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Method of quickly breeding Chinese fir by tissue culture

A tissue culture and Chinese fir technology, applied in the field of plant cultivation and reproduction, can solve the problems of restricting the popularization and application of high-quality varieties, insufficient supply of fine Chinese fir seedlings, restricting the development of Chinese fir plantations, etc., and achieves good economic and ecological benefits, low cost, The effect of fast propagation

Inactive Publication Date: 2017-07-07
河池乐康生态农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Insufficient supply of Chinese fir seedlings seriously restricts the development of Chinese fir plantations
[0005] Chinese fir is usually propagated by seeds and cuttings. As a traditional method of raising Chinese fir seedlings, there are many defects, such as uneven seedlings, high seedling cost, obvious differentiation, and great variation in offspring, etc., which seriously restricts the popularization and application of many high-quality varieties.

Method used

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  • Method of quickly breeding Chinese fir by tissue culture
  • Method of quickly breeding Chinese fir by tissue culture

Examples

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Effect test

Embodiment 1

[0029] The present embodiment provides a kind of method for rapidly breeding Chinese fir through tissue culture, specifically comprising the following steps:

[0030] (1) preparation of explant: select single plant, excellent plant Chinese fir as female parent, female parent is carried out root circumcision process, after root grows tender bud, pick its tender shoot branch and carry out tissue culture as explant, The shoots are semi-lignified branches that germinate from April to August, and the shoots are picked after at least 3 days in fine weather;

[0031] (2) pretreatment of explant: clipping is explant through the new long bud branch of circumcision root, explant is cut into the branch of 5cm, soaks 12s with 75% alcohol, soaks 30min with onion juice then, During the soaking period, the glass rods were used to stir continuously. After the soaking, the explants were rinsed 5 times with sterile water, and then the explants were placed on the ultra-clean workbench until the ...

Embodiment 2

[0039] The present embodiment provides a kind of method for rapidly breeding Chinese fir through tissue culture, specifically comprising the following steps:

[0040] (1) preparation of explant: select single plant, excellent plant Chinese fir as female parent, female parent is carried out root circumcision process, after root grows tender bud, pick its tender shoot branch and carry out tissue culture as explant, The shoots are semi-lignified branches that germinate from April to August, and the shoots are picked after at least 3 days in fine weather;

[0041] (2) pretreatment of explant: clipping is explant through the new long bud branch of circumcision root, explant is cut into the branch of 6cm, soaks 15s with 75% alcohol, soaks 40min with onion juice then, During the soaking period, the glass rods were used to stir continuously. After the soaking, the explants were rinsed 6 times with sterile water, and then the explants were placed on the ultra-clean workbench until the ...

Embodiment 3

[0049] The present embodiment provides a kind of method for rapidly breeding Chinese fir through tissue culture, specifically comprising the following steps:

[0050] (1) preparation of explant: select single plant, excellent plant Chinese fir as female parent, female parent is carried out root circumcision process, after root grows tender bud, pick its tender shoot branch and carry out tissue culture as explant, The shoots are semi-lignified branches that germinate from April to August, and the shoots are picked after at least 4 days in fine weather;

[0051] (2) pretreatment of explant: clipping is explant through the new long bud branch of circumcision root, explant is cut into the branch of 7cm, soaks 16s with 75% alcohol, soaks 50min with onion juice then, During the soaking period, the glass rods were used to stir continuously. After soaking, the explants were washed 7 times with sterile water, and then the explants were placed on the ultra-clean workbench until the surf...

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Abstract

The present invention provides a kind of method of tissue culture rapid breeding Chinese fir, relates to the technical field of plant breeding and propagation, the method of tissue culture rapid breeding Chinese fir of the present invention comprises the following steps: (1) the preparation of explant, (2) the preparation of explant Pretreatment, (3) cultivation of culture medium, (4) transplanting of rooted seedlings; the method for rapid breeding of Chinese fir by tissue culture of the present invention induces the callus of Chinese fir on MS medium containing different hormone ratios, through induction culture culture medium, proliferation medium and rooting medium, each medium provides the factors needed for explants during rooting and strong seedlings, can provide good conditions for rooting, promote healthy and strong seedlings, and obtain regenerated seedlings of Chinese fir. The invention has the advantages of convenient material collection, low cost, simple operation, fast multiplication speed, high reproduction coefficient, good economic and ecological benefits, and is suitable for large-scale production.

Description

[0001] 【Technical field】 [0002] The invention relates to the technical field of plant cultivation and reproduction, in particular to a method for rapid propagation of Chinese fir by tissue culture. [0003] 【Background technique】 [0004] The tree is an evergreen tree belonging to the fir family, also known as thorny fir. Chinese fir is one of the unique evergreen coniferous tree species in my country. Chinese fir grows fast and can become useful in 8-15 years. Chinese fir is widely distributed throughout the entire subtropical region of my country. The cultivation area reaches 16 provinces (autonomous regions), reaching Zhejiang and Fujian in the east, Yunnan in the west, the western edge of the Sichuan Basin and the Anning River area, central Guangdong and central and southern Guangxi in the south, and central and southern Guangxi in the south. To the southern foot of the Qinling Mountains. Chinese fir grows fast, has straight and round stems, and is famous for its good mat...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G17/00A01G7/06
CPCA01H4/008A01G7/06A01G17/005A01H4/001
Inventor 罗墩陈婉霞唐庆乐
Owner 河池乐康生态农业科技有限公司
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