A glucuronolactone and a method for its preparation

Abstract

The application discloses a glucuronolactone and a preparation method thereof, and belongs to the technical field of fine chemical product preparation. The method takes potassium phytate extracted from corn soaking liquid or rice bran acid soaking liquid as raw material, obtains inositol and potassium dihydrogen phosphate after hydrolysis, separates the hydrolysis liquid through chromatography, obtains potassium dihydrogen phosphate by concentrating and crystallizing the salt phase, and the material phase is inositol solution. Enzymatic conversion reaction is carried out by using fermentation to obtain enzyme liquid, glucuronic acid liquid is obtained, glucuronolactone is obtained by concentrating and esterifying, and then the glucuronolactone is obtained by concentrating, refining and crystallizing. In the method, the yield of potassium phytate to inositol is greater than 95%, the molar conversion rate of inositol enzymatic conversion to glucuronic acid is greater than 95%, and the total yield is greater than 89%, which is much higher than the traditional yield, and the content of the obtained glucuronolactone is greater than 99.2%. The glucuronolactone is prepared by enzymatic conversion of the intermediate inositol, the by-product is high-value potassium dihydrogen phosphate, the whole has good economic benefits, and industrial production can be realized.

Description

Technical Field

[0001] This invention belongs to the field of fine chemical preparation technology, specifically relating to a glucuronide and its preparation method. Background Technology

[0002] Glucuronolactone, also known as D-glucuronolactone, D-glucuronic acid-γ-lactone, and D-glucuronic acid-6,3-lactone, is a compound known as D-glucuronolactone.

[0003] The chemical structural formula is as follows:

[0004]

[0005] Glucuronolactone's main functions are to enhance liver detoxification, restore or improve brain function, regulate immune function, nourish the skin, delay aging, improve hypoxia, eliminate fatigue, and enhance the control and coordination of various organ functions. It is primarily used in the pharmaceutical, daily chemical, food, and feed additive industries.

[0006] The traditional production method for glucuronide is the starch oxidation-hydrolysis method. Starch is used as raw material, and fuming nitric acid is added to oxidize the starch into oxidized starch. The oxidation solution is diluted, the pH is adjusted with acid, and then hydrolyzed under heat to obtain glucuronic acid. The hydrolysate is concentrated, and acetic acid or acetic anhydride is added for lactone formation. Crystallization is then carried out for approximately 75 hours to obtain crude glucuronide with a purity of 80-90%. After dissolving the crude product, activated carbon is added for decolorization, and recrystallization is performed to obtain the glucuronide product with a purity of over 99%, but the yield is only about 10%, meaning that only 1 ton of glucuronide is obtained from 10 tons of starch. The most prominent problem with this process is that the use of nitric acid oxidation not only oxidizes the primary hydroxyl groups but may also oxidize hydroxyl groups at other positions. Furthermore, the pressurized hydrolysis of oxidized starch, with prolonged time, also hydrolyzes the generated glucuronic acid, resulting in a decrease in the final glucuronide yield. This production method also consumes a large amount of nitric acid, produces numerous byproducts, generates large amounts of nitrogen dioxide, polluting the environment, and poses high safety risks during the production process. Summary of the Invention

[0007] The primary objective of this invention is to provide a method for preparing glucuronide. This method uses potassium phytate extracted from corn soaking solution or rice bran acid soaking solution as raw material. After hydrolysis, inositol and potassium dihydrogen phosphate are obtained. The hydrolysate is separated by chromatography, and the salt phase is concentrated and crystallized to obtain potassium dihydrogen phosphate. The material phase is an inositol solution. Then, an enzymatic conversion reaction is carried out using an enzyme solution obtained from the fermentation process to obtain a glucuronic acid solution. After concentration, acetic acid is added to esterify the glucuronide solution to obtain glucuronolactone. The glucuronolactone is then concentrated, purified, and crystallized to obtain the final product. In this method, the yield of potassium phytate to the intermediate inositol is greater than 95%, the molar conversion rate of inositol to glucuronolactone is greater than 95%, and the total yield is greater than 89%, which is significantly higher than the 10% yield of the traditional starch oxidation hydrolysis method. The obtained glucuronolactone content is greater than 99.2%, and the product indicators meet the requirements of the Chinese Pharmacopoeia standards.

