicillium citrinum IBPT-5, and separating and purifying the compound from the fermented product. The experiment proves that the compound has high antitumor activity on the human lung carcinoma cell line A549. The compound can serve as a medicine for inhibiting cell proliferation or an antitumor drug to be used for antitumor research.

Description

technical field [0001] The present invention relates to a kind of alkaloid compound penicillol D derived from Penicillium citrinum 2 The preparation method and application thereof belong to the field of biopharmaceuticals. Background technique [0002] Alkaloids are a type of nitrogen-containing organic compounds produced by biological secondary metabolism. There are many types of alkaloids in nature, most of which come from plants, so they are also called plant alkaloids. Alkaloids have important physiological effects on humans and animals, including anti-asthma and antitussive, hypoglycemic, hypolipidemic, antibacterial, antitumor, analgesic, etc., among which antibacterial and antitumor activities are the most prominent. Natural structural alkaloids are an important source for discovering lead compounds in the research of innovative drugs, and there are nearly a hundred kinds of alkaloid drugs currently used in clinical practice. Studies have found that some marine fung...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D207/02C12P17/10A61P35/00C12R1/80
CPCC07D207/02C12P17/10
Inventor 陈立周彤赵杨杨张其清
Owner FUZHOU UNIV
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