Solid phase polypeptide synthesis preparation method for salcatonin

Abstract

The invention discloses a new salmon calcimar preparing method of Fmoc-strategy solid-phase polypeptide, which comprises the following steps: a. adopting Fmoc-Rink Amide MBHA or Rink Amide AM resin to connect the 32-piptide resin protected by kinds of amino acid after eluting Fmoc-protection sequently; b. eluting Fmoc-protective group sequently; c. removing side-chain protective group and cutting peptide synchronously to obtain reduced crude product; d. making crude product to do oxidation reaction through (7.5-10.0 pH) air; proceeding inverse-phase HPLC separation and purifying to produce fine salmon calcimar. The invention possesses scale producing capacity, which improves the quality, obtaining rate, and total obtaining rate.

Description

technical field

[0001] The invention relates to a preparation method of salmon calcitonin, in particular to a preparation method of solid-phase polypeptide synthesis of salmon calcitonin. Background technique

[0002] Salmon calcitonin, English name: Calcitonin (Salmon), trade name: Miacaicic, chemical structural formula:

[0003]

[0004] Molecular formula and molecular weight: C 145 h 240 N 44 o 48 S 2 3431.9

[0005] Salmon calcitonin is clinically used in the treatment of osteoporosis. Natural calcitonin (CT) is a biologically active polypeptide secreted by parathyroid cells of mammals or hind parotids of non-mammalian vertebrates. It is the main regulator of calcium metabolism in organisms. It comes from humans, pigs CT extracts or synthetic products of , salmon, eel, etc. have been developed into medicines, among which salmon calcitonin (sCT) has a strong and long-lasting clinical effect, which is 32 times more active than human calcitonin (hCT) and has a lo...

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