[0008] The second objective of this invention is to provide a method for preparing glucuronide. This method uses enzymes to convert the intermediate product inositol to obtain glucuronide, which has the advantages of turning waste into treasure, circular economy, environmental friendliness, high yield, and safe production. While producing glucuronide, potassium dihydrogen phosphate, which has high economic value, is produced as a byproduct. Overall, it has good economic benefits and can be industrialized.

[0009] This invention is achieved through the following technical solution:

[0010] A method for preparing glucuronide includes the following steps:

[0011] S1. Potassium dihydrogen phosphate solution and inositol solution are prepared by selecting potassium phytate solution extracted from corn soaking solution or rice bran acid soaking solution as raw materials. The inositol solution is concentrated under reduced pressure until the inositol mass fraction reaches 9.5 wt%-12.2 wt%, and then placed in a conversion device for enzymatic conversion reaction to obtain a conversion solution containing glucuronic acid.

[0012] S2. Filter the conversion solution containing glucuronic acid through a ceramic membrane to obtain a clear liquid and a heavy liquid;

[0013] After washing with deionized water, the glucuronic acid mass fraction in the heavy liquid is no higher than 0.5 wt%.

[0014] Acetic acid is added to the clear liquid, and the liquid is concentrated under vacuum using a rotary evaporator to 20%-25% of its original volume. Concentration is then stopped. The concentrated liquid is then cooled to 0-15℃ using cold water at 8℃-10℃ for crystallization, filtration, and drying to obtain the glucuronide product.

[0015] Preferably, in S1, the enzymatic conversion reaction process is as follows:

[0016] A 30L fermenter was used. Culture medium was added and sterilized. Recombinant engineered bacteria were then inoculated and fermented to obtain 18-20L of fermentation broth. After fermentation, the OD (distillate) was discharged from the fermenter. 600 Values ​​between 100 and 120;

[0017] b. The fermentation broth is filtered through a ceramic membrane to obtain a clear liquid and a heavy liquid. During filtration, the material temperature is controlled below 35℃ and the feed pressure is 0.3-0.5 MPa.

[0018] When the volume of the heavy liquid drops to 8-10L, add 10-20L of physiological saline to wash the bacterial cells. After washing, stop the machine and collect the heavy liquid, which should be 8-10L in volume. The heavy liquid OD... 600 Values ​​are between 200 and 240;

[0019] The heavy liquid is frozen at -5℃ or subjected to cell disruption treatment with the addition of a cell disrupting agent to obtain an enzyme solution containing inositol oxidase; the enzyme solution is stored at 8-10℃; the cell disruption agent is hexadecyltrimethylammonium bromide.

[0020] Add the enzyme solution to the inositol solution at a ratio of 70-100 OD / mL, control the temperature at 35-37℃, stir at 100-200 rpm, and introduce air to carry out enzymatic conversion. The molar conversion rate of inositol to glucuronic acid is >95%, and the concentration of glucuronic acid during conversion is 9.0%-11.5%.

[0021] Equation for enzyme-catalyzed conversion reaction:

[0022] .

[0023] Preferably, in step S1, the feed pressure is controlled at 0.3-0.5 MPa during the ceramic membrane filtration process;

[0024] The volume ratio of deionized water added to the heavy liquid is 1:2-4;

[0025] The volume ratio of acetic acid added to the clarified liquid is 4wt%-7wt%;

[0026] The vacuum concentration temperature is 50-80℃, and the vacuum degree is greater than -0.09Mpa;

[0027] When the concentrate is cooled by ice water, the cooling gradient is 5-10℃ / h;

[0028] The reaction equation for adding acetic acid to the clear liquid is as follows:

[0029] .

[0030] Preferably, the process for preparing potassium dihydrogen phosphate solution and inositol solution using potassium phytate solution extracted from corn soaking solution or rice bran acid soaking solution as raw material is as follows:

[0031] A. Raw material hydrolysis:

[0032] Potassium phytate solution extracted from corn soaking solution or rice bran acid soaking solution was selected as raw material and subjected to heated hydrolysis treatment to obtain mixed mother liquor;

[0033] The mixed mother liquor comprises a mixed mother liquor with a mass fraction of 2.4 wt%-3.07 wt% inositol and a mass fraction of 11.16 wt%-13.95 wt% potassium dihydrogen phosphate;

[0034] B. Decolorization and concentration under reduced pressure:

[0035] After decolorizing the mixed mother liquor by stirring with activated carbon, it was first concentrated, then cooled and crystallized, and then centrifuged to obtain potassium dihydrogen phosphate crystals and centrifuged mother liquor.

