The invention relates to the technical field of edible fungus cultivation, in particular to an edible fungus cultivation base material and a preparation method thereof. The edible fungus cultivation base material is prepared from the following raw materials in parts by weight: 10-20 parts of Chinese yam residues, 5-10 parts of pineapple residues, 8-16 parts of fungus residues, 3-8 parts of microcystin, 6-9 parts of agar and 3-5 parts of compound sodium nitrophenolate. According to the edible fungus cultivation base material, the Chinese yam residues, the fungus residues and the pineapple residues are utilized, the raw material consumption of the cultivation base material in a traditional technology is reduced, the preparation cost of the edible fungus cultivation base material is saved, meanwhile, the problems that food processing leftovers are improperly treated, and consequently environmental pollution is caused are solved, waste is turned into wealth, and economic benefits are high;meanwhile, no harmful substance is added in the cultivation base material from the formula to the production process, the obtained base material is scientific and reasonable, the preparation method is simple, and sufficient and reasonable nutrients can be provided for growth of edible fungi.