The invention discloses a preparation method of modified bacterial cellulose products that are directly obtained by biosynthesis with microbial fermentation, belongs to the field of biology, and relates to preparation methods of modified bacterial cellulose products that are directly obtained by biosynthesis with microbial fermentation, in particular to the preparation of modified bacterial cellulose products that adopts the raw materials including a Gluconacetobacter xylinum with the preservation number of CGMCC No.1.1812, a mould that is a material with oxygen permeability function and a culture medium as well as the steps of strain cultivation, fermentation, washing and drying. The preparation method of the invention has simple techniques, achieves direct synthesis of the products with different shapes by utilizing microbial techniques, and has low production cost. The modified bacterial cellulose products that are prepared by the method have controllable shapes and thicknesses as well as good tensile property and biocompatibility, not only can be used as food package materials but also can be used as tissue engineering scaffold materials, and have good application prospect.