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Method for producing astragalus polysaccharide through acetobacter orientalis

A technology of Acetobacter orientalis and Astragalus polysaccharides, which is applied in the field of producing Astragalus polysaccharides with Acetobacter orientalis, can solve the problems of complex operation steps, difficult purification, long cycle, etc., and achieve simple and easy-to-control fermentation process, low price, and easy procurement Effect

Inactive Publication Date: 2016-03-23
YUZHOU TIANYUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction methods of astragalus polysaccharides commonly used in the prior art mainly include: water extraction and alcohol precipitation method, microwave extraction method, alkali extraction method, enzymatic extraction method, ultrafiltration method and ultrasonic extraction, etc., wherein water extraction and alcohol precipitation method is the most used However, these methods of directly extracting astragalus polysaccharides from Astragalus have problems such as low extraction rate, poor stability, difficult purification, complicated operation steps, and long cycle.

Method used

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Examples

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Effect test

preparation example Construction

[0021] (1) Preparation of astragalus polysaccharide fermentation medium: Weigh 5 parts of fish peptone, 10 parts of lactose, 5 parts of yeast extract, 2.5 parts of dipotassium hydrogen phosphate, 2.5 parts of potassium dihydrogen phosphate, 80 parts of astragalus powder and 1000 parts of water 500ml Erlenmeyer flask, stopper the Erlenmeyer flask with a cotton plug and put it into a sterilizer for sterilization. The temperature of the sterilizer is 121°C and sterilize for 20 minutes;

[0022] (2) Preparation of MRS broth medium: weigh 10 parts of caseinase digest, 10 parts of beef extract powder, 4 parts of yeast extract powder, 2 parts of triammonium citrate, 5 parts of sodium acetate, and 0.2 parts of magnesium sulfate heptahydrate , 0.05 parts of manganese sulfate tetrahydrate, 2 parts of potassium dihydrogen phosphate, 2 parts of glucose, 100 parts of Tween-800 parts, 1000 parts of water, add the above ingredients into water, heat and dissolve, correct the pH to 5.7±0.2, and...

Embodiment 1

[0033] A method utilizing Acetobacter orientalis to produce astragalus polysaccharides, comprising the following steps:

[0034] (1) Preparation of astragalus polysaccharide fermentation medium: Weigh 0.5g of fish peptone, 1g of lactose, 0.5g of yeast extract, 0.25g of dipotassium hydrogen phosphate, 0.25g of potassium dihydrogen phosphate, 8g of astragalus powder, 100ml of water, add In a 500ml triangular flask, plug the triangular flask with a cotton plug and put it into a sterilizer for sterilization. The temperature of the sterilizer is 121°C and sterilize for 20 minutes; the astragalus powder is crushed clean astragalus slices and passed through a No. 5 drug sieve prepared;

[0035] (2) Preparation of MRS broth medium: weigh 10 parts of caseinase digest, 10 parts of beef extract powder, 4 parts of yeast extract powder, 2 parts of triammonium citrate, 5 parts of sodium acetate, and 0.2 parts of magnesium sulfate heptahydrate , 0.05 parts of manganese sulfate tetrahydrate,...

Embodiment 2

[0041] A method utilizing Acetobacter orientalis to produce astragalus polysaccharides, comprising the following steps:

[0042](1) Preparation of astragalus polysaccharide fermentation medium: Weigh 0.5g of fish peptone, 1g of lactose, 0.5g of yeast extract, 0.25g of dipotassium hydrogen phosphate, 0.25g of potassium dihydrogen phosphate, 8g of astragalus powder, 100ml of water, add In a 500ml triangular flask, plug the triangular flask with a cotton plug and put it into a sterilizer for sterilization. The temperature of the sterilizer is 121°C and sterilize for 20 minutes; the astragalus powder is crushed clean astragalus slices and passed through a No. 5 drug sieve prepared;

[0043] (2) Preparation of MRS broth medium: weigh 10 parts of caseinase digest, 10 parts of beef extract powder, 4 parts of yeast extract powder, 2 parts of triammonium citrate, 5 parts of sodium acetate, and 0.2 parts of magnesium sulfate heptahydrate , 0.05 parts of manganese sulfate tetrahydrate, ...

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Abstract

The invention provides a method for producing astragalus polysaccharide through acetobacter orientalis. The method includes the following steps that 1, a fermentation culture medium of astragalus polysaccharide is prepared; 2, an MRS bouillon culture medium is prepared; 3, enlarged culture of cetobacter orientalis is performed; 4, a fermentation reaction is performed; 5, separation and determination are performed. The method for producing astragalus polysaccharide is economical and efficient; fermentation raw materials used in the method are low in price and easy to purchase, the fermentation process is simple and easy to control, fermentation equipment and production conditions are not specially required, equipment and production conditions of a conventional fermentation plant can be adopted for production, and industrialized mass production can be easily achieved.

Description

technical field [0001] The invention relates to the field of production methods of astragalus polysaccharides, in particular to a method for producing astragalus polysaccharides by using Acetobacter orientalis. Background technique [0002] Astragalus comes from the dried root of the leguminous plant Astragalus membranaceus or Astragalus mongolica. It is a commonly used traditional Chinese medicine. Its main component, astragalus polysaccharide, has many functions such as improving immunity, anti-virus, anti-bacterial, anti-oxidation, regulating blood sugar, protecting liver and kidney, etc. Pharmacological effect. The extraction methods of astragalus polysaccharides commonly used in the prior art mainly include: water extraction and alcohol precipitation, microwave extraction, alkali extraction, enzymatic extraction, ultrafiltration and ultrasonic extraction, etc., wherein water extraction and alcohol precipitation are the most used However, these methods of directly extra...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04C12R1/02
Inventor 郭建钊梁剑平陶蕾江红格武晓娜连战胜李春慧朱永喜董运龙柴瑞红孙美鹤
Owner YUZHOU TIANYUAN BIOTECH CO LTD
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