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Human anaplastic thyroid carcinoma cell line and application thereof

A thyroid, undifferentiated technology, applied in the field of cell lines, can solve the problems of uncontrollable disease progression, lack of standard treatment mode, etc., and achieve the effect of stable cell characteristics and strong proliferation activity

Active Publication Date: 2017-12-12
ZHEJIANG CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ATC can be treated with surgery, radiotherapy, chemotherapy, targeted therapy, etc., but these treatments often cannot control the progression of the disease, and there is still a lack of standard treatment models

Method used

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  • Human anaplastic thyroid carcinoma cell line and application thereof
  • Human anaplastic thyroid carcinoma cell line and application thereof
  • Human anaplastic thyroid carcinoma cell line and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Establishment of ZJB-ATC1 cell line

[0037] 1.1 The source of the specimen:

[0038] In April 2016, a 72-year-old patient with undifferentiated thyroid cancer was taken from my hospital after surgery. The patient complained of cough, small amount of sputum, and no fever before 1 month. CT examination: multiple nodules and metastases in both lungs Considering the possible source of thyroid gland, ascending aortic aneurysm-like expansion, total tumor body: 7x 6x 4.5cm, with simple collagenization, recidivism of the surrounding fibrous tissue, and the patient’s informed consent was signed before surgery.

[0039] 1.2 Specimen processing:

[0040] Take a fresh specimen of undifferentiated thyroid cancer that has been surgically removed, rinsed with normal saline 3 times, cut off the hemorrhage and necrotic tissue, and soak in PBS (containing penicillin 500IU / mL, streptomycin 500μg / mL, amphotericin B 5μg / mL) at 4°C for 10min , The normal saline rinses the specimen 3 tim...

Embodiment 2

[0048] Example 2: Biological characteristics and application of ZJB-ATC1 cell line

[0049] a. Morphological observation of ZJB-ATC1 cell line

[0050] Take the subcultured ZJB-ATC1 cells and observe the growth of living cells (40×) under an optical microscope (Japan Olympus IMT-2 inverted microscope). See the result Figure 1A~Figure 1D , Figure 1A~Figure 1D The optical morphology pictures of the P0, P6, P17, and P50 generations of the ZJB-ATC1 cell line, respectively Figure 1A~Figure 1D Visible: The cell line grows vigorously, the background is clear, and impurities are rare. The adherent growth cells are fibrous and can be passaged in 4-5 days.

[0051] b. STR analysis of genetic material of ZJB-ATC1 cell line

[0052] Short tandem repeat (STR), also known as microsatellite DNA, refers to a type of DNA sequence formed by tandem repeats on a chromosome with several base pairs as the core unit (2-6 base pairs) (The number of repetitions is 10 to 60 times, and the gene fragment is bel...

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Abstract

The invention discloses a human anaplastic thyroid carcinoma cell line and application thereof. The human anaplastic thyroid carcinoma cell line is named as a human anaplastic thyroid carcinoma cell ZJB-ATC1 and is preserved in China Center for Type Culture Collection (CCTCC) in Wuhan University on March 31, 2017, with the preservation number of CCTCC NO: C201753. The human anaplastic thyroid carcinoma cell line disclosed by the invention can be subjected to in vitro passage for a long time, can be amplified to obtain a large quantity of offspring and has relatively strong proliferation activity; a classification type is undifferentiated and can be used as an effective study cell model and a novel experiment model is provided for researchers to deeply develop etiology, a transfer mechanism, a drug-resisting mechanism, new drug screening and the like of human anaplastic thyroid carcinoma.

Description

Technical field [0001] The invention belongs to the field of cell lines, and specifically relates to human thyroid undifferentiated cancer cell lines and applications thereof. Background technique [0002] Anaplastic thyroid cancer (Anaplastic thyroid cancer, ATC) is a solid tumor with a high degree of malignancy (fatality). Its incidence does not exceed 5% of thyroid cancer, but its mortality accounts for 50% of thyroid cancer. Deaths account for half of thyroid cancer deaths). Due to the rapid development of undifferentiated thyroid cancer, the surrounding organs, such as trachea, esophagus, blood vessels, muscles, etc. are often locally invaded at the time of diagnosis, and about 50% of patients have metastases to the lung, bone, brain, liver, etc., even if given With active treatment, only a few patients can survive for a long time. Generally, the median survival period from diagnosis to death is only 4 to 8 months. UICC and AJCC divide all ATCs into phase IV. ATC can be t...

Claims

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Application Information

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IPC IPC(8): C12N5/09C12Q1/02C12R1/91
CPCC12N5/0693G01N33/5011G01N33/5044
Inventor 刘小珍葛明华郑智国凌志强孙文勇徐海苗王佳峰
Owner ZHEJIANG CANCER HOSPITAL
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