[0036] The centrifuged mother liquor comprises a mixed mother liquor with a mass fraction of 3.5 wt%-3.8 wt% inositol and a mass fraction of 11.0 wt%-14 wt% potassium dihydrogen phosphate;

[0037] C. Separation by chromatographic column:

[0038] After separation by chromatographic column chromatography, potassium dihydrogen phosphate solution and inositol solution were obtained respectively from the centrifuged mother liquor.

[0039] The main purpose of the above-mentioned raw material hydrolysis step is to hydrolyze the potassium phytate solution at a certain temperature, thereby breaking down the potassium phytate molecular chains to obtain inositol and potassium dihydrogen phosphate. The reaction equation for this step is as follows:

[0040] .

[0041] Preferably, in the hydrolysis of the raw materials, the potassium phytate solution contains a potassium phytate mass fraction of 12wt%-16wt% and a pH value of 4.2-4.5.

[0042] The heating and hydrolysis process is as follows: after heating to 155-160℃, maintain the temperature for 8-10 hours for hydrolysis.

[0043] Preferably, in the decolorization and vacuum concentration process, the mass fraction ratio of the mixed mother liquor to activated carbon is 1:0.3wt%-0.5wt%.

[0044] The activated carbon stirring and decolorization time is 40-60 minutes.

[0045] When the concentration and crystallization reaches a specific gravity of 1.38-1.4, a portion of potassium dihydrogen phosphate crystals are obtained; then the temperature is lowered to no higher than 30°C and centrifuged to obtain another portion of potassium dihydrogen phosphate crystals, which are then mixed with the previous crystals.

[0046] The mass fraction of inositol in the potassium dihydrogen phosphate crystals is no greater than 0.2 wt%.

[0047] Preferably, in the column chromatography separation, the potassium dihydrogen phosphate feed solution is concentrated and crystallized to obtain potassium dihydrogen phosphate crystals, which are then mixed with potassium dihydrogen phosphate crystals in B and dried to obtain the finished potassium dihydrogen phosphate product.

[0048] The separation is performed using a potassium-type resin chromatographic column;

[0049] The mass concentration of potassium dihydrogen phosphate in the potassium dihydrogen phosphate solution is 16 wt%-19 wt%.

[0050] Preferably, in the chromatographic column separation, the inositol mass concentration in the inositol feed solution is not greater than 0.2 wt%, and the purity is not less than 93%.

[0051] Preferably, the inositol concentration in the inositol solution is 2.8 wt%-3.2 wt%, and the purity is 95%-97%.

[0052] A glucuronide is obtained using the preparation method described above.

[0053] Compared with the prior art, the present invention has at least the following technical effects:

[0054] (I) This invention provides a method for preparing glucuronide. The method uses potassium phytate extracted from corn soaking solution or rice bran acid soaking solution as raw material. After hydrolysis, inositol and potassium dihydrogen phosphate are obtained. The hydrolysate is separated by chromatography, and the salt phase is concentrated and crystallized to obtain potassium dihydrogen phosphate. The material phase is an inositol solution. Then, an enzymatic conversion reaction is carried out using an enzyme solution obtained from the fermentation process to obtain a glucuronic acid solution. Acetic acid is added during the concentration of the glucuronic acid solution for esterification to obtain glucuronolactone. The solution is then concentrated, purified, and crystallized to obtain the final product. In this method, the yield of potassium phytate to intermediate inositol is greater than 95%, the molar conversion rate of inositol to glucuronolactone is greater than 95%, and the total yield is greater than 89%, which is much higher than the 10% yield of the traditional starch oxidation hydrolysis method. The content of the obtained glucuronolactone is greater than 99.2%.

