Extraction method of tuna polypeptide

An extraction method and technology of tuna, which is applied in the field of extraction of tuna peptides, can solve the problems of high peptide yield and fishy smell of tuna peptides, and achieve the effects of high peptide yield, easy absorption by human body, and energy saving

Active Publication Date: 2015-12-16
山东思科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the fishy smell of tuna polypeptide extracted by the existing method, and provide a method for extracting tuna polypeptide. The tuna freeze-dried powder prepared by the present invention not only has no fishy smell but also has a high yield of polypeptide

Method used

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  • Extraction method of tuna polypeptide
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Examples

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Embodiment 1

[0028] A method for extracting tuna polypeptides, comprising the following steps:

[0029] (1) Wash the fresh tuna leftovers and twist them into minced tuna;

[0030] (2) Mix the minced tuna meat with the NaCl solution with a concentration of 0.4mol / L at a mass ratio of 1:8, and use a magnetic constant temperature stirrer to extract the mixed solution at 10°C and a stirring speed of 500r / min for 1 hour;

[0031] (3) After the leaching is completed, filter the leaching solution to obtain the feed liquid, use 0.01mol / L hydrochloric acid solution to adjust the pH of the feed liquid to 5.0, and then add modified bentonite with a mass ratio of 1:10 to the feed liquid , react for 45min;

[0032] (4) After the reaction is completed, use 0.01mol / L NaOH solution to adjust the pH of the feed solution to 7.0, centrifuge the feed solution at 8000r / min for 20min, and take the supernatant;

[0033] (5) Add neutral protease and compound protease accounting for 4% of the mass of the superna...

Embodiment 2

[0040] A preparation method of tuna freeze-dried powder, comprising the following steps:

[0041] (1) Clean the fresh tuna after removing the viscera, and twist it into minced tuna;

[0042] (2) Mix the minced tuna meat and the NaCl solution with a concentration of 0.45mol / L at a mass ratio of 1:9, and use a magnetic stirrer to extract the mixed solution at 12°C and a stirring speed of 600r / min for 1.5h;

[0043] (3) After the leaching is completed, filter the leaching solution to obtain the feed liquid, use 0.01mol / L hydrochloric acid solution to adjust the pH of the feed liquid to 5.2, and then add modified bentonite with a mass ratio of 1:11 to the feed liquid , react for 50min;

[0044] (4) After the reaction is completed, use 0.01mol / L NaOH solution to adjust the pH of the feed solution to neutrality, then centrifuge the feed solution at 9000r / min for 25min, and take the supernatant;

[0045] (5) Add neutral protease and compound protease accounting for 5% of the mass o...

Embodiment 3

[0052] A preparation method of tuna freeze-dried powder, is characterized in that, comprises the following steps:

[0053] (1) Clean the fresh tuna after removing the viscera, and twist it into minced tuna;

[0054] (2) Mix the minced tuna meat and the NaCl solution with a concentration of 0.5mol / L at a mass ratio of 1:10, and use a magnetic stirrer to extract the mixed solution at 15°C and a stirring speed of 700r / min for 2 hours;

[0055] (3) After the leaching is completed, filter the leaching solution to obtain the feed liquid, use 0.01mol / L hydrochloric acid solution to adjust the pH of the feed liquid to 5.5, and then add modified bentonite with a mass ratio of 1:12 to the feed liquid , react for 60min;

[0056] (4) After the reaction is completed, use 0.01mol / L NaOH solution to adjust the pH of the feed solution to neutrality, then centrifuge the feed solution at 10000r / min for 30min, and take the supernatant;

[0057] (5) Add neutral protease and compound protease ac...

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Abstract

The invention discloses an extraction method of tuna polypeptide and aims to mainly solve the problem that tuna polypeptide extracted by an existing method has fishy smell. The method includes: extracting protein in tuna processing leftovers via a thin salt solution, removing the fishy smell from the extracted tuna protein via modified bentonite, adjusting pH of tuna protein liquid before fishy smell removal according to the principle isoelectric precipitation of protein, precipitating the extracted tuna protein, thereby avoiding adsorption of the modified bentonite for the protein, enzymatically hydrolyzing the protein via neutral protease and compound protease to obtain the tuna polypeptide. The tuna polypeptide extracted by the method has no fishy smell and is high in yield.

Description

technical field [0001] The invention relates to the field of aquatic product processing, in particular to a method for extracting tuna polypeptide. Background technique [0002] Tuna is a general term for several genera of fish with a breastplate in the bony fish class Perciformes, also known as tuna and tuna, and is a highly migratory fish that lives in the middle and upper waters of the ocean , mainly in the tropical, subtropical and temperate waters of the Pacific Ocean, Atlantic Ocean, and Indian Ocean. There are 5 genera and 17 species of common tuna. Mainly include albacore tuna, Atlantic tuna, yellowfin tuna, bigeye tuna, bluefin tuna, skipjack tuna, etc. Tuna meat is fresh and tender, with high protein content, biological value as high as 90, and excellent amino acid combination; it is rich in DHA, EPA and other biologically active highly polyunsaturated fatty acids, which can promote brain development and effectively prevent cardiovascular and cerebrovascular dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/30C07K1/22
Inventor 杨会成
Owner 山东思科生物科技有限公司
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