Citrus fruit vinegar prepared by composite strain mixed fermentation and preparation method thereof
A technology of mixed fermentation and compound strains, applied in microorganism-based methods, biochemical equipment and methods, vinegar preparation, etc., can solve the problems of limited fermentation time, easy contamination by miscellaneous bacteria, poor flavor and taste, etc. Soft taste effect
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Embodiment 1
[0023] Embodiment 1: the preparation technology one of citrus fruit vinegar
[0024] one, Material preparation process
[0025] (1) Preparation of compound yeast seed liquid: the volume of yeast activation medium is 150mL / 250mL, and two bait yeasts 31906, Saccharomyces cerevisiae 1306, Saccharomyces cerevisiae 1425, and Hansenula anomaly var. Transfer to the culture solution respectively, place on a shaker at 28°C, and incubate at 180r / min for 16 hours to obtain a first-class culture solution, adjust the cell concentration to 10 7 Individuals / mL; four kinds of primary culture medium were inserted into the malt medium according to the following volume percentages: 4% Hansenula anomaly var. , and then placed in a shaker at 28°C, 180r / min and cultivated for 16h, so that the number of bacteria reached 10 7 -10 8 Each / mL is the compound yeast seed solution.
[0026] (2) Preparation of compound acetic acid bacteria seed solution: the volume of acetic acid bacteria activation...
Embodiment 2
[0036] Embodiment 2: the preparation process two of citrus fruit vinegar
[0037] one, Material preparation process
[0038] (1) Preparation of compound yeast seed liquid: the volume of yeast activation medium is 150mL / 250mL, and two bait yeasts 31906, Saccharomyces cerevisiae 1306, Saccharomyces cerevisiae 1425, and Hansenula anomaly var. Transfer to the culture solution respectively, place on a shaker at 28°C, and incubate at 180r / min for 16 hours to obtain a first-class culture solution, adjust the cell concentration to 10 7 Individuals / mL; four kinds of primary culture medium were inserted into the malt medium according to the following volume percentages: 4% Hansenula anomaly var. , and then placed in a shaker at 28°C, 180r / min and cultivated for 16h, so that the number of bacteria reached 10 7 -10 8 Each / mL is the compound yeast seed solution.
[0039] (2) Preparation of compound acetic acid bacteria seed solution: the volume of acetic acid bacteria activation me...
Embodiment 3
[0049] Example 3: Isolation and Identification of Apple Acetobacter ZY.C4
[0050] (1) Bacterial source preparation Commercially purchased citrus without rot, washed, beaten, and subjected to natural alcoholic fermentation and natural acetic acid fermentation. Measure its sugar content with a sugar meter every day, when the sugar content drops to 5 ° BX, and does not change for a week, start to filter. Filter with a double layer of boiled 80 mesh gauze, and store the filtrate in a stainless steel pot after filtering, that is, citrus wine. Cover the surface of the pot with gauze, and carry out natural fermentation at 32°C to obtain naturally fermented vinegar liquid. After 5 days, the bacteria source was taken from the vinegar solution for screening and isolation.
[0051] (2) Separation and screening process
[0052] Bacteria source—proliferation culture—dilution plate separation—preliminary screening—incline culture—liquid Erlenmeyer flask culture—confirmation of primary...
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