Microorganism modifier and preparation method and application thereof

An improvement agent and microbial technology, applied in the field of microorganisms, can solve the problems of high logistics and transportation costs, discounts for beneficial effects, and dilution concentrations that cannot exceed 100 times

Pending Publication Date: 2020-10-23
广东理想生物科学研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the microbial additives currently on the market can only improve the digestive function of poultry intestines, enhance immunity, and play a preventive and health-care role, but have no therapeutic effect on sick poultry. For example, patent 201610110688.8 discloses a compound bacterium that enhances animal immunity The compound bacterial agent can regulate the balance of intestinal

Method used

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  • Microorganism modifier and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of Microbial Improving Bacteria Agent

[0032] (1) Solid slope culture: Bacillus subtilis, Saccharomyces cerevisiae, Clostridium butyricum, Lactobacillus plantarum, Pediococcus pentosaceae, Enterococcus faecalis, Acetobacter, Bifidobacterium, Lactobacillus, Lactobacillus acidophilus, cheese milk Bacillus, photosynthetic bacteria, actinomycetes, Clostridium butyricum, and Bacillus coagulans were respectively inoculated and cultured on a solid slant medium to fully activate the bacteria. The solid slant medium included tryptone 15g, beef extract 8g, and glucose 20g , sodium chloride 0.5g, distilled water to make up 1000mL, agar 10g, pH7.4, sterilization conditions: 121℃, 30min;

[0033] (2) The activated Bacillus phlox, Saccharomyces cerevisiae, Clostridium butyricum, Lactobacillus plantarum, Pediococcus pentosaceae, Enterococcus faecalis, Acetobacter, Bifidobacterium, Lactobacillus, Lactobacillus acidophilus, Lactobacillus casei, photosynthetic bac...

Embodiment 2

[0036] Embodiment 2 Microbial improvement bacteria agent

[0037] The microbial improvement agent is in the form of bacterial liquid, including: the number of live bacteria of Bacillus subtilis is 1×10 9 —8x10 10 CFU / mL, the viable count of Saccharomyces cerevisiae is 3x10 4 —8x10 6 CFU / mL, Clostridium butyricum viable count is 2x10 6 —9x10 8 CFU / mL, the viable count of Lactobacillus plantarum is 1x10 4 —5x10 6 CFU / mL, the viable count of Pediococcus pentosaceae is 2x10 6 —5x10 8 CFU / mL, Enterococcus faecalis viable count is 2x10 6 —1x10 8 CFU / mL, the viable count of Acetobacter is 3x10 6 —7x10 8 CFU / mL, Bifidobacterium viable count is 1x10 6 —5x10 9 CFU / mL, Lactobacillus viable count is 2x10 6 —6x10 8 CFU / mL, Lactobacillus acidophilus viable count is 3x10 8 —8x10 10 CFU / mL, the viable count of Lactobacillus casei is 1x10 4 —6x10 6 CFU / mL, the number of viable photosynthetic bacteria is 1x10 4 —9x10 6 CFU / mL, the number of viable actinomycetes is 2x10 5 —...

Embodiment 3

[0048] Example 3 Application of Microbial Improving Bacteria Agent in Poultry or Livestock Raising

[0049] This example takes pig as an example, and other poultry or domestic animals such as chickens, ducks, geese, pigeons, partridges, quails, cattle, sheep, etc. are used in the same way as in this embodiment.

[0050] 1. Application of microbial improvement agent in healthy pigs

[0051] Select 1100 weaned piglets with similar body size and state, and randomly divide them into 11 groups of 100 piglets, of which ten groups are set as drinking probiotics groups: the microbial improvement liquid bacterial agent 1-10 bacteria liquid ratio prepared in Example 2 The ratio of water 1:600 ​​times is diluted and the pigs are given daily drinking water. The other group is the control group, without adding the microbe-improved liquid inoculum, and drinking the same amount of water instead. After 42 days of continuous feeding, the average values ​​of each index in each group were calc...

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Abstract

The invention discloses a microorganism modifier and a preparation method and application thereof. The microorganism modifier is prepared from: Bacillus subtilis, Saccharomyces cerevisiae, Clostridiumbutyricum, Lactobacillus plantarum, Pediococcus pentosaceus, Enterococcus faecalis, Acetobacter, Bifidobacterium, Lactobacillus, Lactobacillus acidophilus, Lactobacillus casei, Photosynthetic bacteria, Actinomycetes, Clostridium butyricum and Bacillus coagulans. According to the microorganism modifier, various bacterial groups are cooperated and interacted to produce good effects, and the microorganism modifier can be widely used in breeding of various poultry or livestock. Meanwhile, the microorganism modifier can significantly enhance the immunity of poultry or livestock, promote nutrient absorption, increase feed utilization rate, and improve meat quality; and the microorganism modifier has a good therapeutic effect on poultry or livestock that have common diseases or even serious diseases, and the economic benefits of farmers can be significantly improved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms. More specifically, it relates to a microbial modifier and its preparation method and application. Background technique [0002] In the livestock and poultry breeding industry, viral and bacterial diseases are common and frequently-occurring diseases. In order to improve the health immunity of livestock and poultry, the usual measures are to add some antibiotics, growth-stimulating hormones, chemical drugs, etc. to the livestock and poultry feed. conventional feed additives. With the improvement of human living standards, food safety issues have attracted much attention. The use of antibiotics, growth-stimulating hormones, chemical drugs and other additives in feed will not only affect the healthy growth of animals and the quality of meat products, but also cause certain safety hazards to food safety, which greatly threatens human health. [0003] Microbial additives are non-toxic, side-...

Claims

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Application Information

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IPC IPC(8): A23K10/18A01K67/02C12N1/20C12N1/16C12R1/125C12R1/865C12R1/145C12R1/01C12R1/02C12R1/225C12R1/23C12R1/245C12R1/04C12R1/07
CPCA23K10/18A01K67/02C12N1/20C12N1/16
Inventor 郭旭生黄志清
Owner 广东理想生物科学研究有限公司
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