Moisturizing eye mask prepared from bacterial cellulose
A bacterial cellulose film and bacterial cellulose technology, applied in the field of new eye masks, can solve the problems of easy moisture volatilization, inconvenient carrying, poor water holding performance, etc., and achieve non-irritating, no foreign body rejection, and high water absorption Effect
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Embodiment 1
[0037] A kind of moisturizing eye mask that utilizes bacterial cellulose to prepare, its preparation process specifically comprises the following steps:
[0038] (1) Strain selection
[0039] Select Acetobacter xylinum ( Gluconacetobacter xylinum ) CGMCC No.1.1812;
[0040] (2) Culture medium preparation
[0041] Calculated by weight percentage, containing 2% fructose, 0.5% yeast powder, 0.5% tryptone, 0.5% Na 2 HPO 4, 0.2% citric acid, 2% calcium carbonate, the balance is deionized water, add 0.4g of β-polymalic acid per liter, then use citric acid to adjust the pH value of the medium to 6, at a temperature of 121 ° C Sterilize under high temperature for 20 minutes, cool to 30°C, and set aside;
[0042] (3) Seed cell culture
[0043] Pick a ring of Acetobacter xylinum ( Gluconacetobacter xylinum ) CGMCC No.1.1812 or Acetobacter xylinum ( Gluconacetobacter xylinum ) The strain CGMCC No.1.2378 was inoculated in the slant culture medium, cultivated in a constant temperat...
Embodiment 2
[0052] A kind of moisturizing eye mask that utilizes bacterial cellulose to prepare, its preparation process specifically comprises the following steps:
[0053] (1) Strain selection
[0054] Select Acetobacter xylinum ( Gluconacetobacter xylinum ) CGMCC No.1.2378.
[0055] (2) Culture medium preparation
[0056] In terms of weight percentage, containing 4% sucrose, 1% yeast powder, 1% tryptone, 1% Na 2 HPO 4 , 0.3% citric acid, 3% calcium carbonate, the balance is deionized water, add 0.3g / L β-polymalic acid per liter, and then adjust the pH value of the medium to 5.3 with glacial acetic acid, at 121 Sterilize at a temperature of ℃ for 20 minutes, cool to 30 ℃, and set aside;
[0057] (3) Seed cell culture
[0058] Pick a ring of Acetobacter xylinum ( Gluconacetobacter xylinum ) The strain CGMCC No.1.2378 was inoculated in the slant culture medium, cultivated in a constant temperature incubator at 28-32°C for 72 hours, and used as activated slant seeds;
[0059] The s...
Embodiment 3
[0067] A kind of moisturizing eye mask that utilizes bacterial cellulose to prepare, its preparation process specifically comprises the following steps:
[0068] (1) Strain selection
[0069] Select Acetobacter xylinum ( Gluconacetobacter xylinum ) CGMCC No.1.1812.
[0070] (2) Culture medium preparation
[0071] Calculated by weight percentage, containing 3% glucose, 0.8% yeast powder, 0.7% tryptone, 0.8% Na 2 HPO 4 , 0.25% citric acid, 2.5% calcium carbonate, the balance is deionized water, add 0.5g of β-polymalic acid per liter, and then use citric acid to adjust the pH value of the medium to 5.7, at a temperature of 121 ° C Sterilize under high temperature for 20 minutes, cool to 30°C, and set aside;
[0072] (3) Seed cell culture
[0073] Pick a ring of Acetobacter xylinum ( Gluconacetobacter xylinum ) The CGMCC No.1.1812 strain was inoculated in the slant medium, cultivated in a constant temperature incubator at 28-32°C for 72 hours, and used as the activated slan...
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