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Preparation of bacteria cellulose special-shaped product by microbial fermentation direct biosynthesis

A technology for bacterial cellulose and microbial fermentation, applied in the direction of microorganisms, microorganism-based methods, fermentation, etc., can solve problems such as increased product cost and complex process

Inactive Publication Date: 2009-07-22
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This not only complicates the process, but also increases the cost of the product

Method used

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  • Preparation of bacteria cellulose special-shaped product by microbial fermentation direct biosynthesis
  • Preparation of bacteria cellulose special-shaped product by microbial fermentation direct biosynthesis
  • Preparation of bacteria cellulose special-shaped product by microbial fermentation direct biosynthesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Gluconacetoacetobacter xylinum CGMCC No.1.1812, (purchased from China General Microorganism Culture Collection and Management Center) slanted seeds were inserted into the Erlenmeyer flask containing the seed medium, and after shaking at 160r / min at 30°C for 24h, wood Gluconoacetobacter seed is inserted in the Erlenmeyer flask that 500mLHS fermentation medium is housed with the inoculum amount of 5-20% (v / v), shakes up and pours into the hand-shaped fermentation container, after heat sealing, static at 30 ℃ After 10 days of culture, bacterial cellulose is attached to the surface of the inner wall of the container and synthesized after the fermentation. After peeling off the surface of the inner wall, the bacterial cellulose special-shaped product can be obtained. Then soak and wash in running water for 1-2d and then soak in 0.1mol / LNaOH solution for 1-2d to remove the culture medium and bacteria, and wash to neutrality (pH value about 7.2), such as image 3 As shown, th...

Embodiment 2

[0031] Bacterial classification and seed culture are the same as embodiment 1, and the inoculation amount of 5-20% (v / v) of Acetobacter xylinum seed is inserted in the Erlenmeyer flask that 300mLHS fermentation medium is housed, shakes up and pours into W-shaped In the fermentation container, after sealing, culture at 30°C for 10 days. After the fermentation, bacterial cellulose adheres to the surface of the inner wall of the container to synthesize. After peeling off the surface of the inner wall, put the special-shaped bacterial cellulose product in running water for 1-2 days, soak it in 0.1mol / L NaOH solution for 1-2 days to remove the culture medium and bacteria, and wash until neutral (pH value is about 7.2 ) and freeze-dried.

Embodiment 3

[0033]Bacterial classification and seed culture are the same as embodiment 1, and the inoculum size of 5-20% (v / v) of gluconoacetoacetobacter xylinum seed is inserted in the triangular flask that 300mLHS fermentation medium is housed, shakes up and pours into ring In the shaped fermentation container, after sealing, it was cultured at 30°C for 10 days. After the fermentation, the bacterial cellulose was attached to the surface of the inner wall of the container and synthesized. After peeling off the surface of the inner wall, put the special-shaped bacterial cellulose product in running water for 1-2 days, soak it in 0.1mol / L NaOH solution for 1-2 days to remove the culture medium and bacteria, and wash until neutral (pH value is about 7.2 ), and then freeze-dried, the results are as Figure 4 shown.

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Abstract

The invention discloses a preparation method of modified bacterial cellulose products that are directly obtained by biosynthesis with microbial fermentation, belongs to the field of biology, and relates to preparation methods of modified bacterial cellulose products that are directly obtained by biosynthesis with microbial fermentation, in particular to the preparation of modified bacterial cellulose products that adopts the raw materials including a Gluconacetobacter xylinum with the preservation number of CGMCC No.1.1812, a mould that is a material with oxygen permeability function and a culture medium as well as the steps of strain cultivation, fermentation, washing and drying. The preparation method of the invention has simple techniques, achieves direct synthesis of the products with different shapes by utilizing microbial techniques, and has low production cost. The modified bacterial cellulose products that are prepared by the method have controllable shapes and thicknesses as well as good tensile property and biocompatibility, not only can be used as food package materials but also can be used as tissue engineering scaffold materials, and have good application prospect.

Description

technical field [0001] The invention belongs to the field of biology, and relates to a method for preparing a bacterial cellulose special-shaped product, in particular to a method for preparing a bacterial cellulose special-shaped product directly biosynthesized by microbial fermentation, and in particular to a method using Gluconacetobacter xylinum (Gluconacetobacterxylinum) CGMCC No.1.1812, which has The material with oxygen permeation function is a method for preparing bacterial cellulose special-shaped products by using the mold and the culture medium as raw materials through strain cultivation, fermentation, washing and drying. Background technique [0002] Bacterial cellulose (bacterial cellulose) is a polymer compound synthesized by some bacteria with an ultra-fine network structure. Although bacterial cellulose and cellulose produced by plants or seaweed have the same molecular structure unit, bacterial cellulose has many Unique properties such as high crystallinity,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/02
Inventor 贾士儒朱会霞杨洪江谭之磊贾原媛张桂才李飞
Owner TIANJIN UNIV OF SCI & TECH
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