[0055] (ii) This method produces glucuronolactone by enzymatically converting the intermediate product inositol. It has the advantages of circular economy, turning waste into treasure, environmental friendliness, high yield and safe production. While producing glucuronolactone, it also produces potassium dihydrogen phosphate, which has high economic value as a by-product. Overall, it has good economic benefits and can be industrialized. Attached Figure Description

[0056] Figure 1 The liquid chromatography chromatogram of the inositol enzyme-catalyzed reaction solution;

[0057] Figure 2 Infrared spectra of glucuronolactone finished product and standard product;

[0058] Figure 3 Infrared spectrum of glucuronolactone standard. Detailed Implementation

[0059] The embodiments of the present invention will be described in detail below with reference to the examples. However, those skilled in the art will understand that the following examples are only for illustrating the present invention and should not be regarded as limiting the scope of the present invention. Specific conditions not specified in the examples shall be carried out according to conventional conditions or conditions recommended by the manufacturer. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0060] One specific embodiment of the present invention is as follows:

[0061] Example 1: Treatment using potassium phytate as an example

[0062] A method for producing glucuronolactone using potassium phytate as a raw material includes the following steps:

[0063] 1. Potassium phytate is extracted from corn soaking solution or rice bran acid soaking solution, and is classified as hexapotassium phytate with a pH of 4.2-4.5 and a concentration of 12wt%-16wt%.

[0064] 2. A potassium phytate solution with a mass concentration of 12 wt%-16 wt% is fed into a reaction vessel, heated to 155-160℃, and hydrolyzed for 8-10 hours to obtain a mixed solution of inositol and potassium dihydrogen phosphate. The inositol mass concentration is 2.4 wt%-3.07 wt%, the potassium dihydrogen phosphate content is 11.16 wt%-13.95 wt%, and the hydrolysis rate is greater than 99%.

[0065] Reaction equation:

[0066] .

[0067] 3. Add activated carbon at a concentration of 1:0.3 wt%-0.5 wt% to the hydrolysate and stir for decolorization. Control the decolorization time to 40-60 minutes. After decolorization, use a self-cleaning filter to remove activated carbon and suspended impurities.

[0068] 4. The decolorizing solution is concentrated under reduced pressure until the specific gravity reaches 1.38-1.4, then discharged into a crystallization tank. The solution is cooled for crystallization. Since the concentration of potassium dihydrogen phosphate in the concentrated solution is much higher than that of inositol, the crystals obtained in this step are mainly potassium dihydrogen phosphate. The solution temperature is lowered to below 30℃, and potassium dihydrogen phosphate crystals are obtained by centrifugation. The inositol concentration in the potassium dihydrogen phosphate is less than 0.2 wt%. The centrifugation mother liquor is a mixed solution of inositol and potassium dihydrogen phosphate, with an inositol concentration of 3.5 wt%-3.8 wt% and a potassium dihydrogen phosphate concentration of 11.0 wt%-14 wt%.

[0069] 5. The centrifuged mother liquor was separated using a potassium-type resin chromatographic column to obtain an inositol feed phase and a potassium dihydrogen phosphate feed phase. The inositol feed phase had an inositol concentration of 2.8 wt%-3.2 wt% and a purity of 95%-97%. The potassium dihydrogen phosphate feed phase had a potassium dihydrogen phosphate concentration of 16 wt%-19 wt%, an inositol concentration of less than 0.2%, and a purity greater than 93%.

[0070] 6. The potassium dihydrogen phosphate solution is concentrated and crystallized to obtain potassium dihydrogen phosphate crystals, which are then combined with the potassium dihydrogen phosphate crystals obtained in step 4 and dried to obtain the finished potassium dihydrogen phosphate product.

[0071] 7. The inositol solution is concentrated under reduced pressure until the inositol mass concentration reaches 9.5 wt%-12.2 wt%. The solution is then discharged into a conversion tank to await the addition of inositol oxidase for enzymatic conversion.

[0072] 8. Using a 30L fermenter with a carbon source as the culture medium, inoculate with recombinant engineered bacteria for fermentation culture to obtain 18-20L of fermentation broth. Remove from the fermenter. 600 The value is between 100 and 120.

[0073] 9. Filter the fermentation broth through a ceramic membrane. During filtration, control the material temperature below 35℃ and the feed pressure at 0.3-0.5 MPa. When the heavy liquid volume drops to 8-10 L, add 10-20 L of physiological saline to wash the cells. After washing, stop the machine and collect the heavy liquid, which should be 8-10 L in volume. Heavy liquid OD 600 The value is between 200-240. The heavy liquid is subjected to freeze-thaw treatment or CTAB reagent is added to disrupt the cell walls, releasing the enzymes within the bacteria to obtain an enzyme solution containing inositol oxidase. The enzyme solution is stored at 8-10℃.

