Bacterial cellulose fermentation medium made from apple pomace and method for producing bacterial cellulose by utilizing medium

A technology for bacterial cellulose and fermentation medium, applied in the biological field, can solve the problems of low yield and yield, high cost of bacterial cellulose medium, and achieve the effects of improving yield, good solubility, and improving hydrolysis efficiency

Active Publication Date: 2014-09-10
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problems of high cost, low yield and yield of bacterial cellulose medium are the bottlenecks of its industrial production and popularization and application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The bacterial strain described in the present invention: Gluconacetobacter xylinus, purchased from China Agricultural Microorganism Culture Collection and Management Center, strain number accc10215.

[0028] A method for producing bacterial cellulose, comprising the steps of:

[0029] (1) Activation of Gluconacetobacter xylosus

[0030] Prepare gluconacetobacter xylosus activation medium, the formula of activation medium includes sucrose 40g / L, beef extract 10g / L, disodium hydrogen phosphate 4g / L, citric acid 0.8g / L, ethanol 8g / L, agar 15g / L, adjust the pH value to 5.5. Inoculate Gluconacetobacter xylosus accc10215 in the activation medium, culture at 28°C for 28 hours, inoculate the cultured bacteria again in the same, new activation medium, and cultivate at 28°C for 28 hours to obtain activated wood Gluconacetobacter sucrose;

[0031] (2) Expansion of Gluconacetobacter xylosus

[0032] Prepare gluconacetobacter xylosus expansion culture medium, the formula of expa...

Embodiment 2

[0041] A method for producing bacterial cellulose, comprising the steps of:

[0042] (1) Activation of Gluconacetobacter xylosus

[0043] Prepare gluconacetobacter xylosus activation medium, the formula of activation medium comprises sucrose 45g / L, beef extract 13g / L, disodium hydrogen phosphate 4.5g / L, citric acid 0.9g / L, ethanol 9g / L, Agar 17g / L, adjust the pH value to 6.0. Inoculate Gluconacetobacter xylosus accc10215 in the activation medium, culture at 28°C for 28 hours, inoculate the cultured bacteria again in the same, new activation medium, and cultivate at 28°C for 28 hours to obtain activated wood Gluconacetobacter sucrose;

[0044] (2) Expansion of Gluconacetobacter xylosus

[0045] Prepare gluconacetobacter xylosus expansion culture medium, the formula of expansion culture medium comprises sucrose 45g / L, beef extract 13g / L, disodium hydrogen phosphate 4.5g / L, citric acid 0.9g / L, ethanol 9g / L L, adjusting the pH value to 6.0, inoculating activated Gluconacetobac...

Embodiment 3

[0054] A method for producing bacterial cellulose, comprising the steps of:

[0055] (1) Activation of Gluconacetobacter xylosus

[0056] Prepare Gluconacetobacter xylosus activation medium, the formula of activation medium includes sucrose 50g / L, beef extract 15g / L, disodium hydrogen phosphate 5g / L, citric acid 1.0g / L, ethanol 10g / L, agar 20g / L, adjust the pH value to 6.5. Inoculate Gluconoacetobacter xylosus accc10215 in the activation medium, culture at 32°C for 32 hours, inoculate the cultured bacteria again in the same, new activation medium, and cultivate at 32°C for 32 hours to obtain activated wood Gluconacetobacter sucrose;

[0057] (2) Expansion of Gluconacetobacter xylosus

[0058] Prepare gluconacetobacter xylosus expansion culture medium, the formula of expansion culture medium includes sucrose 50g / L, beef extract 15g / L, disodium hydrogen phosphate 5g / L, citric acid 1.0g / L, ethanol 10g / L , adjust the pH value to 6.5, inoculate the activated gluconacetobacter x...

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PUM

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Abstract

The invention discloses a bacterial cellulose fermentation medium made from apple pomace and a method for producing the bacterial cellulose by utilizing the medium, and belongs to the field of biotechnology. The bacterial cellulose fermentation medium comprises cane sugar, beef extract, disodium hydrogen phosphate, citric acid, ethanol and apple pomace hydrolysate. The method comprises the following steps: (1) activating and propagating gluconacetobacter xylinum to obtain gluconacetobacter xylinum seed solution, incubating the gluconacetobacter xylinum seed solution into a bacterial cellulose fermenting medium for fermenting to obtain bacterial cellulose fermenting solution; and (2) treating a bacterial cellulose in the bacterial medium fermenting solution to obtain bacterial cellulose. The method has the advantages of low cost, simple process and the like, is convenient for industrial production, is good in stability and reproducibility, and is suitable for large-scale production, wherein the yield of the fermented bacterial cellulose is more than 15g/L.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a bacterial cellulose fermentation medium using apple pomace as a raw material and a method for producing bacterial cellulose using the medium. Background technique [0002] Bacterial cellulose is produced by bacteria growing in a liquid sugar-containing matrix and secreted into the matrix. It is a high molecular polymer composed of glucose linked by β-1,4 glycosidic bonds. It is used in biomedicine Materials, pharmaceutical materials, pervaporation membranes, fuel cells, papermaking and its cellulose derivatives have broad application prospects. However, the problems of high cost, low yield and yield of bacterial cellulose medium are the bottlenecks of its industrial production and popularization and application. Utilizing various waste residues and waste liquids to produce bacterial cellulose and reduce its production cost is an effective way to promote its industrial ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/02
Inventor 王学川张雯刘兰高媛李彦军鬲万云
Owner SHAANXI UNIV OF SCI & TECH
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