Seed tissue culture medium for root of subprostrate sophora

Abstract

The invention provides a seed tissue culture medium for the root of subprostrate sophora, and relates to the technical field of tissue culture. The seed tissue culture medium is formed by mixing a basal culture solution and a mother solution with the volume ratio being 3-5:0.03-0.08. The mother solution mainly contains hymexazol, brassinolide, alpha-naphthylacetic sodium salt, pyrimethanil and diethyl aminoethyl hexanoate. The basal culture solution mainly contains pineapple waste hydrolysate, cane sugar, agar, lactoalbumin hydrolysate, ABT rooting powder, zeatin, kinetin, isopentenylaminopurine and gibberellin. According to the seed tissue culture medium disclosed by the invention, the using quantity of culture medium raw materials like saccharose in a traditional process is reduced through utilization of pineapple waste in the basal culture solution, and preparation cost of the culture medium is reduced. In addition, by adding the formula of the mother solution, rooting of strong seedlings can be promoted quickly, and the rooting percentage can reach 99% or above. Moreover, the average plant number reaches 20-25 after the strong seedlings take roots.

Description

[0001] 【Technical field】 [0002] The invention relates to the technical field of tissue culture, in particular to a broad bean root seed tissue culture medium. [0003] 【Background technique】 [0004] Broad bean root, also known as mountain bean root, is the root of the leguminous plant Vietnam Sophora japonica. It has the effect of clearing heat and detoxifying, relieving throat and detumescence. It can be used to treat sore throat, swollen gums, lung heat cough, damp-heat jaundice, nasopharyngeal cancer, cervical cancer and other diseases. Modern research has found that the total alkali contained in broad bean root and oxidation Matrine has the functions of promoting coronary flow, inhibiting tumor cells and improving immunity. [0005] Broad bean root is mainly distributed in Guangxi, Yunnan and Guizhou are also produced, but the output is small. It mainly comes from the wild. After years of excavation, wild resources are decreasing year by year. Some producing areas are ...

AI Technical Summary

Problems solved by technology

It mainly comes from the wild. After years of excavation, wild resources are decreasing year by year. Some producing areas are on the verge of being exhausted or are in a state of exhaustion. The distribution area is shrinking day by day, and the market volume is decreasing year by year. increasing year by year, resulting in a serious shortage of supply

[0006] Under the natural state, the broad bean root mainly relies on seeds for breeding. However, most of the seeds often fall off before they are mature, resulting in a low reproduction coefficient. In addition, it is obviously difficult to artificially raise seedlings by taking seeds. Broad bean root seeds are the provenance, shortening the seedling time, ensuring the survival rate of the buds after germination, increasing the thickness of the sprouts, and ensuring the cultivation methods of the dark green buds after germination are imminent