HPLC (High Performance Liquid Chromatography) method for simultaneously determining contents of seven components in blumea balsamifera

A kind of Ainaxiang content technology, applied in the direction of measuring device, material separation, analysis of materials, etc., can solve the problems of no Ainaxiang quality control research reports, etc., achieve the effect of simple method and high detection efficiency

Inactive Publication Date: 2018-01-09
GUIZHOU MEDICAL UNIV
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Problems solved by technology

[0003] Studies have found that, in addition to flavonoids, caffeoylquinic acid compounds are the main non-volatile components of Ainaxiang medicinal materials, and it has been reported in the literature that caffeoylquinic acid compounds have an

Method used

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  • HPLC (High Performance Liquid Chromatography) method for simultaneously determining contents of seven components in blumea balsamifera
  • HPLC (High Performance Liquid Chromatography) method for simultaneously determining contents of seven components in blumea balsamifera
  • HPLC (High Performance Liquid Chromatography) method for simultaneously determining contents of seven components in blumea balsamifera

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Embodiment 1

[0021] The invention provides a method for the simultaneous determination of 5-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, 1,3-O-dicafe Seven types of caffeoylquinic acid, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid and 4,5-O-dicaffeoylquinic acid The HPLC method of class composition, concrete implementation steps are as follows:

[0022] 1. Instruments and reagents

[0023] UltiMate 3000 high-performance liquid chromatography (Thermo Fisher Scientific), including system controller, infusion pump, degassing assembly, low-pressure gradient assembly, automatic sampler, column oven, temperature-controlled sample chamber, UV-VIS detector and Chromeleon Chromatography Data Workstation. EL204 electronic balance (Mettler-Toledo Instruments Shanghai Co., Ltd.). Beckman tabletop refrigerated centrifuge (USA). WP-UP-IV-20 ultrapure water machine (Sichuan Votel Technology Development Co., Ltd.).

[0024] Standard 5-O-caffeoylquinic acid (Sic...

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Abstract

The invention discloses an HPLC (High Performance Liquid Chromatography) method for simultaneously determining the contents of seven components in blumea balsamifera. Seven caffeoyl quinic acid components determined by the method are 5-O-caffeoyl quinic acid, 3-O-caffeoyl quinic acid, 4-O-caffeoyl quinic acid, 1,3-O-dicaffeoyl quinic acid, 3,4-O-dicaffeoyl quinic acid, 3,5-O-dicaffeoyl quinic acidand 4,5-O-dicaffeoyl quinic acid. The HPLC method comprises the following determining steps of (1) preparation of a standard solution; (2) preparation of a tested solution; (3) establishment of content determination standard curves of seven caffeoyl quinic acid components; (4) determination of the contents of seven caffeoyl quinic acid components in a sample. According to the HPLC method disclosed by the invention, the operation is simple and convenient, the precision degree, the repeatability and the stability are good, the accuracy is high, the contents of seven caffeoyl quinic acid components in the blumea balsamifera can be simultaneously determined, and higher practical value is obtained.

Description

technical field [0001] The invention relates to a content determination method of seven caffeoylquinic acid components in Ainaxiang, belonging to the technical field of traditional Chinese medicines. Background technique [0002] Blumea balsamifera (L.) DC is a plant of Asteraceae, which is widely distributed in Guizhou, Guangdong, Hainan and other places. It mainly contains volatile oil, flavonoids and other compounds. Its medicinal parts are its leaves and twigs, which have the functions of dispelling wind and dehumidification, warming the middle and relieving diarrhea, etc. Studies have shown that Ainaxiang has obvious anti-inflammatory effects and can be used to treat rheumatoid arthritis. [0003] Studies have found that, in addition to flavonoids, caffeoylquinic acid compounds are the main non-volatile components of Ainaxiang medicinal materials, and it has been reported in the literature that caffeoylquinic acid compounds have anti-inflammatory, antiviral and other b...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
Inventor 李勇军姚成芬蒋礼严平刘亭黄勇孙绪马雪刘春花陆苑孙佳
Owner GUIZHOU MEDICAL UNIV
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