[Delta]Tlrgt2 trichoderma engineering bacteria with high yield of peptaibols and construction method and application thereof

A construction method and technology of antimicrobial peptides, applied in the field of bioengineering, can solve the problems of unsatisfactory application of peptaibols antimicrobial peptides, high price, etc.
CN109401985AActive Publication Date: 2019-03-01SHANDONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANDONG UNIV
Publication Date
2019-03-01

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Abstract

The invention discloses [delta]Tlrgt2 trichoderma engineering bacteria with high yield of peptaibols and a construction method and application thereof. A Tlrgt2 knockout vector is constructed by upstream and downstream homologous arm sequences of a Tlrgt2 gene and a hygromycin resistance gene hph sequence, the [delta]Tlrgt2 trichoderma engineering bacteria is prepared by a gene knockout method, aTlrgt2 gene is knocked out, and the peptaibols production capacity of the bacteria can be effectively improved. The stable passage of the constructed [delta]Tlrgt2 trichoderma engineering bacteria canbe achieved, the yield of the trichoderma peptaibols can be significantly increased, and the production of the peptaibols by the [delta]Tlrgt2 trichoderma engineering bacteria is about 12 times higher than that of an original strain. The application of the trichoderma peptaibols in medicine and agriculture is facilitated.
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Description

technical field

[0001] The invention relates to a ΔTlrgt2 trichoderma engineering bacterium with high production of peptaibols antibacterial peptides, a construction method and application thereof, and belongs to the technical field of bioengineering. Background technique

[0002] Trichoderma spp. is an important fungal resource, which widely exists in soil, decayed wood, rhizosphere and phyllosphere. Trichoderma is not only an important enzyme production strain in industry, but also an important bio-control factor and plant growth-promoting factor in agricultural production. The biocontrol mechanism of Trichoderma mainly includes the following aspects: (1) Hyperparasitic effect: Trichoderma can recognize the host fungus, not only attach and wrap around the host mycelium, but also produce a variety of extracellular enzymes to dissolve the host cell wall and penetrate Mycelium absorbs nutrients; (2) Antibiotic effect: Trichoderma can produce a variety of secondary metabolite...

Claims

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