A bacterial cellulose fermentation medium using apple pomace as raw material and a method for producing bacterial cellulose using the medium

Abstract

The invention discloses a bacterial cellulose fermentation medium using apple pomace as a raw material and a method for producing bacterial cellulose by using the medium, belonging to the field of biotechnology. The bacterial cellulose fermentation medium of the present invention contains sucrose, beef extract, disodium hydrogen phosphate, citric acid, ethanol and apple pomace hydrolyzate. The method for producing bacterial cellulose using the culture medium includes: 1) activating and expanding the gluconacetobacter xylosus to obtain the gluconacetobacter xylosacillus seed solution, and the gluconacetobacter xylosus seed solution Inserting bacterial cellulose fermentation medium for fermentation to obtain bacterial cellulose fermentation liquid; 2) taking the bacterial cellulose film in the bacterial cellulose fermentation liquid, and obtaining bacterial cellulose after treatment. The method has the advantages of low cost, simple process, convenient industrial production, etc., good stability and reproducibility, the yield of bacterial cellulose obtained by fermentation is greater than 15g / L, and is suitable for large-scale production.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a bacterial cellulose fermentation medium using apple pomace as a raw material and a method for producing bacterial cellulose using the medium. Background technique

[0002] Bacterial cellulose is produced by bacteria growing in a liquid sugar-containing matrix and secreted into the matrix. It is a high molecular polymer composed of glucose linked by β-1,4 glycosidic bonds. It is used in biomedicine Materials, pharmaceutical materials, pervaporation membranes, fuel cells, papermaking and its cellulose derivatives have broad application prospects. However, the problems of high cost, low yield and yield of bacterial cellulose medium are the bottlenecks of its industrial production and popularization and application. Utilizing various waste residues and waste liquids to produce bacterial cellulose and reduce its production cost is an effective way to promote its industrial ...

Examples

Embodiment 1

[0027] The bacterial strain described in the present invention: Gluconacetobacter xylinus, purchased from China Agricultural Microorganism Culture Collection and Management Center, strain number accc10215.

[0028] A method for producing bacterial cellulose, comprising the steps of:

[0029] (1) Activation of Gluconacetobacter xylosus

[0030] Prepare gluconacetobacter xylosus activation medium, the formula of activation medium includes sucrose 40g / L, beef extract 10g / L, disodium hydrogen phosphate 4g / L, citric acid 0.8g / L, ethanol 8g / L, agar 15g / L, adjust the pH value to 5.5. Inoculate Gluconacetobacter xylosus accc10215 in the activation medium, culture at 28°C for 28 hours, inoculate the cultured bacteria again in the same, new activation medium, and cultivate at 28°C for 28 hours to obtain activated wood Gluconacetobacter sucrose;

[0031] (2) Expansion of Gluconacetobacter xylosus

[0032] Prepare gluconacetobacter xylosus expansion culture medium, the formula of expa...

Embodiment 2

[0041] A method for producing bacterial cellulose, comprising the steps of:

[0042] (1) Activation of Gluconacetobacter xylosus

[0043] Prepare gluconacetobacter xylosus activation medium, the formula of activation medium comprises sucrose 45g / L, beef extract 13g / L, disodium hydrogen phosphate 4.5g / L, citric acid 0.9g / L, ethanol 9g / L, Agar 17g / L, adjust the pH value to 6.0. Inoculate Gluconacetobacter xylosus accc10215 in the activation medium, culture at 28°C for 28 hours, inoculate the cultured bacteria again in the same, new activation medium, and cultivate at 28°C for 28 hours to obtain activated wood Gluconacetobacter sucrose;

[0044] (2) Expansion of Gluconacetobacter xylosus

[0045] Prepare gluconacetobacter xylosus expansion culture medium, the formula of expansion culture medium comprises sucrose 45g / L, beef extract 13g / L, disodium hydrogen phosphate 4.5g / L, citric acid 0.9g / L, ethanol 9g / L L, adjusting the pH value to 6.0, inoculating activated Gluconacetobac...

