Method for extracting shikimic acid and quinic acid from ginkgo leaves

A technology of shikimic acid and ginkgo biloba, which is applied in the separation/purification of carboxylic acid compounds, organic chemistry, bulk chemical production, etc., can solve the problems of large amount of wastewater treatment, environmental pollution, cumbersome operation, etc. The effect of reducing environmental pollution and convenient operation

Active Publication Date: 2020-06-05
SHAANXI JIAHE PHYTOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The object of the present invention is to provide a kind of method of developing shikimic acid and quinic acid in Ginkgo biloba, to overcome problems such as long time consumption, large amount of waste water treatment, environmental pollution, complicated operation that exist in the above-mentioned method, the whole process route of the pres...

Method used

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  • Method for extracting shikimic acid and quinic acid from ginkgo leaves
  • Method for extracting shikimic acid and quinic acid from ginkgo leaves
  • Method for extracting shikimic acid and quinic acid from ginkgo leaves

Examples

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Effect test

Embodiment 1

[0055] Cut 5 kg of fresh ginkgo leaves into pieces and add them to the extraction kettle. Use 5LpH=8 acetone solution as the entrainer. The pressure of the extraction kettle is 28MPa, the extraction temperature is 55°C, the extraction time is 1.5h, the pressure of No. 45°C, No. 2 separation tank pressure 6MPa, temperature 40°C. The extract (A) is obtained from No. 1 separation tank, the extract (A) is rich in chlorophyll, and the purification of product I chlorophyll can be continued. The extraction residue (B) is obtained in the extraction kettle. The extraction residue (B) is digested with alkaline water to inactivate the enzyme. For example, 5 times the amount of sodium bicarbonate aqueous solution can be used to cook at 100 ° C. After inactivating the enzyme, take it out, extract it twice with ethanol, combine the extracts, and concentrate the extracts to neutral. Filter the supernatant. Add polyamide to the supernatant [the mass ratio of supernatant to polyamide is 3:1]...

Embodiment 2

[0060] Cut 5 kg of fresh ginkgo leaves into pieces, add them to the extraction kettle, use 10LpH=8.5 acetone solution as the entrainer, the pressure of the extraction kettle is 30MPa, the temperature of the extraction kettle is 50°C, the extraction time is 2h, the pressure of No. 1 separation kettle is 8MPa, the temperature 40°C, No. 2 separation kettle pressure 6MPa, temperature 40°C. The extract (A) is obtained from No. 1 separation tank, the extract (A) is rich in chlorophyll, and the purification of product I chlorophyll can be continued. The extraction residue (B) is obtained in the extraction kettle. The extraction residue (B) is digested with alkaline water to inactivate the enzyme. After inactivating the enzyme, take out and extract twice with ethanol, combine the extracts, concentrate the extracts to be neutral, and filter to obtain the supernatant. Add polyamide to the supernatant [the mass ratio of supernatant to polyamide is 3:1], stir for 30 minutes and filter t...

Embodiment 3

[0063] Cut 5 kg of fresh ginkgo leaves into pieces, add them to the extraction kettle, use 8LpH=8 acetone solution as the entrainer, the pressure of the extraction kettle is 28MPa, the extraction temperature is 50°C, the extraction time is 1.5h, the pressure of No. 1 separation kettle is 8MPa, and the temperature 45°C, No. 2 separation tank pressure 6MPa, temperature 40°C. The extract (A) is obtained from No. 1 separation tank, the extract (A) is rich in chlorophyll, and the purification of product I chlorophyll can be continued. The extraction residue (B) is obtained in the extraction kettle. The extraction residue (B) is digested with alkaline water to inactivate the enzyme. After inactivating the enzyme, take out and extract twice with ethanol, combine the extracts, concentrate the extracts to be neutral, and filter to obtain the supernatant. Add polyamide to the supernatant [the mass ratio of supernatant to polyamide is 3:1] to obtain filtrate (C) (for standby) and preci...

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Abstract

The invention belongs to the field of organic acid extraction and separation, and relates to a method for extracting shikimic acid and quinic acid from ginkgo leaves. The problems of long consumed time, large wastewater treatment capacity, environmental pollution, complicated operation and the like in the existing method are solved; the chemical properties of each component in the ginkgo leaves are compared; a carbon dioxide supercritical extraction-extraction-membrane filtration-electrodialysis-crystallization method is combined for use; on the premise of commercial value, various active ingredients, including chlorophyll, ginkgetin, bilobalide, gingko polysaccharide and the like, in the ginkgo leaves are developed to the maximum extent, and finally, shikimic acid and quinic acid productsare obtained through separation, and the content of the shikimic acid and the content of the quinic acid are respectively greater than 98%.

Description

technical field [0001] The invention belongs to the field of extraction and separation of organic acids, and relates to a method for developing organic acids in ginkgo leaves, in particular to a method for extracting and separating shikimic acid and quinic acid from ginkgo leaves. Background technique [0002] Ginkgo biloba has been used as medicine for more than 600 years, and its efficacy was first recorded in "Shen Nong's Herbal Classic". Traditional medicine believes that ginkgo leaves and fruits are sweet, bitter, astringent, and flat in nature. Anti-alcoholic and other effects; ginkgo testa is sweet, warm in nature, beneficial to Qi and tonic. The chemical components of Ginkgo biloba mainly include flavonoids, terpenes, organic acids, phenolic acids, polysaccharides, etc. [0003] The organic acids in Ginkgo biloba mainly include the following types: D-glucuronic acid, shikimic acid, 6-hydroxykynurenic acid, quinic acid, etc. [0004] Ginkgo flavonoids and ginkgolid...

Claims

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Application Information

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IPC IPC(8): C07C51/42C07D311/40C07D487/22C07D493/22C08B37/00C07C62/32C07C62/04
CPCC07C51/42C07D487/22C07D311/40C07D493/22C08B37/0003C07C2601/14C07C2601/16C07C62/32C07C62/04Y02P20/54
Inventor 王晓莹程闪肖红张瑜
Owner SHAANXI JIAHE PHYTOCHEM
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