Preparing process for synthesizing oxytocin from solid-phase polypeptide

Abstract

The invention discloses a method for preparing oxytocin through solid phase polypeptide synthesis, comprising following steps: taking Rink Amide resin (comprising Rink Amide MBHA resin, Rink Amide Am resin) as raw material, taking amino acid protected by Fmoc, TBTU or HBTU / HOBt as condensing agent, making up amino acid in sequence; adding peptide cutting agent for peptide cutting, adding for precipitation and getting reduced coarse product; adding basic matter, feeding air for oxidation or oxiding with H2O2 with pH being 7.5- 10.0, getting oxidized coarse product; separating and purifying by using C18 or C 8 column and getting final product. The method is characterized by low production cost, simple process, little pollution, high production rate and convenience for industrial production.

Description

technical field

[0001] The invention relates to a preparation method of oxytocin, in particular to a preparation method of solid-phase polypeptide synthesis of oxytocin. Background technique

[0002] Oxytocin, also known as oxytocin, English name is Oxytocin, structural formula:

[0003]

[0004] The molecular formula is: C 43 h 66 N 12 o 12 S 2 , the molecular weight is 1007.2.

[0005] Oxytocin is a polypeptide hormone uterotonic drug. Has the following functions:

[0006] (1) Stimulate the contraction of uterine smooth muscle, simulating the uterine contraction of normal childbirth, resulting in cervical dilatation, and the uterine response to oxytocin gradually increases during pregnancy and reaches its peak at full term. (2) Stimulate the smooth muscle contraction of the mammary gland, which helps the milk to be discharged from the breast, but does not increase the milk secretion of the mammary gland. It takes effect 3 to 5 minutes after intramuscular inject...

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