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Method for extracting and refining rhizobia exocellular polysaccharide

An extracellular polysaccharide and purification method technology, applied in the biological field, can solve the problems of many steps and high extraction cost, and achieve the effects of less process steps, high purity and low cost

Active Publication Date: 2011-01-12
天津强微特生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the disadvantages of many steps, high extraction cost, and the finished product contains neutral and alkaline polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. The method for extracting and refining rhizobia gum from the fermentation broth of rhizobia is completed by the following process steps:

[0031] Preparation of fermentation broth: The fermentation strain is Rhizobium astragula (CICC: 20026), and the slant medium formula: beef extract 0.5%, peptone 3.9%, glucose 0.1%, sodium chloride 0.5%, agar 1.5~2.0 %, pH7.2, culture temperature 30℃; The preparation method of shaking seed culture medium is the same as above except that agar is not added; Fermentation medium formula: NaCl 0.01%, glucose 2%, K 2 HPO 4 0.4%, MgSO 4 .7H 2 O 0.5%, soybean meal 2%, Na 2 Mo 4 .2H 2 O0.002%, pH7.0, culture temperature 30°C; after 18 hours of seed cultivation, 5% of the inoculum was inserted into the fermentor, after 40 hours of cultivation, the enzyme was inactivated at 60°C for 30 minutes, and then placed in the tank to obtain the fermentation broth.

[0032] 1. Dilution pretreatment:

[0033] Add 3 times the volume of distilled water t...

Embodiment 2

[0045] The method of extracting and refining rhizobia gum from rhizobia fermentation broth is completed by the following process steps:

[0046] Preparation of fermentation broth: the fermentation strain is Rhizobiumradiobacter (CICC: 10270), and the slant medium formula: peptone 5g beef extract 3g, sodium chloride 5g agar 15g, distilled water 1000ml to adjust the pH to 7.0; The preparation method of the shaker seed culture medium is the same as above except that agar is not added; the fermentation medium formula: NaCl 0.01%, glucose 2%, K 2 HPO 4 0.4%, MgSO 4 .7H 2 O 0.5%, soybean meal 2%, Na 2 Mo 4 .2H 2 O0.002%, pH 7.0, culture temperature 30°C; after 20 hours of seed cultivation, 3% of the inoculum was inserted into the fermentor, after 48 hours of cultivation, the enzyme was inactivated at 60°C for 30 minutes, and then placed in the tank to obtain the fermentation broth.

[0047] 1. Dilution pretreatment

[0048] Add 2 times the volume of distilled water to the fermentation brot...

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PUM

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Abstract

The invention discloses a method for extracting and refining rhizobia exocellular polysaccharide. The method is finished by the following steps: dilution pretreatment, flocculation and protein removal, ion exchange, concentration and salt removal, alcohol precipitation with alcohol, and vacuum drying. The method for extracting and refining the rhizobia exocellular polysaccharide from rhizobia fermentation liquor has the characteristics of few process steps, high efficiency, low cost and suitability for industrial production; and the prepared exocellular polysaccharide has high purity.

Description

Technical field [0001] The invention relates to a method for extracting and purifying rhizobia extracellular polysaccharides from rhizobia fermentation broth, and belongs to the field of biotechnology. Background technique [0002] Microbial polysaccharides are biopolymers produced by microorganisms such as bacteria, fungi and cyanobacteria in the metabolic process that have a protective effect on microorganisms. According to different forms of existence in cells, they are usually divided into three categories: ① Cells that constitute microbial cell components Endopolysaccharides; ②wall polysaccharides that adhere to the cell surface; ③extracellular polysaccharides secreted into the culture medium, including microcapsules, capsules, mucous layers and bacterial micelles. Microbial polysaccharides have a wide range of applications, such as microbial flocculants in many areas of industrial production and life, biochemical profile control agents in microbial oil extraction, food addi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12P19/04C12R1/41
Inventor 郑春阳魏国祥张巍
Owner 天津强微特生物科技有限公司
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