Embodiment 3

[0054] A method for producing bacterial cellulose, comprising the steps of:

[0055] (1) Activation of Gluconacetobacter xylosus

[0056] Prepare Gluconacetobacter xylosus activation medium, the formula of activation medium includes sucrose 50g / L, beef extract 15g / L, disodium hydrogen phosphate 5g / L, citric acid 1.0g / L, ethanol 10g / L, agar 20g / L, adjust the pH value to 6.5. Inoculate Gluconoacetobacter xylosus accc10215 in the activation medium, culture at 32°C for 32 hours, inoculate the cultured bacteria again in the same, new activation medium, and cultivate at 32°C for 32 hours to obtain activated wood Gluconacetobacter sucrose;

[0057] (2) Expansion of Gluconacetobacter xylosus

[0058] Prepare gluconacetobacter xylosus expansion culture medium, the formula of expansion culture medium includes sucrose 50g / L, beef extract 15g / L, disodium hydrogen phosphate 5g / L, citric acid 1.0g / L, ethanol 10g / L , adjust the pH value to 6.5, inoculate the activated gluconacetobacter x...

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a bacterial cellulose fermentation medium using apple pomace as a raw material and a method for producing bacterial cellulose using the medium. Background technique

[0002] Bacterial cellulose is produced by bacteria growing in a liquid sugar-containing matrix and secreted into the matrix. It is a high molecular polymer composed of glucose linked by β-1,4 glycosidic bonds. It is used in biomedicine Materials, pharmaceutical materials, pervaporation membranes, fuel cells, papermaking and its cellulose derivatives have broad application prospects. However, the problems of high cost, low yield and yield of bacterial cellulose medium are the bottlenecks of its industrial production and popularization and application. Utilizing various waste residues and waste liquids to produce bacterial cellulose and reduce its production cost is an effective way to promote its industrial ...

Examples

Embodiment 1

[0027] The bacterial strain described in the present invention: Gluconacetobacter xylinus, purchased from China Agricultural Microorganism Culture Collection and Management Center, strain number accc10215.

[0028] A method for producing bacterial cellulose, comprising the steps of:

[0029] (1) Activation of Gluconacetobacter xylosus

[0030] Prepare gluconacetobacter xylosus activation medium, the formula of activation medium includes sucrose 40g / L, beef extract 10g / L, disodium hydrogen phosphate 4g / L, citric acid 0.8g / L, ethanol 8g / L, agar 15g / L, adjust the pH value to 5.5. Inoculate Gluconacetobacter xylosus accc10215 in the activation medium, culture at 28°C for 28 hours, inoculate the cultured bacteria again in the same, new activation medium, and cultivate at 28°C for 28 hours to obtain activated wood Gluconacetobacter sucrose;

[0031] (2) Expansion of Gluconacetobacter xylosus

[0032] Prepare gluconacetobacter xylosus expansion culture medium, the formula of expa...

Embodiment 2

[0041] A method for producing bacterial cellulose, comprising the steps of:

[0042] (1) Activation of Gluconacetobacter xylosus

[0043] Prepare gluconacetobacter xylosus activation medium, the formula of activation medium comprises sucrose 45g / L, beef extract 13g / L, disodium hydrogen phosphate 4.5g / L, citric acid 0.9g / L, ethanol 9g / L, Agar 17g / L, adjust the pH value to 6.0. Inoculate Gluconacetobacter xylosus accc10215 in the activation medium, culture at 28°C for 28 hours, inoculate the cultured bacteria again in the same, new activation medium, and cultivate at 28°C for 28 hours to obtain activated wood Gluconacetobacter sucrose;

[0044] (2) Expansion of Gluconacetobacter xylosus

[0045] Prepare gluconacetobacter xylosus expansion culture medium, the formula of expansion culture medium comprises sucrose 45g / L, beef extract 13g / L, disodium hydrogen phosphate 4.5g / L, citric acid 0.9g / L, ethanol 9g / L L, adjusting the pH value to 6.0, inoculating activated Gluconacetobac...

Embodiment 3

[0054] A method for producing bacterial cellulose, comprising the steps of:

[0055] (1) Activation of Gluconacetobacter xylosus

[0056] Prepare Gluconacetobacter xylosus activation medium, the formula of activation medium includes sucrose 50g / L, beef extract 15g / L, disodium hydrogen phosphate 5g / L, citric acid 1.0g / L, ethanol 10g / L, agar 20g / L, adjust the pH value to 6.5. Inoculate Gluconoacetobacter xylosus accc10215 in the activation medium, culture at 32°C for 32 hours, inoculate the cultured bacteria again in the same, new activation medium, and cultivate at 32°C for 32 hours to obtain activated wood Gluconacetobacter sucrose;

[0057] (2) Expansion of Gluconacetobacter xylosus

[0058] Prepare gluconacetobacter xylosus expansion culture medium, the formula of expansion culture medium includes sucrose 50g / L, beef extract 15g / L, disodium hydrogen phosphate 5g / L, citric acid 1.0g / L, ethanol 10g / L , adjust the pH value to 6.5, inoculate the activated gluconacetobacter x...