Method for preparing antioxidative peptide through fermentation of cordyceps militaris nanoparticles

A nanoparticle and antioxidant peptide technology, applied in the field of microbial fermentation, can solve the problem that Cordyceps militaris has not been reported in literature and the like, and achieve the effects of improving human immunity, simplifying the preparation method and reducing the production cost.

Active Publication Date: 2015-07-22
JILIN SERICULTURE SCI RES INST
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  • Abstract
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Problems solved by technology

At present, the research on the deep processing of Cordyceps militaris by combining nanotechnology with microbial fermentation has not been reported in the literature

Method used

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  • Method for preparing antioxidative peptide through fermentation of cordyceps militaris nanoparticles
  • Method for preparing antioxidative peptide through fermentation of cordyceps militaris nanoparticles
  • Method for preparing antioxidative peptide through fermentation of cordyceps militaris nanoparticles

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Embodiment Construction

[0022] A method for preparing antioxidant peptides by fermenting Cordyceps militaris nanoparticles of the present invention comprises the following steps:

[0023] (1) drying Cordyceps militaris at a temperature of 60-80°C, coarsely pulverizing through a 40-mesh sieve, and then nano-crushing for 3-5 hours to obtain Cordyceps militaris nanoparticles;

[0024] (2) According to the 1:20 ratio of Cordyceps militaris nanoparticles to distilled water, 5g of Cordyceps militaris nanoparticles was added to 100mL of distilled water, shaken well, and the natural pH value was sterilized in an autoclave at 121°C for 20-30min to obtain seed medium;

[0025] (3) Add 100ml of distilled water into a beaker, add 0.3g of beef extract, 1.0g of peptone, 0.5g of sodium chloride, pH at 6-7, sterilize by high-pressure steam at 121°C for 20-30min, and cool to obtain common beef extract peptone Culture medium, inoculate common beef extract peptone culture medium with Bacillus licheniformis strain slan...

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Abstract

A method for preparing antioxidative peptide through fermentation of cordyceps militaris nanoparticles is characterized by comprising the steps: drying cordyceps militaris at the temperature of 60-80 DEG C, carrying out coarse crushing, passing through a 40-mesh sieve, then carrying out nano crushing for 3-5 h, and thus obtaining cordyceps militaris nanoparticles; adding the cordyceps militaris nanoparticles to distilled water, shaking evenly, adjusting to a natural pH value, sterilizing for 20-30 min in a high-pressure sterilization pot at the temperature of 121 DEG C, and thus obtaining a seed culture medium; putting a beef extract, peptone and sodium chloride in distilled water, allowing the pH to be 6-7, carrying out high-pressure steam sterilization for 20-30 min at the temperature of 121 DEG C, cooling to obtain an ordinary beef extract-peptone culture medium, inoculating the ordinary beef extract-peptone culture medium with a slant strain of a bacillus licheniformis strain, at the temperature of 30-40 DEG C, culturing for 1-2 d, to obtain inoculated and activated bacillus licheniformis, inoculating the seed culture medium with the activated bacillus licheniformis, and thus obtaining the antioxidative peptide product. The method is high in efficiency, low in cost, short in production cycle, and suitable for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and relates to a method for preparing antioxidant peptides by fermenting Cordyceps militaris nanoparticles. Background technique [0002] Modern research has found that almost all diseases are related to free radicals. Free radicals have a high degree of activity and a strong ability to oxidize. At present, the development and utilization of natural antioxidants have been widely carried out. As far as our country is concerned, there are disadvantages of single type, low productivity and poor product stability. With its unique advantages of less dosage and strong activity, bioactive peptides have attracted more and more attention, and its industrial production has become a major trend in the development of the current food and pharmaceutical industries. It has been reported that the peptides with antioxidant activity from enzymatic hydrolysis or extraction from food protein source...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C12R1/10
Inventor 李金志李森杨淑芳韩建华刘隽彦朱兴友孙继红薛强靳向东
Owner JILIN SERICULTURE SCI RES INST
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