Bioreactor for producing microbial cellulose and method thereof

A microbial cellulose and bioreactor technology, applied in the direction of microorganism-based methods, specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, etc., can solve problems such as production efficiency and unsatisfactory molds

Active Publication Date: 2010-12-22
FOOD IND RES & DEV INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the existing methods and molds in the above-mentioned patents are used to produce tubular microbial cellulose membranes, the production efficiency and the molds used can be further improved
In particular, when the size of the tubular microbial cellu

Method used

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  • Bioreactor for producing microbial cellulose and method thereof
  • Bioreactor for producing microbial cellulose and method thereof
  • Bioreactor for producing microbial cellulose and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: A single set of three hollow tubes fitted together

[0056] This example uses figure 1 and figure 2 In the bioreactor shown, the vessel 30 has dimensions of 33 cm long x 23 cm wide x 4 cm high, and the three hollow tubes 11-13 have a length of 30 cm. A motor drives the rotating shaft 22 to rotate at a speed of 10 rpm, that is, the horizontal mold set 10 rotates horizontally at a speed of 10 rpm.

[0057] The bioreactor is placed in an environment free of bacteria contamination for microbial cultivation, wherein the height of the microbial cultivation liquid medium 40 in the container 30 is 35mm, and the cultivation temperature is 30°C. The culture liquid medium 40 is pre-prepared shaking culture liquid. The pre-oscillation culture solution is a liquid medium containing the ingredients listed in the following table and a bacterial cell concentration of 5%, which is filled with xylogluconate obtained by culturing at 120rpm and 30°C for two days under the co...

Embodiment 2

[0061] Embodiment 2: a single set of two nested hollow tubes

[0062] This embodiment repeats the steps of Example 1 and the bioreactor used, except that the second hollow tube (diameter 40 mm) of the three hollow tubes is not used.

[0063] The present embodiment obtains 1.745g / L sheet-like bacterial cellulose in the undisturbed part, while the horizontal mold set part obtains a total of two tubular bacterial cellulose of 1.815g / L, and the total output is 3.56g / L.

Embodiment 3

[0064] Embodiment 3: Single hollow tube

[0065] This embodiment repeats the steps of Example 1 and the bioreactor used, except that the first hollow tube (diameter 30mm) and the second hollow tube (diameter 40mm) of the three hollow tubes are not used. .

[0066] The present embodiment obtains 1.745g / L flake bacterial cellulose body in the undisturbed part, and a tubular bacterial cellulose body of 0.77g / L is obtained in the horizontal mold set part, and the total output is 2.515g / L.

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Abstract

The invention relates to a bioreactor for producing microbial cellulose and a method thereof. The method comprises the following steps: preparing a microbial culture liquid medium in a container; horizontally rotating a plurality of hollow tubes which are alternately sheathed together or separated from each other so that each part of each of the hollow tubes is immersed in the liquid medium held in the container and exposed on a horizontal plane of the liquid medium in turn, wherein, each hollow tube is provided with a rough outer surface and a smooth inner surface, so a microbe forms the microbial cellulose on the outer surface of each hollow tube and forms the flaky microbial cellulose on the horizontal plane part of the liquid medium which is not disturbed by the plurality of the hollow tubes in the container; and removing the microbial cellulose from the outer surface of each hollow tube to obtain the tubular microbial cellulose, and obtaining the flaky microbial cellulose from the liquid medium.

Description

technical field [0001] The present invention relates to a bioreactor and method for producing microbial cellulose, in particular to a bioreactor and method capable of simultaneously producing tubular microbial cellulose and sheet microbial cellulose. Background technique [0002] Certain bacteria, especially Acetobacter, can be cultured to produce bacterial cellulose. Acetobacter synthesizes cellulose extracellularly in the presence of oxygen and sugar, which attaches to the cells in the form of fibers. The fibers produced by cells cultured in a static medium intertwine to form a hydrophilic network called a pellicle. The pellicle forms the air / culture fluid interface in still and undisturbed culture fluid. Microbial cellulose membranes have many uses after removing cells, such as wound dressings, paper making, cosmetic care products, and acoustic diaphragms. [0003] Traditionally, static culture is used to produce bacterial cellulose membranes, but static culture requir...

Claims

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Application Information

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IPC IPC(8): C12M1/10C12P19/04C12R1/02
CPCC12M23/44C12M27/12C12M23/06
Inventor 萧旭洲卢廷升陈汉根赖进此李福临朱中亮廖启成
Owner FOOD IND RES & DEV INST
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