The present application belongs to the field of
leather industry production, and particularly relates to a method for
high absorption chrome tanning by using cross-linking
enzyme preparation. The method comprises the following steps:
water immersion, unhairing and liming, deliming,
softening,
enzyme-assisted tanning, and acid immersion tanning. The
enzyme-assisted tanning is assisted by using cross-linking enzyme preparation. The cross-linking enzyme preparation is not used in combination with the hydrolytic enzyme in the
softening step. The cross-linking enzyme comprises
glutamine transaminase (TG enzyme). The comprehensive activity of the cross-linking enzyme is controlled to be 1-30 U / ml in the application process, and the activity of the TG enzyme is controlled to be 1-20 U / ml. The process in the present application meets the
environmental protection requirements. Firstly, the tanning agent
absorption rate in the tanning process is successfully improved, and the waste
discharge amount in the tanning process is reduced. Secondly, the cleaner cross-linking enzyme preparation is successfully used to replace the chemical auxiliary agent to improve the tanning agent
absorption rate. Thirdly, the
process operation is simplified.