A kind of method of monstera tissue culture
A technology of tissue culture and monstera, which is applied in the field of plant cultivation and reproduction, can solve the problems of wasting branches, cutting propagation is difficult to meet large-scale production, and root system is underdeveloped, so as to achieve convenient material acquisition, good economic and ecological benefits, and low cost Effect
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Embodiment 1
[0029] A kind of method of monstera tissue culture provided by the present embodiment comprises the following steps:
[0030] (1) Pretreatment of explants: cut the stem top or axillary buds of Monstera as explants, cut the explants into 4cm strips, then soak the explants for 3s with 70% alcohol, and then Soak in 1% sodium hypochlorite solution for 15 minutes, oscillate continuously during the soaking process, rinse the explants with sterile water 4 times after soaking, then place them on a sterile inoculation tray to dry the surface moisture, cut off the leaves and old Incision, cut into 2 cm stems with 2 buds, rinse the buds with tap water for 30 minutes, and dry the buds with filter paper for later use;
[0031] (2) Cultivation of medium:
[0032] (1) Induction culture: Inoculate the budded stem section obtained in the above steps on the induction medium, and cultivate it under dark conditions for 5 days, and then carry out induction culture alternately according to the lig...
Embodiment 2
[0038] A kind of method of monstera tissue culture provided by the present embodiment comprises the following steps:
[0039] (1) Pretreatment of explants: cut the stem top or axillary buds of Monstera as explants, cut the explants into 5cm strips, then soak the explants for 4s with 70% alcohol, and then Soak in 1.2% sodium hypochlorite solution for 22 minutes, oscillate continuously during the soaking process, rinse the explants with sterile water 5 times after soaking, then place them on a sterile inoculation tray to dry the surface moisture, cut off the leaves and old Incision, cut into 2.2cm stem section with 2 buds, rinse the bud stem section under tap water for 30 minutes, dry the bud stem section with filter paper for later use;
[0040] (2) Cultivation of medium:
[0041] (1) Induction culture: inoculate the budded stem segment obtained in the above steps on the induction medium, and cultivate it under dark conditions for 7 days, and then carry out induction culture a...
Embodiment 3
[0047] A kind of method of monstera tissue culture provided by the present embodiment comprises the following steps:
[0048] (1) Pretreatment of explants: cut the stem top or axillary buds of Monstera as explants, cut the explants into 6cm strips, then soak the explants for 5s with 70% alcohol, and then Soak in 1.5% sodium hypochlorite solution for 30 minutes, oscillate continuously during the soaking process, rinse the explants with sterile water 6 times after soaking, then place them on a sterile inoculation tray to dry the surface moisture, cut off the leaves and old Incision, cut into 2.5cm stems with 3 buds, rinse the stems with buds under tap water for 30 minutes, dry the stems with buds with filter paper for later use;
[0049] (2) Cultivation of medium:
[0050] (1) Induction culture: inoculate the budded stem section obtained in the above steps on the induction medium, and cultivate it under dark conditions for 10 days, then carry out induction culture alternately a...
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