Preparation method of bacterial cellulose, bacterial cellulose-chitosan composite gel skincare mask and preparation method thereof

A technology of bacterial cellulose membrane and bacterial cellulose, applied in the field of bacterial cellulose, can solve the problems of not conforming to the bionic design characteristics of human face, poor skin affinity, rising production cost, etc., and achieves high cost performance, reduced production cost, The effect of high mechanical strength

Inactive Publication Date: 2021-06-01
HARBIN UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Disadvantages of traditional non-woven or gelatin masks: ①It does not conform to the bionic design characteristics of the human face, resulting in poor affinity with the skin and poor water retention; ②Low mechanical strength and poor toughness make it easy

Method used

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  • Preparation method of bacterial cellulose, bacterial cellulose-chitosan composite gel skincare mask and preparation method thereof
  • Preparation method of bacterial cellulose, bacterial cellulose-chitosan composite gel skincare mask and preparation method thereof
  • Preparation method of bacterial cellulose, bacterial cellulose-chitosan composite gel skincare mask and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0029] A kind of preparation method of bacterial cellulose is characterized in that: comprises the following steps:

[0030] S01, preparation of bacterial cellulose membrane material: use the by-product residue after soybean protein extraction as the basic raw material, referred to as soybean residue, use soybean residue to prepare soybean residue bio-fermentation medium, add 15% wood to the soybean residue bio-fermentation medium For each 100mL of soybean residue bio-fermentation medium, add 20mL of Gluconacetobacter xylinum seed solution and bitter almond enzyme, pH 5.5, culture temperature 30°C, culture time 15 days, dosage of bitter almond enzyme 300U / mL , to obtain bacterial cellulose membrane material;

[0031] S02, take the bacterial cellulose membrane material, wash it with distilled water several times (specifically 3 times), then immerse it in 0.1mol / mL NaOH solution, soak it in a water bath at 80°C, and stir it magnetically at 100rpm / min for 2h to remove the remaini...

Embodiment 2

[0071] A method for preparing bacterial cellulose, comprising the following steps:

[0072] S01, preparation of bacterial cellulose membrane material: use the by-product residue after soybean protein extraction as the basic raw material, referred to as soybean residue, use soybean residue to prepare soybean residue bio-fermentation medium, add 10% wood to the soybean residue bio-fermentation medium For each 100mL of soybean residue bio-fermentation medium, add 12mL of Gluconacetobacter xylinum seed liquid and bitter almond enzyme, pH 4.8, culture temperature 28°C, culture time 12 days, the amount of bitter almond enzyme 150U / mL , to obtain bacterial cellulose membrane material;

[0073] S02, take the bacterial cellulose membrane material, wash it with distilled water several times, then immerse it in 1mol / mL NaOH solution, soak it in a water bath at 70°C, and stir it magnetically at 90rpm / min for 3h, remove the remaining bacteria and culture medium, take it out and wash it, T...

Embodiment 3

[0083] A method for preparing bacterial cellulose, comprising the following steps:

[0084] S01, preparation of bacterial cellulose membrane material: use the by-product residue after soybean protein extraction as the basic raw material, referred to as soybean residue, use soybean residue to prepare soybean residue bio-fermentation medium, add 18% wood to the soybean residue bio-fermentation medium For each 100mL of soybean residue bio-fermentation medium, add 25mL of Gluconacetobacter xylinum seed solution and bitter almond enzyme, pH 6.0, culture temperature 35°C, culture time 18 days, dosage of bitter almond enzyme 450U / mL , to obtain bacterial cellulose membrane material;

[0085] S02, take the bacterial cellulose membrane material, wash it with distilled water several times, then immerse it in 0.5mol / mL NaOH solution, soak it in a water bath at 90°C, and stir it magnetically at 120rpm / min for 2h, remove the remaining bacteria and culture medium, take it out and wash it ,...

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Abstract

The invention discloses a preparation method of bacterial cellulose. The method comprises the following steps of: preparing a soybean residue biological fermentation culture medium by using soybean residues as a basic raw material, namely soybean residues for short, adding 10-18% of a gluconacetobacter xylinum seed solution and amygdalin into the soybean residue biological fermentation culture medium, adding 12-25 mL of a gluconacetobacter xylinum seed solution into every 100 mL of the soybean residue biological fermentation culture medium, wherein the pH is 4.8-6.0, the culture temperature is 28-35 DEG C, the culture time is 12-18 days, the use amount of amygdalin is 150-450 U/mL, and obtaining a bacterial cellulose membrane material. The invention further discloses a bacterial cellulose-chitosan composite gel skin care mask and a preparation method thereof. The mask is good in tensile strength and high in mechanical strength, has a certain inhibition effect on bacteria and fungi, and is good in biocompatibility.

Description

technical field [0001] The invention relates to a preparation method of bacterial cellulose, a bacterial cellulose-chitosan composite gel skin care mask and a preparation method thereof, belonging to the technical field of bacterial cellulose. Background technique [0002] Disadvantages of traditional non-woven or gelatin masks: ①It does not conform to the bionic design characteristics of the human face, resulting in poor affinity with the skin and poor water retention; ②Low mechanical strength and poor toughness make it easy to break and peel off;③ Non-environmentally friendly materials pollute the environment after being discarded after use; ④ Hyaluronic acid, collagen and other auxiliary materials or additives are added to the mask, which leads to an increase in production costs and is expensive. Contents of the invention [0003] The technical problem to be solved by the present invention is that the present invention provides a method for preparing bacterial cellulose...

Claims

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Application Information

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IPC IPC(8): C12P19/04A61K8/73A61K8/60A61K8/49A61K8/02A61Q19/00A61Q17/00A61Q19/02A61Q19/08
CPCA61K8/0212A61K8/498A61K8/602A61K8/731A61K8/736A61K2800/81A61K2800/82A61Q17/005A61Q19/00A61Q19/02A61Q19/08C12P19/04
Inventor 刘欣黄莉于屾李霄李禹震
Owner HARBIN UNIV OF SCI & TECH
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