Gluconacetobacter oboediens strain and method for breeding and producing bacteria cellulose thereof

A technology of bacterial cellulose and xylogluconic acid, applied in the field of microorganisms, can solve problems such as not having much value, and achieve the effects of reducing production costs, preventing the contamination of miscellaneous bacteria, and improving yield

Active Publication Date: 2009-12-23
HAINAN YEGUO FOODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the "low temperature" mentioned in the literature refers to cultivation at 25°C, which has little value in actual production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Screen the gluconacetoacetobacter xylinum with thick cellulose film and strong vigor at room temperature, insert it into the sterilized medium for static culture, select the temperature of the incubator at 30°C as the initial temperature, and cultivate it for 3 days as the second temperature. For the first-generation strains, adjust the temperature of the incubator to 25°C, select the cellulose-producing bacterial solution with a thicker cellulose film in the first-generation strains as the seed liquid, insert it into the sterilized medium for static cultivation, and cultivate it for 3 days as the seed liquid. The second-generation strains, and so on, reduce the culture temperature by 5°C for each generation of transfer, and at the same time screen the cellulose-film-thick bacterial liquid of the previous generation of strains each time as the seed liquid for the next-generation culture. After 5 generations of screening, A strain of the genus Gluconacetobacter xylinum th...

Embodiment 2

[0033]Screen the gluconacetoacetobacter xylinum with thick cellulose film and strong vigor at room temperature, insert it into the sterilized medium for static culture, select the temperature of the incubator at 28°C as the initial temperature, and cultivate it for 2 days as the second. For the first-generation strains, adjust the temperature of the incubator to 25°C, select the cellulose-producing bacterial solution with a thicker cellulose film in the first-generation strains as the seed liquid, insert it into the sterilized medium for static cultivation, and cultivate it for 3 days as the seed liquid. The second generation of strains, and so on, the culture temperature is lowered by 3°C for each transfer generation, and at the same time, the bacterial solution that produces cellulose film thickness in the previous generation of strains is screened each time as the seed liquid for the next generation of culture. After 5 generations of screening, A strain of the genus Gluconac...

Embodiment 3

[0040] Screen the gluconacetoacetobacter xylinum with thick cellulose film and strong vigor at room temperature, insert it into the sterilized medium for static culture, select the temperature of the incubator at 30°C as the initial temperature, and cultivate it for 3 days as the second temperature. For the first-generation strains, adjust the temperature of the incubator to 25°C, select the cellulose-producing bacterial solution with a thicker cellulose film in the first-generation strains as the seed liquid, insert it into the sterilized medium for static cultivation, and cultivate it for 3 days as the seed liquid. The second-generation strains, and so on, reduce the culture temperature by 5°C for each generation of transfer, and at the same time select the cellulose-film-thick bacterial solution from the previous generation of strains each time as the seed liquid for the next-generation culture. After three generations of screening, A strain of the genus Gluconacetobacter xy...

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Abstract

The invention provides a gluconacetobacter xylinums strain and a method for breeding and producing bacteria cellulose thereof. The strain is collected in a common microorganism center in the China type microorganism culture collection committee on July 12th 2004, and the collection registering number is CGMCCNO.1186, the name is gluconacetobacter xylinum 323, and the classification name is gluconacetobacter xylinum. The method for breading and producing bacteria cellulose comprises the following steps: sieving gluconacetobacter xylinum producing thick cellulose membrane with strong activity under room temperature; cooling and culturing then sieving; cooling and culturing, and sieving the gluconacetobacter xylinums strain which can produce the bacteria cellulose at 10-20 DEG C. the method for producing the bacteria cellulose comprises the following steps: strain expanding culture, culture medium preparation, culture medium panning and inoculation fermentation. The strain can break through the limit of the temperature, lowers the production cost, and effectively prevents living contaminants during the production, thereby improving the yield.

Description

technical field [0001] The invention relates to a microorganism, in particular to a low-temperature-resistant strain of the genus Gluconacetobacter xylinum and its selection and application method. Background technique [0002] The cellulose produced by the fermentation of microorganisms (mainly Acetobacter) is generally called bacterial cellulose or microbial cellulose. This cellulose has many characteristics different from plant fibers such as high purity, strong water absorption, high crystallinity, and high polymerization degree. The structural characteristics of the element, its fiber bundle diameter is less than 100 nanometers, it is a kind of natural nano-biological material, and it is one of the hot spots in the research and development of biological materials in recent years. The industry has broad application prospects. At present, this kind of bacterial cellulose is widely used in food. The main method is to use coconut as the medium, and produce milky white or t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/02
Inventor 钟春燕
Owner HAINAN YEGUO FOODS
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