A nano microbeam cellulose, method for preparing the same and use thereof

A microbial cellulose and nanotechnology, applied in the direction of bacteria, fermentation, and medical raw materials derived from bacteria, etc., can solve problems such as difficult classification, unevenness, and wide particle size distribution of nanocellulose particles

Inactive Publication Date: 2003-12-31
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regrettably, the nanocellulose materials prepared by physical or chemical methods often have problems such as wide particle size distribution, uneven particle size distribution, and difficult classification.

Method used

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  • A nano microbeam cellulose, method for preparing the same and use thereof
  • A nano microbeam cellulose, method for preparing the same and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Mix 500 grams of coconut water, 3000 grams of pineapple juice, 300 grams of sucrose, 6180 grams of tap water, 5 grams of potassium dihydrogen phosphate and 15 grams of ammonium sulfate to form a solution, adjust the pH value of the solution to 3, sterilize at 110 ° C, cool to 30 ℃, add Acetobacter xylinum CGMCC0603 strain, adjust the pH value to 3 every 2 hours in the incubator, the gas-liquid ratio is 30% (volume ratio), cultivate for 48 hours, and then separate and purify to make nano-microbial fibers Vegetarian 10 mg. Its particle size is 100 nm as tested by electron microscopy (see figure 2 ).

Embodiment 2

[0021] Mix 5000 grams of coconut water, 800 grams of glucose, 4130 grams of tap water, 15 grams of potassium dihydrogen phosphate and 55 grams of ammonium sulfate to form a solution, adjust the pH value of the solution to 6, sterilize at 110 ° C, cool to 25 ° C, add wood vinegar The bacillus CGMCC0603 strain is adjusted to a pH value of 6 every 2 hours in an incubator, and the gas-liquid ratio is 48% (volume ratio), cultivated for 12 hours, and then separated and purified to produce 30 mg of nano-microbial cellulose.

Embodiment 3

[0023] Mix 5000 grams of apple juice, 300 grams of fructose, 4670 grams of tap water, 5 grams of potassium dihydrogen phosphate and 25 grams of ammonium sulfate to form a solution, adjust the pH value of the solution to 6, sterilize with microwave, cool to 34 ° C, add Acetobacter xylinum The C6MCC0603 bacterial strain is adjusted to a pH value of 6 every 2 hours in an incubator, and the gas-liquid ratio is 56% (volume ratio), cultivated for 24 hours, and then separated and purified to produce 8 mg of nano-microbial cellulose.

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Abstract

The present invention relates to one kind of nano microbial cellulose and the nano microbial cellulose features the size range of 10-1000 nm. The present invention also relates to the preparation process of the nano microbial cellulose. On the other hand, the present invention also relates to the use of the nano microbial cellulose in preparing medicine composition.

Description

field of invention [0001] The invention relates to a nano-microbial cellulose, wherein the nano-microbial cellulose is characterized in that its particle size is in the range of 10-1000 nanometers. The invention also relates to a method for preparing the nano microbial cellulose. Another aspect of the present invention relates to the use of the nano-microbial cellulose for preparing a pharmaceutical composition. Background of the invention [0002] Fruit juices such as coconut water and apple juice are rich in nutrients and can be drunk directly. In addition, the fruit juice can also be used to produce microbial cellulose gel, which can be used in food, beverage and other industries. As Byrom et al. (EP0279506A1) reported that Acetobacter xylinum (Acetobacter xylinum) acts on a specific medium to prepare extracellular microbial cellulose, Brown et al. (EP0258038A2) also reported the use of specific microorganisms to prepare microbial cellulose method. But ordinary microb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74C12N1/20C12P19/04
Inventor 裴重华林强吴周新王锡彬孙中亮艾亚菲庞素娟
Owner HAINAN UNIVERSITY
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