[0074] 10. Add the enzyme solution to the inositol solution at a ratio of 70-100 OD / mL, control the temperature at 35-37℃, stir at 100-200 rpm, and introduce air to carry out enzymatic conversion. The molar conversion rate of inositol to glucuronic acid is >95%, and the mass concentration of glucuronic acid in the conversion is 9.0wt%-11.5wt%.

[0075] The reaction equation is:

[0076] .

[0077] 11. Filter the conversion solution containing glucuronic acid through a ceramic membrane to remove insoluble impurities. During membrane filtration, control the feed pressure at 0.3-0.5 MPa. After filtering to the minimum volume of heavy liquid, wash the heavy liquid with 2-4 times its volume of deionized water, controlling the glucuronic acid content in the heavy liquid to below 0.5 wt%.

[0078] 12. Add 4 wt%-7 wt% acetic acid to the membrane filtration clarified liquid, and then concentrate it under vacuum using a rotary evaporator, controlling the concentration temperature at 50-80℃ and the vacuum degree > -0.09MPa. During the concentration process, glucuronide is converted to glucuronolactone.

[0079] The reaction equation is:

[0080] .

[0081] 13. When the concentration reaches 20-25% of the original volume, stop the concentration process. Cool the concentrated solution to 0-15℃ using ice water for crystallization, controlling the cooling rate at 5-10℃ / h. Filter and dry the crystallized solution to obtain the glucuronolactone product.

[0082] like Figure 1 The figure shows the liquid chromatography chromatogram of the inositol enzymatic reaction solution. The chromatogram indicates that the inositol mass concentration in the conversion system was 10.8 wt%, the residual inositol after conversion was 0.349 wt%, and the conversion rate was 96.77%.

[0083] like Figure 2 The image shows the infrared spectrum of the finished product and the infrared spectrum of the standard product of glucuronolactone.

[0084] like Figure 3 The image shown is the infrared spectrum of glucuronolactone standard.

[0085] Experimental example:

[0086] Experimental group: prepared using the method described in Example 1 above.

[0087] Result 1: Standard test results for the experimental group:

[0088] project Standard requirements Sample test results Properties White crystalline powder White crystalline powder Melting point 170.0-176.0℃ 172.5-173.5℃ Specific curl +18.0-20.0 +18.6 Loss on drying ≤0.5% ＜0.10% heavy metal ≤10PPM <10PPM content 98.5%-102.0% 100.1%

[0089] Result 2: Statistical table of yield calculation for each step in the experimental group:

[0090] 1. Yield statistics for the heating hydrolysis step:

[0091]

[0092] 2. Desalting yield of inositol hydrolysate:

[0093]

[0094] 3. Yield of inositol solution enzymatic conversion of glucuronolactone:

[0095]

[0096] 4. Total yield of potassium phytate to glucuronolactone:

[0097]

