Citrus fruit vinegar prepared by composite strain mixed fermentation and preparation method thereof

A technology of mixed fermentation and compound strains, applied in microorganism-based methods, biochemical equipment and methods, vinegar preparation, etc., can solve the problems of limited fermentation time, easy contamination by miscellaneous bacteria, poor flavor and taste, etc. Soft taste effect

Active Publication Date: 2015-06-10
LISHUI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the liquid fermentation method, the solid-state fermentation method has the advantages of good flavor and good taste, but there are disadvantages such as long fermentation period, high labor intensity, difficult to control the fermentation process, and easy to be contaminated by bacteria.
The advantage of the liquid fermentation method is that the fermentation cycle is short, the labor intensity is small, the fermentation process i...

Method used

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  • Citrus fruit vinegar prepared by composite strain mixed fermentation and preparation method thereof
  • Citrus fruit vinegar prepared by composite strain mixed fermentation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the preparation technology one of citrus fruit vinegar

[0024] one, Material preparation process

[0025] (1) Preparation of compound yeast seed liquid: the volume of yeast activation medium is 150mL / 250mL, and two bait yeasts 31906, Saccharomyces cerevisiae 1306, Saccharomyces cerevisiae 1425, and Hansenula anomaly var. Transfer to the culture solution respectively, place on a shaker at 28°C, and incubate at 180r / min for 16 hours to obtain a first-class culture solution, adjust the cell concentration to 10 7 Individuals / mL; four kinds of primary culture medium were inserted into the malt medium according to the following volume percentages: 4% Hansenula anomaly var. , and then placed in a shaker at 28°C, 180r / min and cultivated for 16h, so that the number of bacteria reached 10 7 -10 8 Each / mL is the compound yeast seed solution.

[0026] (2) Preparation of compound acetic acid bacteria seed solution: the volume of acetic acid bacteria activation...

Embodiment 2

[0036] Embodiment 2: the preparation process two of citrus fruit vinegar

[0037] one, Material preparation process

[0038] (1) Preparation of compound yeast seed liquid: the volume of yeast activation medium is 150mL / 250mL, and two bait yeasts 31906, Saccharomyces cerevisiae 1306, Saccharomyces cerevisiae 1425, and Hansenula anomaly var. Transfer to the culture solution respectively, place on a shaker at 28°C, and incubate at 180r / min for 16 hours to obtain a first-class culture solution, adjust the cell concentration to 10 7 Individuals / mL; four kinds of primary culture medium were inserted into the malt medium according to the following volume percentages: 4% Hansenula anomaly var. , and then placed in a shaker at 28°C, 180r / min and cultivated for 16h, so that the number of bacteria reached 10 7 -10 8 Each / mL is the compound yeast seed solution.

[0039] (2) Preparation of compound acetic acid bacteria seed solution: the volume of acetic acid bacteria activation me...

Embodiment 3

[0049] Example 3: Isolation and Identification of Apple Acetobacter ZY.C4

[0050] (1) Bacterial source preparation Commercially purchased citrus without rot, washed, beaten, and subjected to natural alcoholic fermentation and natural acetic acid fermentation. Measure its sugar content with a sugar meter every day, when the sugar content drops to 5 ° BX, and does not change for a week, start to filter. Filter with a double layer of boiled 80 mesh gauze, and store the filtrate in a stainless steel pot after filtering, that is, citrus wine. Cover the surface of the pot with gauze, and carry out natural fermentation at 32°C to obtain naturally fermented vinegar liquid. After 5 days, the bacteria source was taken from the vinegar solution for screening and isolation.

[0051] (2) Separation and screening process

[0052] Bacteria source—proliferation culture—dilution plate separation—preliminary screening—incline culture—liquid Erlenmeyer flask culture—confirmation of primary...

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Abstract

The invention relates to a citrus fruit vinegar prepared by composite strain mixed fermentation, belonging to the technical field of fruit vinegar brewage. The preparation method is characterized by comprising the following steps: 1) preparing a composite microzyme strain seed solution; 2) preparing a composite acetobacter strain seed solution; 3) preparing a glutinous rice saccharified solution; 4) preparing a citrus juice; 5) preparing a fermentation liquid; 6) carrying out alcohol fermentation; 7) carrying out acetic fermentation; and 8) aging at room temperature for 2-3 months, filtering with diatomite, and clarifying to obtain the citrus fruit vinegar finished product. By using the citrus and glutinous rice as the raw materials and adopting the fruit/grain co-brewage and composite strain mixed fermentation, the prepared citrus fruit vinegar has the advantages of abundant nutrition, favorable mouthfeel, pleasant fruital aroma and no bitterness.

Description

technical field [0001] The invention belongs to the technical field of fruit vinegar brewing, and in particular relates to citrus fruit vinegar prepared by mixed fermentation of compound strains and a preparation method thereof. Background technique [0002] Citrus fruit vinegar is made from fresh and ripe citrus fruits, which are produced by peeling, juicing, separation, debittering, alcoholic fermentation, acetic acid fermentation, aging, and sterilization. It is rich in organic acids and vitamins, have health effects such as relieving heat and thirst, eliminating fatigue, beautifying and beautifying, increasing appetite, relieving alcohol and protecting the liver, antiseptic and sterilization, etc. The curative and preventive effect, is a new generation of fermented food. Safe and efficient production of citrus fruit vinegar with strong fruity aroma and unique flavor is the key to the healthy development of its industrialization. Fruit vinegar needs to have unique flav...

Claims

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Application Information

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IPC IPC(8): C12J1/04C12R1/865C12R1/78C12R1/02
CPCC12J1/04
Inventor 刘青娥曹鹏飞叶选怡
Owner LISHUI UNIV
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