Human lung adenocarcinoma cell line and application thereof

A technology for human lung adenocarcinoma and cell lines, applied to human lung adenocarcinoma cell lines and its application fields, can solve the problems of large differences in biological characteristics and low biodiversity of lung adenocarcinoma, and achieve good in vitro culture and expansion Sexuality, strong tumorigenicity, and stable properties

Active Publication Date: 2014-12-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a new human lung adenocarcinoma cell line in view of the existing human lung aden

Method used

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  • Human lung adenocarcinoma cell line and application thereof
  • Human lung adenocarcinoma cell line and application thereof
  • Human lung adenocarcinoma cell line and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Preparation of Cells

[0030] Nude mice: 5 nude mice, male, weighing 16.0±1.0 g, aged 4 weeks, were kept in the SPF animal room. Nude mice were uniformly bred by Zhejiang Experimental Animal Center.

[0031] Fresh resection specimens of clinical lung adenocarcinoma (female, 55 years old, primary lung adenocarcinoma, pathological diagnosis: adenocarcinoma) were obtained from the Second Affiliated Hospital of Zhejiang University School of Medicine, and immediately immersed in pre-cooled sterile Hanks buffer (containing 500U / ml penicillin G, 500U / ml gentamicin). In a biological safety cabinet, wash the specimen with sterile Hanks buffer 4-5 times, and cut it into 1mm pieces with ophthalmic scissors. 3 Small pieces were digested in 1mg / ml type I collagenase / hyaluronidase at 37°C for 1 hour, vigorously shaken once every 15 minutes, filtered through a 300-mesh sieve, and inoculated into DMEM medium (containing 10ng / ml recombinant Human epidermal growth factor, 1...

Embodiment 2

[0035] Example 2 Biological characteristics and application of ZRLC-1 cells

[0036]In the present invention, as in Example 1, ZRLC-1 cells are cultured with RPMI 1640 medium containing fetal bovine serum and antibiotics, so that they can grow and pass stably in vitro for a long time. When the cells have been passed on for more than 10 generations, the cell properties are gradually stabilized, and the relevant biology, genetics and tissue source identification are carried out. Through experimental observation and verification, the cells grown in vitro have a typical epithelial-like morphology, lose contact growth inhibition, and show malignant growth. Genetic studies have confirmed that the cells are aneuploid, with severe chromosome number and structural aberrations, consistent with the genetic characteristics of malignant tumors. The ZRLC-1 cells can form tumors subcutaneously in nude mice in the order of 2*10^3, and are highly tumorigenic. The ZRLC-1 cells can provide new...

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Abstract

The invention discloses a human lung adenocarcinoma cell line and an application thereof. The human lung adenocarcinoma cell line is preserved at China Center for Type Culture Collection (CCTCC) on May 23th, 2014, in Wuhan University 430072, China. The name of a culture is human lung adenocarcinoma cell ZRLC-1; and a preservation number is CCTCC NO: C2014103. The human lung adenocarcinoma cell ZRLC-1 can be used as an experimental material in lung adenocarcinoma basic and clinical research, and can be used for establishing an animal model for occurrence, development and metastasis of lung cancers. The human lung adenocarcinoma cell line has stable characters, can be sub-cultured for a plurality of times stably, and can highly express epithelial markers such as EpCAM and E-cadherin. Flow cytometric analysis shows that EpCAM positive cells reach over 99.70%; and novel experiment material closer to the biological characteristics of clinical tumors are provided for the research of the lung cancers.

Description

Technical field [0001] The invention belongs to the field of microbial animal cell lines, and particularly relates to a human lung adenocarcinoma cell line and its application. Background technique [0002] Primary lung cancer is currently one of the most common malignant tumors. There are approximately 1.3 million new cases of lung cancer in the world every year, accounting for 12.3% of all new cancer cases, ranking first, and my country is a country with a high incidence of this disease. . Epidemiological survey data in recent years show that lung cancer is one of the cancers with the fastest rising incidence and mortality among the Chinese population. According to the results of the third national cause-of-death survey released by the Ministry of Health, lung cancer ranks first among the causes of cancer death in my country, accounting for 22.7% of all cancer deaths. Compared with the 1970s, my country's lung cancer mortality rate has increased 4.65 times, which is partic...

Claims

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Application Information

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IPC IPC(8): C12N5/09A01K67/027C12R1/91
Inventor 王凯徐霞柴守杰朱永良王晓健
Owner ZHEJIANG UNIV
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