[0098] Finally, it should be noted that the above description is merely a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for preparing glucuronide, characterized in that, Includes the following steps: S1. Potassium dihydrogen phosphate solution and inositol solution are prepared by selecting potassium phytate solution extracted from corn soaking solution or rice bran acid soaking solution as raw materials. The inositol solution is concentrated under reduced pressure until the inositol mass fraction reaches 9.5 wt%-12.2 wt%, and then placed in a conversion device for enzymatic conversion reaction to obtain a conversion solution containing glucuronic acid. S2. Filter the conversion solution containing glucuronic acid through a ceramic membrane to obtain a clear liquid and a heavy liquid; After washing with deionized water, the glucuronic acid mass fraction in the heavy liquid is no higher than 0.5 wt%. Acetic acid was added to the clear liquid, and the liquid was concentrated under vacuum using a rotary evaporator to 20%-25% of the original volume. Concentration was then stopped. The concentrated liquid was then cooled to 0-15°C with ice water to crystallize the liquid, with a cooling gradient of 5-10°C / h. The liquid was then filtered and dried to obtain the glucuronide product. The enzymatic conversion reaction process is as follows: Using a 30L fermenter, add culture medium and sterilize it. Inoculate with recombinant engineered bacteria for fermentation culture, obtaining 18-20L of fermentation broth. After fermentation, discharge the OD from the fermenter. 600 Values ​​between 100 and 120; b. The fermentation broth is filtered through a ceramic membrane to obtain a clear liquid and a heavy liquid. During filtration, the material temperature is controlled below 35℃ and the feed pressure is 0.3-0.5MPa. When the volume of the heavy liquid drops to 8-10L, add 10-20L of physiological saline to wash the bacterial cells. After washing, stop the machine and collect the heavy liquid, which should be 8-10L in volume. The heavy liquid OD... 600 Values ​​are between 200 and 240; The heavy liquid is frozen at -5℃ or subjected to cell disruption treatment with the addition of a cell disrupting agent to obtain an enzyme solution containing inositol oxidase; the enzyme solution is stored at 8-10℃; the cell disruption agent is hexadecyltrimethylammonium bromide. Add the enzyme solution to the inositol solution at a ratio of 70-100 OD / mL, control the temperature at 35-37℃, stir at 100-200 rpm, and introduce air to carry out enzymatic conversion. The molar conversion rate of inositol to glucuronic acid is >95%, and the concentration of glucuronic acid after conversion is 9.0%-11.5%. Equation for enzyme-catalyzed conversion reaction: During the ceramic membrane filtration process, the feed pressure is controlled at 0.3-0.5 MPa; The volume ratio of deionized water added to the heavy liquid is 1:2-4; The volume ratio of acetic acid added to the clarified liquid is 4%-7%; The vacuum concentration temperature is 50-80℃, and the vacuum degree is greater than -0.09MPa; The reaction equation for adding acetic acid to the clear liquid is as follows: ； The process for preparing potassium dihydrogen phosphate solution and inositol solution using potassium phytate solution extracted from corn soaking solution or rice bran acid soaking solution as raw material is as follows: A. Raw material hydrolysis: Potassium phytate solution extracted from corn soaking solution or rice bran acid soaking solution was selected as raw material and subjected to heated hydrolysis treatment to obtain mixed mother liquor; The mixed mother liquor comprises a mixed mother liquor with a mass fraction of 2.4 wt%-3.07 wt% inositol and a mass fraction of 11.16 wt%-13.95 wt% potassium dihydrogen phosphate; B. Decolorization and concentration under reduced pressure: After decolorizing the mixed mother liquor by stirring with activated carbon, it was first concentrated, then cooled and crystallized, and then centrifuged to obtain potassium dihydrogen phosphate crystals and centrifuged mother liquor. The centrifuged mother liquor comprises a mixed mother liquor with a mass fraction of 3.5 wt%-3.8 wt% inositol and a mass fraction of 11.0 wt%-14 wt% potassium dihydrogen phosphate; C. Separation by chromatographic column: After separation by chromatographic column chromatography, potassium dihydrogen phosphate solution and inositol solution were obtained respectively. In the hydrolysis of the raw materials, the potassium phytate solution contains a potassium phytate mass fraction of 12wt%-16wt% and a pH value of 4.2-4.

5. The heating hydrolysis process is as follows: after heating to 155-160℃, hydrolyze at this temperature for 8-10 hours. In the decolorization and vacuum concentration process, the mass fraction ratio of the mixed mother liquor to activated carbon is 1:0.3wt%-0.5wt%. The activated carbon stirring and decolorization time is 40-60 minutes. When the concentration is brought to a specific gravity of 1.38-1.4, a portion of potassium dihydrogen phosphate crystals are obtained; then the temperature is lowered to no higher than 30°C and centrifuged to obtain another portion of potassium dihydrogen phosphate crystals, which are then mixed with the previous crystals. The mass fraction of inositol in the potassium dihydrogen phosphate crystals is no greater than 0.2 wt%. In the chromatographic column separation, the potassium dihydrogen phosphate feed solution is concentrated and crystallized to obtain potassium dihydrogen phosphate crystals, which are then mixed with potassium dihydrogen phosphate crystals in B and dried to obtain the finished potassium dihydrogen phosphate product. The separation is performed using a potassium-type resin chromatographic column; The mass concentration of potassium dihydrogen phosphate in the potassium dihydrogen phosphate solution is 16 wt%-19 wt%. The inositol solution contains an inositol concentration of 2.8 wt%-3.2 wt% and a purity of 95%-97%.

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