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Monstera deliciosa tissue culture method

A technology of tissue culture and monstera, which is applied in the field of plant cultivation and reproduction, can solve the problems of cutting propagation difficult to meet large-scale production, waste of branches, underdeveloped root system, etc., achieve good economic and ecological benefits, convenient material acquisition, and low cost Effect

Active Publication Date: 2018-06-22
贵港沃斯顿科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] During the cultivation process, monstera is usually propagated by seeds, and the offspring vary greatly. Cutting propagation and tiller propagation can maintain the excellent characteristics of the female parent to the greatest extent, and the production cost is relatively low. Especially in industrial poverty alleviation projects, cutting propagation is difficult to meet the needs of large-scale production in a short period of time, and it also has disadvantages such as underdeveloped root system and waste of branches. It is of great significance to meet the large-scale production of monstera tissue culture technology

Method used

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  • Monstera deliciosa tissue culture method

Examples

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Embodiment 1

[0029] A kind of method of monstera tissue culture provided by the present embodiment comprises the following steps:

[0030] (1) Pretreatment of explants: cut the stem top or axillary buds of Monstera as explants, cut the explants into 4cm strips, then soak the explants for 3s with 70% alcohol, and then Soak in 1% sodium hypochlorite solution for 15 minutes, oscillate continuously during the soaking process, rinse the explants with sterile water 4 times after soaking, then place them on a sterile inoculation tray to dry the surface moisture, cut off the leaves and old Incision, cut into 2 cm stems with 2 buds, rinse the buds with tap water for 30 minutes, and dry the buds with filter paper for later use;

[0031] (2) Cultivation of medium:

[0032] (1) Induction culture: inoculate the budded stem segments obtained in the above steps on the induction medium, and cultivate them in the dark for 5 days, and then carry out the induction culture alternately according to the light ...

Embodiment 2

[0038] A kind of method of monstera tissue culture provided by the present embodiment comprises the following steps:

[0039] (1) Pretreatment of explants: cut the stem top or axillary buds of Monstera as explants, cut the explants into 5cm strips, then soak the explants for 4s with 70% alcohol, and then Soak in 1.2% sodium hypochlorite solution for 22 minutes, oscillate continuously during the soaking process, rinse the explants with sterile water 5 times after soaking, then place them on a sterile inoculation tray to dry the surface moisture, cut off the leaves and old Incision, cut into 2.2cm stem section with 2 buds, rinse the bud stem section under tap water for 30 minutes, dry the bud stem section with filter paper for later use;

[0040] (2) Cultivation of medium:

[0041] (1) Induction culture: inoculate the budded stem segment obtained in the above steps on the induction medium, and cultivate it under dark conditions for 7 days, and then carry out induction culture a...

Embodiment 3

[0047] A kind of method of monstera tissue culture provided by the present embodiment comprises the following steps:

[0048] (1) Pretreatment of explants: cut the stem top or axillary buds of Monstera as explants, cut the explants into 6cm strips, then soak the explants for 5s with 70% alcohol, and then Soak in 1.5% sodium hypochlorite solution for 30 minutes, oscillate continuously during the soaking process, rinse the explants with sterile water 6 times after soaking, then place them on a sterile inoculation tray to dry the surface moisture, cut off the leaves and old Incision, cut into 2.5cm stems with 3 buds, rinse the stems with buds under tap water for 30 minutes, dry the stems with buds with filter paper for later use;

[0049] (2) Cultivation of medium:

[0050] (1) Induction culture: inoculate the budded stem section obtained in the above steps on the induction medium, and cultivate it under dark conditions for 10 days, then carry out induction culture alternately a...

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Abstract

The invention provides a monstera deliciosa tissue culture method, and relates to the technical field of plant culture breeding. The method comprises the following steps of (1) explant pre-treatment;(2) culture medium culture; (3) greenhouse seedling domestication; (4) rooted seedling transplanting. According to the monstera deliciosa tissue culture method, the callus tissues of cedarwood are induced onto an MS culture medium with different hormone proportioning ratios; through the culture of an induced culture medium, a proliferation culture medium and a rooting culture medium, each culturemedium provides factors required by explants in the rooting and seedling strengthening period; good conditions can be provided for rooting; the seedlings are promoted to be healthy and strong. The culture method has the advantages that the material taking is convenient; the cost is low; the operation is simple; the proliferation speed is high; the breeding coefficient is high; good economic benefits and ecological benefits are realized; the monstera deliciosa tissue culture method is applicable to scale production.

Description

【Technical field】 [0001] The invention relates to the technical field of plant cultivation and reproduction, in particular to a method for culturing monstera tissue. 【Background technique】 [0002] Monstera, (scientific name: Monstera deliciosa). Also known as "Penglai banana", "wire grass", evergreen vines. The stem is strong; the young leaves are heart-shaped without holes, and become broadly ovate and pinnately parted when they grow up; there are oval perforations between the veins, and the leaves are long-stalked and dark green. Spatha inflorescence, spathe boat-shaped, blooms in November, pale yellow. There are brown aerial roots on the stem, which are shaped like electric wires, so it is named "wire grass"; the leaves are oval, and there are many oblong holes and deep cracks scattered between the feathery veins in the shape of tortoise shells. The tortoise shell pattern, the stem is knotted like a bamboo stem, hence the name "Monstera". Spadix, the whole flower sha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 覃伟杰
Owner 贵港沃斯顿科技有限公司
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